Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers
Abstract The polyphenol content and antioxidant capacity of hyperforin and hypericin-standardized H. perforatum L. extracts may vary due to the harvest time. In this work, ethanol and ethanol–water extracts of air-dried and lyophilized flowers of H. perforatum L., collected throughout a vegetation s...
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| Format: | Article |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-83409-4 |
| _version_ | 1818752003564634112 |
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| author | Katerina Makarova Joanna J. Sajkowska-Kozielewicz Katarzyna Zawada Ewa Olchowik-Grabarek Michał Aleksander Ciach Krzysztof Gogolewski Natalia Dobros Paulina Ciechowicz Hélène Freichels Anna Gambin |
| author_facet | Katerina Makarova Joanna J. Sajkowska-Kozielewicz Katarzyna Zawada Ewa Olchowik-Grabarek Michał Aleksander Ciach Krzysztof Gogolewski Natalia Dobros Paulina Ciechowicz Hélène Freichels Anna Gambin |
| author_sort | Katerina Makarova |
| collection | DOAJ |
| description | Abstract The polyphenol content and antioxidant capacity of hyperforin and hypericin-standardized H. perforatum L. extracts may vary due to the harvest time. In this work, ethanol and ethanol–water extracts of air-dried and lyophilized flowers of H. perforatum L., collected throughout a vegetation season in central Poland, were studied. Air-dried flowers extracts had higher polyphenol (371 mg GAE/g) and flavonoid (160 mg CAE/g) content, DPPH radical scavenging (1672 mg DPPH/g), ORAC (5214 µmol TE/g) and FRAP (2.54 mmol Fe2+/g) than lyophilized flowers extracts (238 mg GAE/g, 107 mg CAE/g, 1287 mg DPPH/g, 3313 µmol TE/g and 0.31 mmol Fe2+/g, respectively). Principal component analysis showed that the collection date influenced the flavonoid and polyphenol contents and FRAP of ethanol extracts, and DPPH and ORAC values of ethanol–water extracts. The ethanol extracts with the highest polyphenol and flavonoid content protected human erythrocytes against bisphenol A-induced damage. Both high field and benchtop NMR spectra of selected extracts, revealed differences in composition caused by extraction solvent and raw material collection date. Moreover, we have shown that benchtop NMR can be used to detect the compositional variation of extracts if the assignment of signals is done previously. |
| first_indexed | 2024-12-18T04:44:33Z |
| format | Article |
| id | doaj.art-3bd3fc227b974e16b27f5e1d0dafe462 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:44:33Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3bd3fc227b974e16b27f5e1d0dafe4622022-12-21T21:20:36ZengNature PortfolioScientific Reports2045-23222021-02-0111111210.1038/s41598-021-83409-4Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowersKaterina Makarova0Joanna J. Sajkowska-Kozielewicz1Katarzyna Zawada2Ewa Olchowik-Grabarek3Michał Aleksander Ciach4Krzysztof Gogolewski5Natalia Dobros6Paulina Ciechowicz7Hélène Freichels8Anna Gambin9Department of Physical Chemistry, Chair of Physical Pharmacy and Bioanalysis, Faculty of Pharmacy With Laboratory Medicine Division, Medical University of WarsawDepartment of Physical Chemistry, Chair of Physical Pharmacy and Bioanalysis, Faculty of Pharmacy With Laboratory Medicine Division, Medical University of WarsawDepartment of Physical Chemistry, Chair of Physical Pharmacy and Bioanalysis, Faculty of Pharmacy With Laboratory Medicine Division, Medical University of WarsawLaboratory of Molecular Biophysics, Department of Microbiology and Biotechnology, Faculty of Biology, University of BialystokFaculty of Mathematics, Informatics and Mechanics, University of WarsawFaculty of Mathematics, Informatics and Mechanics, University of WarsawDepartment of Physical Chemistry, Chair of Physical Pharmacy and Bioanalysis, Faculty of Pharmacy With Laboratory Medicine Division, Medical University of WarsawDepartment of Physical Chemistry, Chair of Physical Pharmacy and Bioanalysis, Faculty of Pharmacy With Laboratory Medicine Division, Medical University of WarsawMagritek GmbHFaculty of Mathematics, Informatics and Mechanics, University of WarsawAbstract The polyphenol content and antioxidant capacity of hyperforin and hypericin-standardized H. perforatum L. extracts may vary due to the harvest time. In this work, ethanol and ethanol–water extracts of air-dried and lyophilized flowers of H. perforatum L., collected throughout a vegetation season in central Poland, were studied. Air-dried flowers extracts had higher polyphenol (371 mg GAE/g) and flavonoid (160 mg CAE/g) content, DPPH radical scavenging (1672 mg DPPH/g), ORAC (5214 µmol TE/g) and FRAP (2.54 mmol Fe2+/g) than lyophilized flowers extracts (238 mg GAE/g, 107 mg CAE/g, 1287 mg DPPH/g, 3313 µmol TE/g and 0.31 mmol Fe2+/g, respectively). Principal component analysis showed that the collection date influenced the flavonoid and polyphenol contents and FRAP of ethanol extracts, and DPPH and ORAC values of ethanol–water extracts. The ethanol extracts with the highest polyphenol and flavonoid content protected human erythrocytes against bisphenol A-induced damage. Both high field and benchtop NMR spectra of selected extracts, revealed differences in composition caused by extraction solvent and raw material collection date. Moreover, we have shown that benchtop NMR can be used to detect the compositional variation of extracts if the assignment of signals is done previously.https://doi.org/10.1038/s41598-021-83409-4 |
| spellingShingle | Katerina Makarova Joanna J. Sajkowska-Kozielewicz Katarzyna Zawada Ewa Olchowik-Grabarek Michał Aleksander Ciach Krzysztof Gogolewski Natalia Dobros Paulina Ciechowicz Hélène Freichels Anna Gambin Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers Scientific Reports |
| title | Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers |
| title_full | Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers |
| title_fullStr | Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers |
| title_full_unstemmed | Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers |
| title_short | Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers |
| title_sort | harvest time affects antioxidant capacity total polyphenol and flavonoid content of polish st john s wort s hypericum perforatum l flowers |
| url | https://doi.org/10.1038/s41598-021-83409-4 |
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