Characterization of diamine oxidase-based biosensors for biogenic amines detection assembled onto functionalized SiO₂ substrates using aliphatic and aromatic di-aldehydes as crosslinkers
ABSTRACTDevelopment of two biosensors to detect biogenic amines was carried out using immobilized diamine oxidase from Pisum sativum on 3-APTMS-functionalized SiO2 substrates with either glutaraldehyde or terephthalaldehyde as crosslinkers. The assembly process was validated by FT-IR, SEM-EDX, and A...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
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| Series: | CyTA - Journal of Food |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2023.2256820 |
| _version_ | 1827585505142767616 |
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| author | Luis Ramiro Caso Vargas Norma Angélica Caballero Concha Leslie Susana Arcila Lozano Raúl Delgado-Macuil Efraín Rubio-Rosas Alma Delia Luna González Melisa Carmona López Andrea Lizbeth Cortés Noriega Xquenda Ríos Aguilar Erick Saúl González Méndez |
| author_facet | Luis Ramiro Caso Vargas Norma Angélica Caballero Concha Leslie Susana Arcila Lozano Raúl Delgado-Macuil Efraín Rubio-Rosas Alma Delia Luna González Melisa Carmona López Andrea Lizbeth Cortés Noriega Xquenda Ríos Aguilar Erick Saúl González Méndez |
| author_sort | Luis Ramiro Caso Vargas |
| collection | DOAJ |
| description | ABSTRACTDevelopment of two biosensors to detect biogenic amines was carried out using immobilized diamine oxidase from Pisum sativum on 3-APTMS-functionalized SiO2 substrates with either glutaraldehyde or terephthalaldehyde as crosslinkers. The assembly process was validated by FT-IR, SEM-EDX, and AFM. Biosensor responses to biogenic amines were c.a. 3-fold lower compared to the free enzyme kinetics as assessed by UV/VIS spectrophotometry. The calculated initial velocities (ΔAbs·min-1) for glutaraldehyde and terephthalaldehyde biosensors in the given order were (3.2 ± 0.68) × 10–2, (0.48 ± 0.09) × 10–2 for putrescine; (4.6 ± 1.2) × 10–2, (0.35 ± 0.47) × 10–2 for cadaverine; (5.8 ± 2.4) × 10–3, (19.0 ± 155) × 10–3 for spermidine, and (3.0 ± 2.0) × 10–4, (0.0 ± 7) × 10–4for histamine. The 2-way t-tests indicated that the crosslinker type affected the catalytic activity of immobilized diamine oxidase as the responses of glutaraldehyde biosensors to putrescine and cadaverine were significantly higher than those of the terephthalaldehyde. Molecular Docking simulations showed that glutaraldehyde has a stronger affinity for DAO’s lysine and arginine residues while terephthalaldehyde to phenylalanine. |
| first_indexed | 2024-03-08T23:47:28Z |
| format | Article |
| id | doaj.art-3bd62005e5a04825a4d3778a7531323d |
| institution | Directory Open Access Journal |
| issn | 1947-6337 1947-6345 |
| language | English |
| last_indexed | 2024-03-08T23:47:28Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj.art-3bd62005e5a04825a4d3778a7531323d2023-12-13T14:31:47ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452023-12-0121157057910.1080/19476337.2023.2256820Characterization of diamine oxidase-based biosensors for biogenic amines detection assembled onto functionalized SiO₂ substrates using aliphatic and aromatic di-aldehydes as crosslinkersLuis Ramiro Caso Vargas0Norma Angélica Caballero Concha1Leslie Susana Arcila Lozano2Raúl Delgado-Macuil3Efraín Rubio-Rosas4Alma Delia Luna González5Melisa Carmona López6Andrea Lizbeth Cortés Noriega7Xquenda Ríos Aguilar8Erick Saúl González Méndez9Biotecnología – Facultad de Ciencias Biológicas, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoBiotecnología – Facultad de Ciencias Biológicas, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoConsejo Nacional de Humanidades, Ciencias y Tecnologías – IPN-CIBA, Tepetitla, MexicoCaracterización de Materiales y Biosensores, Centro de Investigación en Biotecnología Aplicada – IPN, Tepetitla, MexicoDirección de Innovación y Transferencia del Conocimiento, Centro Universitario de Vinculación y Transferencia de Tecnología, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoBiotecnología – Facultad de Ciencias Biológicas, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoBiotecnología – Facultad de Ciencias Biológicas, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoBiotecnología – Facultad de Ciencias Biológicas, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoBiotecnología – Facultad de Ciencias Biológicas, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoBiotecnología – Facultad de Ciencias Biológicas, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoABSTRACTDevelopment of two biosensors to detect biogenic amines was carried out using immobilized diamine oxidase from Pisum sativum on 3-APTMS-functionalized SiO2 substrates with either glutaraldehyde or terephthalaldehyde as crosslinkers. The assembly process was validated by FT-IR, SEM-EDX, and AFM. Biosensor responses to biogenic amines were c.a. 3-fold lower compared to the free enzyme kinetics as assessed by UV/VIS spectrophotometry. The calculated initial velocities (ΔAbs·min-1) for glutaraldehyde and terephthalaldehyde biosensors in the given order were (3.2 ± 0.68) × 10–2, (0.48 ± 0.09) × 10–2 for putrescine; (4.6 ± 1.2) × 10–2, (0.35 ± 0.47) × 10–2 for cadaverine; (5.8 ± 2.4) × 10–3, (19.0 ± 155) × 10–3 for spermidine, and (3.0 ± 2.0) × 10–4, (0.0 ± 7) × 10–4for histamine. The 2-way t-tests indicated that the crosslinker type affected the catalytic activity of immobilized diamine oxidase as the responses of glutaraldehyde biosensors to putrescine and cadaverine were significantly higher than those of the terephthalaldehyde. Molecular Docking simulations showed that glutaraldehyde has a stronger affinity for DAO’s lysine and arginine residues while terephthalaldehyde to phenylalanine.https://www.tandfonline.com/doi/10.1080/19476337.2023.2256820Biosensorsbiogenic aminesdiamine oxidasecrosslinkers |
| spellingShingle | Luis Ramiro Caso Vargas Norma Angélica Caballero Concha Leslie Susana Arcila Lozano Raúl Delgado-Macuil Efraín Rubio-Rosas Alma Delia Luna González Melisa Carmona López Andrea Lizbeth Cortés Noriega Xquenda Ríos Aguilar Erick Saúl González Méndez Characterization of diamine oxidase-based biosensors for biogenic amines detection assembled onto functionalized SiO₂ substrates using aliphatic and aromatic di-aldehydes as crosslinkers CyTA - Journal of Food Biosensors biogenic amines diamine oxidase crosslinkers |
| title | Characterization of diamine oxidase-based biosensors for biogenic amines detection assembled onto functionalized SiO₂ substrates using aliphatic and aromatic di-aldehydes as crosslinkers |
| title_full | Characterization of diamine oxidase-based biosensors for biogenic amines detection assembled onto functionalized SiO₂ substrates using aliphatic and aromatic di-aldehydes as crosslinkers |
| title_fullStr | Characterization of diamine oxidase-based biosensors for biogenic amines detection assembled onto functionalized SiO₂ substrates using aliphatic and aromatic di-aldehydes as crosslinkers |
| title_full_unstemmed | Characterization of diamine oxidase-based biosensors for biogenic amines detection assembled onto functionalized SiO₂ substrates using aliphatic and aromatic di-aldehydes as crosslinkers |
| title_short | Characterization of diamine oxidase-based biosensors for biogenic amines detection assembled onto functionalized SiO₂ substrates using aliphatic and aromatic di-aldehydes as crosslinkers |
| title_sort | characterization of diamine oxidase based biosensors for biogenic amines detection assembled onto functionalized sio₂ substrates using aliphatic and aromatic di aldehydes as crosslinkers |
| topic | Biosensors biogenic amines diamine oxidase crosslinkers |
| url | https://www.tandfonline.com/doi/10.1080/19476337.2023.2256820 |
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