DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells.
Chromosome breakage is a major threat to genome integrity. The most accurate way to repair DNA double strand breaks (DSB) is homologous recombination (HR) with an intact copy of the broken locus. Mobility of the broken DNA has been seen to increase during the search for a donor copy. Observing chrom...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-03-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3952824?pdf=render |
| _version_ | 1811330515988381696 |
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| author | Hicham Saad Franck Gallardo Mathieu Dalvai Nicolas Tanguy-le-Gac David Lane Kerstin Bystricky |
| author_facet | Hicham Saad Franck Gallardo Mathieu Dalvai Nicolas Tanguy-le-Gac David Lane Kerstin Bystricky |
| author_sort | Hicham Saad |
| collection | DOAJ |
| description | Chromosome breakage is a major threat to genome integrity. The most accurate way to repair DNA double strand breaks (DSB) is homologous recombination (HR) with an intact copy of the broken locus. Mobility of the broken DNA has been seen to increase during the search for a donor copy. Observing chromosome dynamics during the earlier steps of HR, mainly the resection from DSB ends that generates recombinogenic single strands, requires a visualization system that does not interfere with the process, and is small relative to the few kilobases of DNA that undergo processing. Current visualization tools, based on binding of fluorescent repressor proteins to arrays of specific binding sites, have the major drawback that highly-repeated DNA and lengthy stretches of strongly bound protein can obstruct chromatin function. We have developed a new, non-intrusive method which uses protein oligomerization rather than operator multiplicity to form visible foci. By applying it to HO cleavage of the MAT locus on Saccharomyces cerevisiae chromosome III, we provide the first real-time analysis of resection in single living cells. Monitoring the dynamics of a chromatin locus next to a DSB revealed transient confinement of the damaged chromatin region during the very early steps of resection, consistent with the need to keep DNA ends in contact. Resection in a yku70 mutant began ∼ 10 min earlier than in wild type, defining this as the period of commitment to homology-dependent repair. Beyond the insights into the dynamics and mechanism of resection, our new DNA-labelling and -targeting method will be widely applicable to fine-scale analysis of genome organization, dynamics and function in normal and pathological contexts. |
| first_indexed | 2024-04-13T16:04:21Z |
| format | Article |
| id | doaj.art-3bcfe8119b74426ca2f419e6c91a1213 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T16:04:21Z |
| publishDate | 2014-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-3bcfe8119b74426ca2f419e6c91a12132022-12-22T02:40:27ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042014-03-01103e100418710.1371/journal.pgen.1004187DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells.Hicham SaadFranck GallardoMathieu DalvaiNicolas Tanguy-le-GacDavid LaneKerstin BystrickyChromosome breakage is a major threat to genome integrity. The most accurate way to repair DNA double strand breaks (DSB) is homologous recombination (HR) with an intact copy of the broken locus. Mobility of the broken DNA has been seen to increase during the search for a donor copy. Observing chromosome dynamics during the earlier steps of HR, mainly the resection from DSB ends that generates recombinogenic single strands, requires a visualization system that does not interfere with the process, and is small relative to the few kilobases of DNA that undergo processing. Current visualization tools, based on binding of fluorescent repressor proteins to arrays of specific binding sites, have the major drawback that highly-repeated DNA and lengthy stretches of strongly bound protein can obstruct chromatin function. We have developed a new, non-intrusive method which uses protein oligomerization rather than operator multiplicity to form visible foci. By applying it to HO cleavage of the MAT locus on Saccharomyces cerevisiae chromosome III, we provide the first real-time analysis of resection in single living cells. Monitoring the dynamics of a chromatin locus next to a DSB revealed transient confinement of the damaged chromatin region during the very early steps of resection, consistent with the need to keep DNA ends in contact. Resection in a yku70 mutant began ∼ 10 min earlier than in wild type, defining this as the period of commitment to homology-dependent repair. Beyond the insights into the dynamics and mechanism of resection, our new DNA-labelling and -targeting method will be widely applicable to fine-scale analysis of genome organization, dynamics and function in normal and pathological contexts.http://europepmc.org/articles/PMC3952824?pdf=render |
| spellingShingle | Hicham Saad Franck Gallardo Mathieu Dalvai Nicolas Tanguy-le-Gac David Lane Kerstin Bystricky DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. PLoS Genetics |
| title | DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. |
| title_full | DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. |
| title_fullStr | DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. |
| title_full_unstemmed | DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. |
| title_short | DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. |
| title_sort | dna dynamics during early double strand break processing revealed by non intrusive imaging of living cells |
| url | http://europepmc.org/articles/PMC3952824?pdf=render |
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