The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens
Abstract Sphingolipid long chain bases (LCBs) are building blocks of sphingolipids and can serve as signalling molecules, but also have antimicrobial activity and were effective in reducing growth of a range of human pathogens. In plants, LCBs are linked to cell death processes and the regulation of...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-01-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-05083-4 |
| _version_ | 1819260969825599488 |
|---|---|
| author | René Glenz Agnes Kaiping Delia Göpfert Hannah Weber Benjamin Lambour Marvin Sylvester Christian Fröschel Martin J. Mueller Mohamed Osman Frank Waller |
| author_facet | René Glenz Agnes Kaiping Delia Göpfert Hannah Weber Benjamin Lambour Marvin Sylvester Christian Fröschel Martin J. Mueller Mohamed Osman Frank Waller |
| author_sort | René Glenz |
| collection | DOAJ |
| description | Abstract Sphingolipid long chain bases (LCBs) are building blocks of sphingolipids and can serve as signalling molecules, but also have antimicrobial activity and were effective in reducing growth of a range of human pathogens. In plants, LCBs are linked to cell death processes and the regulation of defence reactions against pathogens, but their role in directly influencing growth of plant-interacting microorganisms has received little attention. Therefore, we tested the major plant LCB phytosphingosine in in vitro tests with the plant pathogenic fungi Verticillium longisporum, Fusarium graminearum and Sclerotinia sclerotiorum, the plant symbiotic fungal endophyte Serendipita indica, the bacterial pathogens Pseudomonas syringae pv. tomato (Pst), Agrobacterium tumefaciens, and the related beneficial strain Rhizobium radiobacter. Phytosphingosine inhibited growth of these organisms at micromolar concentrations. Among the fungal pathogens, S. sclerotiorum was the most, and F. graminearum was the least sensitive. 15.9 μg/mL phytosphingosine effectively killed 95% of the three bacterial species. Plant disease symptoms and growth of Pst were also inhibited by phytosphingosine when co-infiltrated into Arabidopsis leaves, with no visible negative effect on host tissue. Taken together, we demonstrate that the plant LCB phytosphingosine inhibits growth of plant-interacting microorganisms. We discuss the potential of elevated LCB levels to enhance plant pathogen resistance. |
| first_indexed | 2024-12-23T19:34:21Z |
| format | Article |
| id | doaj.art-3bc715ffb785416abb12a280844e79aa |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T19:34:21Z |
| publishDate | 2022-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3bc715ffb785416abb12a280844e79aa2022-12-21T17:33:50ZengNature PortfolioScientific Reports2045-23222022-01-011211910.1038/s41598-022-05083-4The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogensRené Glenz0Agnes Kaiping1Delia Göpfert2Hannah Weber3Benjamin Lambour4Marvin Sylvester5Christian Fröschel6Martin J. Mueller7Mohamed Osman8Frank Waller9Julius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgJulius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgJulius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgJulius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgJulius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgJulius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgJulius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgJulius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgJulius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgJulius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität WürzburgAbstract Sphingolipid long chain bases (LCBs) are building blocks of sphingolipids and can serve as signalling molecules, but also have antimicrobial activity and were effective in reducing growth of a range of human pathogens. In plants, LCBs are linked to cell death processes and the regulation of defence reactions against pathogens, but their role in directly influencing growth of plant-interacting microorganisms has received little attention. Therefore, we tested the major plant LCB phytosphingosine in in vitro tests with the plant pathogenic fungi Verticillium longisporum, Fusarium graminearum and Sclerotinia sclerotiorum, the plant symbiotic fungal endophyte Serendipita indica, the bacterial pathogens Pseudomonas syringae pv. tomato (Pst), Agrobacterium tumefaciens, and the related beneficial strain Rhizobium radiobacter. Phytosphingosine inhibited growth of these organisms at micromolar concentrations. Among the fungal pathogens, S. sclerotiorum was the most, and F. graminearum was the least sensitive. 15.9 μg/mL phytosphingosine effectively killed 95% of the three bacterial species. Plant disease symptoms and growth of Pst were also inhibited by phytosphingosine when co-infiltrated into Arabidopsis leaves, with no visible negative effect on host tissue. Taken together, we demonstrate that the plant LCB phytosphingosine inhibits growth of plant-interacting microorganisms. We discuss the potential of elevated LCB levels to enhance plant pathogen resistance.https://doi.org/10.1038/s41598-022-05083-4 |
| spellingShingle | René Glenz Agnes Kaiping Delia Göpfert Hannah Weber Benjamin Lambour Marvin Sylvester Christian Fröschel Martin J. Mueller Mohamed Osman Frank Waller The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens Scientific Reports |
| title | The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens |
| title_full | The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens |
| title_fullStr | The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens |
| title_full_unstemmed | The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens |
| title_short | The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens |
| title_sort | major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens |
| url | https://doi.org/10.1038/s41598-022-05083-4 |
| work_keys_str_mv | AT reneglenz themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT agneskaiping themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT deliagopfert themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT hannahweber themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT benjaminlambour themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT marvinsylvester themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT christianfroschel themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT martinjmueller themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT mohamedosman themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT frankwaller themajorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT reneglenz majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT agneskaiping majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT deliagopfert majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT hannahweber majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT benjaminlambour majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT marvinsylvester majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT christianfroschel majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT martinjmueller majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT mohamedosman majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens AT frankwaller majorplantsphingolipidlongchainbasephytosphingosineinhibitsgrowthofbacterialandfungalplantpathogens |