Liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tablets
Simple, accurate and precise reversed-phase liquid chromatographic (LC) and spectrophotometric methods have been developed and validated for the determination of erythromycin stearate (ERS) and trimethoprim (TMP) in mixture. In LC method, chromatographic separation was achieved on a Symmetry® Waters...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Cairo University
2011-12-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110093111000111 |
| _version_ | 1828079812818763776 |
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| author | Sonia T. Hassib Awatef E. Farag Ehab F. Elkady |
| author_facet | Sonia T. Hassib Awatef E. Farag Ehab F. Elkady |
| author_sort | Sonia T. Hassib |
| collection | DOAJ |
| description | Simple, accurate and precise reversed-phase liquid chromatographic (LC) and spectrophotometric methods have been developed and validated for the determination of erythromycin stearate (ERS) and trimethoprim (TMP) in mixture. In LC method, chromatographic separation was achieved on a Symmetry® Waters C18 column (150 × 4.6 mm, 5 μm) based on isocratic elution using a mobile phase consisting of potassium dihydrogen phosphate buffer pH (9):acetonitrile:water (25:100:50, v/v/v) at a flow rate of 1.6 ml min−1 with UV detection at 210 nm for ERS and 280 nm for TMP. Besides, two spectrophotometric methods were applied after reaction with perchloric acid (12 M) which gives a colored product with ERS. Then, the spectral interference between the colored product of ERS and TMP was resolved by either ratio spectra derivative spectrophotometry in the first spectrophotometric method or chemometric techniques, namely classical least-squares (CLS), principal component regression (PCR) and partial least-squares regression (PLS) in the second spectrophotometric method. The results were statistically compared using one-way analysis of variance (ANOVA). The methods developed were satisfactorily applied to the analysis of the pharmaceutical preparation containing the two drugs and proved to be specific and accurate for the quality control of the cited drugs in pharmaceutical dosage forms. |
| first_indexed | 2024-04-11T03:08:09Z |
| format | Article |
| id | doaj.art-3bbf1e8d4a7a4bd1bea20b8075c80d3f |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T03:08:09Z |
| publishDate | 2011-12-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-3bbf1e8d4a7a4bd1bea20b8075c80d3f2023-01-02T12:24:22ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312011-12-01492818910.1016/j.bfopcu.2011.07.008Liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tabletsSonia T. HassibAwatef E. FaragEhab F. ElkadySimple, accurate and precise reversed-phase liquid chromatographic (LC) and spectrophotometric methods have been developed and validated for the determination of erythromycin stearate (ERS) and trimethoprim (TMP) in mixture. In LC method, chromatographic separation was achieved on a Symmetry® Waters C18 column (150 × 4.6 mm, 5 μm) based on isocratic elution using a mobile phase consisting of potassium dihydrogen phosphate buffer pH (9):acetonitrile:water (25:100:50, v/v/v) at a flow rate of 1.6 ml min−1 with UV detection at 210 nm for ERS and 280 nm for TMP. Besides, two spectrophotometric methods were applied after reaction with perchloric acid (12 M) which gives a colored product with ERS. Then, the spectral interference between the colored product of ERS and TMP was resolved by either ratio spectra derivative spectrophotometry in the first spectrophotometric method or chemometric techniques, namely classical least-squares (CLS), principal component regression (PCR) and partial least-squares regression (PLS) in the second spectrophotometric method. The results were statistically compared using one-way analysis of variance (ANOVA). The methods developed were satisfactorily applied to the analysis of the pharmaceutical preparation containing the two drugs and proved to be specific and accurate for the quality control of the cited drugs in pharmaceutical dosage forms.http://www.sciencedirect.com/science/article/pii/S1110093111000111ChemometryErythromycin stearateReversed-phase liquid chromatographySpectrophotometryTrimethoprim |
| spellingShingle | Sonia T. Hassib Awatef E. Farag Ehab F. Elkady Liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tablets Bulletin of Faculty of Pharmacy Cairo University Chemometry Erythromycin stearate Reversed-phase liquid chromatography Spectrophotometry Trimethoprim |
| title | Liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tablets |
| title_full | Liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tablets |
| title_fullStr | Liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tablets |
| title_full_unstemmed | Liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tablets |
| title_short | Liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tablets |
| title_sort | liquid chromatographic and spectrophotometric methods for the determination of erythromycin stearate and trimethoprim in tablets |
| topic | Chemometry Erythromycin stearate Reversed-phase liquid chromatography Spectrophotometry Trimethoprim |
| url | http://www.sciencedirect.com/science/article/pii/S1110093111000111 |
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