Immunohistochemical and molecular detection of mannheimia spp. and pasteurella spp. in sheep with pneumonia in kars province (turkey)
In this investigation, presence of Mannheimia haemolytica and Pasteurella multocida in sheep brought to Kafkas University Faculty of Veterinary Medicine between 2011 and 2013 with a suspicion of pneumonia was studied by immunohistochemical and bacteriological methods. Study materials were 100 sheep...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2018-01-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2320 |
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| author | Serpil DAĞ Abdurrahman GÜRBÜZ Hasan ÖZEN Fatih BÜYÜK Özgür ÇELEBİ Musa KARAMAN Mehmet ÇİTİL Emin KARAKURT |
| author_facet | Serpil DAĞ Abdurrahman GÜRBÜZ Hasan ÖZEN Fatih BÜYÜK Özgür ÇELEBİ Musa KARAMAN Mehmet ÇİTİL Emin KARAKURT |
| author_sort | Serpil DAĞ |
| collection | DOAJ |
| description | In this investigation, presence of Mannheimia haemolytica and Pasteurella multocida in sheep brought to Kafkas University Faculty of Veterinary
Medicine between 2011 and 2013 with a suspicion of pneumonia was studied by immunohistochemical and bacteriological methods. Study materials
were 100 sheep lungs. After routine histopathological investigations lungs were immunohistochemically stained with antibodies prepared against
both of the agents. In bacteriological investigations, tissue samples were inoculated into 7% sheep blood agar and Mc Conkey agar and incubated for
24-48 hours in aerobic conditions, and the suspected colonies were evaluated for Mannheimia and Pasteurella species. In histological investigations,
pneumonias were classified as 28% fibrinous bronchopneumonia, 21% prulent bronchopneumonia, 20% acute-catharal bronchopneumonia, 18%
interstitial pneumonia, 11% verminous pneumonia and 2% adenomatous pneumonia. In bacteriological investigations Mannheimia haemolytica and
Pasteurella multocida was detected in 19 and 3 cases, respectively, these numbers were 17 and 2, respectively in immunohistochemical investigations.
The results of PCR analysis for both Mannheimia haemolytica and Pasteurella multocida paralleled with the findings of microbiological culture. The
results of the study showed that Mannheimia haemolytica is an important cause of pneumonia in sheep in Kars, and Pasteurella multocida, although
with lesser importance can cause pneumonia in this species. |
| first_indexed | 2024-03-13T04:37:09Z |
| format | Article |
| id | doaj.art-3bd0f9dc316d4d04b2fe37b0748eac5b |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:09Z |
| publishDate | 2018-01-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-3bd0f9dc316d4d04b2fe37b0748eac5b2023-06-19T06:44:09ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512018-01-0124228128810.9775/kvfd.2017.188402320Immunohistochemical and molecular detection of mannheimia spp. and pasteurella spp. in sheep with pneumonia in kars province (turkey)Serpil DAĞ0Abdurrahman GÜRBÜZ1Hasan ÖZEN2Fatih BÜYÜK3Özgür ÇELEBİ4Musa KARAMAN5Mehmet ÇİTİL6Emin KARAKURT7Kafkas University, Faculty of Veterinary Medicine, Department of Pathology, TR-36100 Kars - TURKEYKafkas University, Atatürk Health Services Vocational School, TR-36100 Kars - TURKEYKafkas University, Faculty of Veterinary Medicine, Department of Pathology, TR-36100 Kars - TURKEYKafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36100 Kars - TURKEYKafkas University, Faculty of Veterinary Medicine, Department of Microbiology, TR-36100 Kars - TURKEYBalıkesir University, Faculty of Veterinary Medicine, Department of Pathology, TR-10145 Balıkesir - TURKEYErciyes University, Faculty of Veterinary Medicine, Department of Internal Medicine, TR-38039 Kayseri - TURKEYKafkas University, Faculty of Veterinary Medicine, Department of Pathology, TR-36100 Kars - TURKEYIn this investigation, presence of Mannheimia haemolytica and Pasteurella multocida in sheep brought to Kafkas University Faculty of Veterinary Medicine between 2011 and 2013 with a suspicion of pneumonia was studied by immunohistochemical and bacteriological methods. Study materials were 100 sheep lungs. After routine histopathological investigations lungs were immunohistochemically stained with antibodies prepared against both of the agents. In bacteriological investigations, tissue samples were inoculated into 7% sheep blood agar and Mc Conkey agar and incubated for 24-48 hours in aerobic conditions, and the suspected colonies were evaluated for Mannheimia and Pasteurella species. In histological investigations, pneumonias were classified as 28% fibrinous bronchopneumonia, 21% prulent bronchopneumonia, 20% acute-catharal bronchopneumonia, 18% interstitial pneumonia, 11% verminous pneumonia and 2% adenomatous pneumonia. In bacteriological investigations Mannheimia haemolytica and Pasteurella multocida was detected in 19 and 3 cases, respectively, these numbers were 17 and 2, respectively in immunohistochemical investigations. The results of PCR analysis for both Mannheimia haemolytica and Pasteurella multocida paralleled with the findings of microbiological culture. The results of the study showed that Mannheimia haemolytica is an important cause of pneumonia in sheep in Kars, and Pasteurella multocida, although with lesser importance can cause pneumonia in this species.https://vetdergikafkas.org/pdf.php?id=2320mannheimia haemolyticapasteurella multocidapneumoniasheepimmunohistochemistry |
| spellingShingle | Serpil DAĞ Abdurrahman GÜRBÜZ Hasan ÖZEN Fatih BÜYÜK Özgür ÇELEBİ Musa KARAMAN Mehmet ÇİTİL Emin KARAKURT Immunohistochemical and molecular detection of mannheimia spp. and pasteurella spp. in sheep with pneumonia in kars province (turkey) Kafkas Universitesi Veteriner Fakültesi Dergisi mannheimia haemolytica pasteurella multocida pneumonia sheep immunohistochemistry |
| title | Immunohistochemical and molecular detection of mannheimia spp. and pasteurella spp. in sheep with pneumonia in kars province (turkey) |
| title_full | Immunohistochemical and molecular detection of mannheimia spp. and pasteurella spp. in sheep with pneumonia in kars province (turkey) |
| title_fullStr | Immunohistochemical and molecular detection of mannheimia spp. and pasteurella spp. in sheep with pneumonia in kars province (turkey) |
| title_full_unstemmed | Immunohistochemical and molecular detection of mannheimia spp. and pasteurella spp. in sheep with pneumonia in kars province (turkey) |
| title_short | Immunohistochemical and molecular detection of mannheimia spp. and pasteurella spp. in sheep with pneumonia in kars province (turkey) |
| title_sort | immunohistochemical and molecular detection of mannheimia spp and pasteurella spp in sheep with pneumonia in kars province turkey |
| topic | mannheimia haemolytica pasteurella multocida pneumonia sheep immunohistochemistry |
| url | https://vetdergikafkas.org/pdf.php?id=2320 |
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