Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device
Carcinoembryonic antigen (CEA) is a broad-spectrum tumor marker used in clinical applications. The primarily clinical method for measuring CEA is based on chemiluminescence in serum during enzyme-linked immunosorbent assays (ELISA) in 96-well plates. However, this multi-step process requires large a...
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MDPI AG
2018-09-01
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| Online Access: | http://www.mdpi.com/2072-666X/9/9/470 |
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| author | Zhigang Gao Zongzheng Chen Jiu Deng Xiaorui Li Yueyang Qu Lingling Xu Yong Luo Yao Lu Tingjiao Liu Weijie Zhao Bingcheng Lin |
| author_facet | Zhigang Gao Zongzheng Chen Jiu Deng Xiaorui Li Yueyang Qu Lingling Xu Yong Luo Yao Lu Tingjiao Liu Weijie Zhao Bingcheng Lin |
| author_sort | Zhigang Gao |
| collection | DOAJ |
| description | Carcinoembryonic antigen (CEA) is a broad-spectrum tumor marker used in clinical applications. The primarily clinical method for measuring CEA is based on chemiluminescence in serum during enzyme-linked immunosorbent assays (ELISA) in 96-well plates. However, this multi-step process requires large and expensive instruments, and takes a long time. In this study, a high-throughput centrifugal microfluidic device was developed for detecting CEA in serum without the need for cumbersome washing steps normally used in immunoreactions. This centrifugal microdevice contains 14 identical pencil-like units, and the CEA molecules are separated from the bulk serum for subsequent immunofluorescence detection using density gradient centrifugation in each unit simultaneously. To determine the optimal conditions for CEA detection in serum, the effects of the density of the medium, rotation speed, and spin duration were investigated. The measured values from 34 clinical serum samples using this high-throughput centrifugal microfluidic device showed good agreement with the known values (average relative error = 9.22%). These results indicate that the high-throughput centrifugal microfluidic device could provide an alternative approach for replacing the classical method for CEA detection in clinical serum samples. |
| first_indexed | 2024-12-14T12:20:36Z |
| format | Article |
| id | doaj.art-3bd3081828b449d19ebec6fa7521736d |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-14T12:20:36Z |
| publishDate | 2018-09-01 |
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| series | Micromachines |
| spelling | doaj.art-3bd3081828b449d19ebec6fa7521736d2022-12-21T23:01:29ZengMDPI AGMicromachines2072-666X2018-09-019947010.3390/mi9090470mi9090470Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic DeviceZhigang Gao0Zongzheng Chen1Jiu Deng2Xiaorui Li3Yueyang Qu4Lingling Xu5Yong Luo6Yao Lu7Tingjiao Liu8Weijie Zhao9Bingcheng Lin10School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, ChinaIntegrated Chinese and Western Medicine Postdoctoral research station, Jinan University, Guangzhou 510632, ChinaSchool of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, ChinaSchool of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, ChinaSchool of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, ChinaSchool of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, ChinaSchool of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, ChinaDalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaCollege of Stomatology, Dalian Medical University, Dalian 116024, ChinaSchool of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, ChinaSchool of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, ChinaCarcinoembryonic antigen (CEA) is a broad-spectrum tumor marker used in clinical applications. The primarily clinical method for measuring CEA is based on chemiluminescence in serum during enzyme-linked immunosorbent assays (ELISA) in 96-well plates. However, this multi-step process requires large and expensive instruments, and takes a long time. In this study, a high-throughput centrifugal microfluidic device was developed for detecting CEA in serum without the need for cumbersome washing steps normally used in immunoreactions. This centrifugal microdevice contains 14 identical pencil-like units, and the CEA molecules are separated from the bulk serum for subsequent immunofluorescence detection using density gradient centrifugation in each unit simultaneously. To determine the optimal conditions for CEA detection in serum, the effects of the density of the medium, rotation speed, and spin duration were investigated. The measured values from 34 clinical serum samples using this high-throughput centrifugal microfluidic device showed good agreement with the known values (average relative error = 9.22%). These results indicate that the high-throughput centrifugal microfluidic device could provide an alternative approach for replacing the classical method for CEA detection in clinical serum samples.http://www.mdpi.com/2072-666X/9/9/470centrifugal microfluidic deviceCEA detectiondensity mediumfluorescent chemiluminescence |
| spellingShingle | Zhigang Gao Zongzheng Chen Jiu Deng Xiaorui Li Yueyang Qu Lingling Xu Yong Luo Yao Lu Tingjiao Liu Weijie Zhao Bingcheng Lin Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device Micromachines centrifugal microfluidic device CEA detection density medium fluorescent chemiluminescence |
| title | Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device |
| title_full | Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device |
| title_fullStr | Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device |
| title_full_unstemmed | Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device |
| title_short | Measurement of Carcinoembryonic Antigen in Clinical Serum Samples Using a Centrifugal Microfluidic Device |
| title_sort | measurement of carcinoembryonic antigen in clinical serum samples using a centrifugal microfluidic device |
| topic | centrifugal microfluidic device CEA detection density medium fluorescent chemiluminescence |
| url | http://www.mdpi.com/2072-666X/9/9/470 |
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