Protective effect of nimesulide on the external ear damage induced by staphylococcus aureus inoculation in rats
Since the external ear is covered with skin, infections that cause otitis externa are often produced by distinct skin flora in several different areas. Staphylococcus aureus (S. aureus) reproduces the most out of all bacteria isolated from external auditory canal skin culture samples. Proinflammator...
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| Language: | English |
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Society of Turaz Bilim
2021-06-01
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| Series: | Medicine Science |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=57001 |
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| author | Ertugrul Erhan Ismail Salcan Muhammet Dilber Sumeyye Akyuz Bulent Dabanlioglu Bahadir Suleyman Taha Abdulkadir Coban Halis Suleyman Renad Mammadov |
| author_facet | Ertugrul Erhan Ismail Salcan Muhammet Dilber Sumeyye Akyuz Bulent Dabanlioglu Bahadir Suleyman Taha Abdulkadir Coban Halis Suleyman Renad Mammadov |
| author_sort | Ertugrul Erhan |
| collection | DOAJ |
| description | Since the external ear is covered with skin, infections that cause otitis externa are often produced by distinct skin flora in several different areas. Staphylococcus aureus (S. aureus) reproduces the most out of all bacteria isolated from external auditory canal skin culture samples. Proinflammatory cytokines are the main components responsible for tissue damage pathogenesis due to S. aureus. Nimesulide is a nonsteroidal anti-inflammatory drug that selectively inhibits cyclooxygenase-2 and also demonstrates antioxidant properties. The present study aimed to examine the antibacterial activity of nimesulide against S. aureus and to compare its effectiveness on otitis externa induced by S. aureus in male albino Wistar rats with that of cefazolin. The antimicrobial activity testing was conducted using the Kirby-Bauer disk diffusion method as described by the European Committee on Antimicrobial Susceptibility Testing. To induce otitis externa, we applied 0.5 ml of S. aureus to the ear skin using a hypodermic syringe (S. aureus strain TACK 25923 was dispersed on the 900x10-6 concentration of colony forming units per ml). Nimesulide and cefazolin were administered orally at a dose of 50 mg/kg. The antibacterial activity of cefazolin when used in equal doses (50 µg/ml) was more powerful against S. aureus than nimesulide. However, nimesulide reduced the macroscopic findings (such as oedema and redness) induced by S. aureus better than cefazolin. Additionally, nimesulide inhibited the increase of oxidant and proinflammatory indicators related to S. aureus, while cefazolin was able to inhibit only the increase in proinflammatory indicators. Our results showed that nimesulide was superior to cefazolin in terms of reducing the external ear damage caused by S. aureus. Therefore, nimesulide monotherapy or a combination of nimesulide and cefazolin therapy may be beneficial in the treatment of bacterial otitis externa. [Med-Science 2021; 10(2.000): 577-82] |
| first_indexed | 2024-03-08T06:42:03Z |
| format | Article |
| id | doaj.art-3bca903f990e4a8fbf3d89bec1e04d58 |
| institution | Directory Open Access Journal |
| issn | 2147-0634 |
| language | English |
| last_indexed | 2024-03-08T06:42:03Z |
| publishDate | 2021-06-01 |
| publisher | Society of Turaz Bilim |
| record_format | Article |
| series | Medicine Science |
| spelling | doaj.art-3bca903f990e4a8fbf3d89bec1e04d582024-02-03T08:37:00ZengSociety of Turaz BilimMedicine Science2147-06342021-06-011025778210.5455/medscience.2021.02.05057001Protective effect of nimesulide on the external ear damage induced by staphylococcus aureus inoculation in ratsErtugrul Erhan0Ismail Salcan1Muhammet Dilber2Sumeyye Akyuz3Bulent Dabanlioglu4Bahadir Suleyman5Taha Abdulkadir Coban6Halis Suleyman7Renad Mammadov8Department of Otorhinolaryngology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey. Department of Otorhinolaryngology, Mengucek Gazi Training and Research Hospital, Erzincan, Turkey. ENT Special Doctor, Istanbul, Turkey. Department of Microbiology, Mengucek Gazi Training and Research Hospital, Erzincan, Turkey. Department of Microbiology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey. Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey. Department of Biochemistry, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey. Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey. Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey.Since the external ear is covered with skin, infections that cause otitis externa are often produced by distinct skin flora in several different areas. Staphylococcus aureus (S. aureus) reproduces the most out of all bacteria isolated from external auditory canal skin culture samples. Proinflammatory cytokines are the main components responsible for tissue damage pathogenesis due to S. aureus. Nimesulide is a nonsteroidal anti-inflammatory drug that selectively inhibits cyclooxygenase-2 and also demonstrates antioxidant properties. The present study aimed to examine the antibacterial activity of nimesulide against S. aureus and to compare its effectiveness on otitis externa induced by S. aureus in male albino Wistar rats with that of cefazolin. The antimicrobial activity testing was conducted using the Kirby-Bauer disk diffusion method as described by the European Committee on Antimicrobial Susceptibility Testing. To induce otitis externa, we applied 0.5 ml of S. aureus to the ear skin using a hypodermic syringe (S. aureus strain TACK 25923 was dispersed on the 900x10-6 concentration of colony forming units per ml). Nimesulide and cefazolin were administered orally at a dose of 50 mg/kg. The antibacterial activity of cefazolin when used in equal doses (50 µg/ml) was more powerful against S. aureus than nimesulide. However, nimesulide reduced the macroscopic findings (such as oedema and redness) induced by S. aureus better than cefazolin. Additionally, nimesulide inhibited the increase of oxidant and proinflammatory indicators related to S. aureus, while cefazolin was able to inhibit only the increase in proinflammatory indicators. Our results showed that nimesulide was superior to cefazolin in terms of reducing the external ear damage caused by S. aureus. Therefore, nimesulide monotherapy or a combination of nimesulide and cefazolin therapy may be beneficial in the treatment of bacterial otitis externa. [Med-Science 2021; 10(2.000): 577-82]http://www.ejmanager.com/fulltextpdf.php?mno=57001nimesulidecefazolinstaphylococcus aureusear damagerat |
| spellingShingle | Ertugrul Erhan Ismail Salcan Muhammet Dilber Sumeyye Akyuz Bulent Dabanlioglu Bahadir Suleyman Taha Abdulkadir Coban Halis Suleyman Renad Mammadov Protective effect of nimesulide on the external ear damage induced by staphylococcus aureus inoculation in rats Medicine Science nimesulide cefazolin staphylococcus aureus ear damage rat |
| title | Protective effect of nimesulide on the external ear damage induced by staphylococcus aureus inoculation in rats |
| title_full | Protective effect of nimesulide on the external ear damage induced by staphylococcus aureus inoculation in rats |
| title_fullStr | Protective effect of nimesulide on the external ear damage induced by staphylococcus aureus inoculation in rats |
| title_full_unstemmed | Protective effect of nimesulide on the external ear damage induced by staphylococcus aureus inoculation in rats |
| title_short | Protective effect of nimesulide on the external ear damage induced by staphylococcus aureus inoculation in rats |
| title_sort | protective effect of nimesulide on the external ear damage induced by staphylococcus aureus inoculation in rats |
| topic | nimesulide cefazolin staphylococcus aureus ear damage rat |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=57001 |
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