The <i>Sl</i>SWEET12c Sugar Transporter Promotes Sucrose Unloading and Metabolism in Ripening Tomato Fruits
Sugar content is a primary determinant of taste and quality in tomato (<i>Solanum lycopersicum</i>) fruit. Sugar allocation from source to sink is dependent on the activity of plasma membrane sugar transporters and is a critical process in plant development. Sugar will eventually be expo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/10/935 |
| _version_ | 1797472965827231744 |
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| author | Jiaqi Sun Chaoyang Feng Xin Liu Jing Jiang |
| author_facet | Jiaqi Sun Chaoyang Feng Xin Liu Jing Jiang |
| author_sort | Jiaqi Sun |
| collection | DOAJ |
| description | Sugar content is a primary determinant of taste and quality in tomato (<i>Solanum lycopersicum</i>) fruit. Sugar allocation from source to sink is dependent on the activity of plasma membrane sugar transporters and is a critical process in plant development. Sugar will eventually be exported transporters (SWEETs) are sugar transporters that play key roles in plant biology, including growth and development. However, few studies have been conducted on the tomato SWEET protein family to date. Through gene expression analysis, we found that <i>SlSWEET12c</i> had the highest expression during the red ripening stage of tomato fruits. Yeast functional complementation, subcellular localization, and GUS activity assays showed that <i>Sl</i>SWEET12c is a plasma membrane-localized sugar transporter that accumulates in the vascular bundles, carpel, and sarcocarp. Silencing <i>SlSWEET12c</i> increased sucrose accumulation and reduced the number of hexoses in tomato fruits; the opposite effects were observed under <i>SISWEET12c</i> overexpression. Invertase activity was also decreased after silencing <i>SISWEET12c</i>. These results suggest that <i>SlSWEET12c</i> is a sugar transporter that promotes sucrose unloading and metabolism in ripening tomato fruits, offering a new target for improving tomato quality and production. |
| first_indexed | 2024-03-09T20:08:32Z |
| format | Article |
| id | doaj.art-3bd97c7666734a8da716975e9a4573c5 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T20:08:32Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-3bd97c7666734a8da716975e9a4573c52023-11-24T00:23:36ZengMDPI AGHorticulturae2311-75242022-10-0181093510.3390/horticulturae8100935The <i>Sl</i>SWEET12c Sugar Transporter Promotes Sucrose Unloading and Metabolism in Ripening Tomato FruitsJiaqi Sun0Chaoyang Feng1Xin Liu2Jing Jiang3College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Biology and Agriculture, Zunyi Normal College, Zunyi 563002, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaSugar content is a primary determinant of taste and quality in tomato (<i>Solanum lycopersicum</i>) fruit. Sugar allocation from source to sink is dependent on the activity of plasma membrane sugar transporters and is a critical process in plant development. Sugar will eventually be exported transporters (SWEETs) are sugar transporters that play key roles in plant biology, including growth and development. However, few studies have been conducted on the tomato SWEET protein family to date. Through gene expression analysis, we found that <i>SlSWEET12c</i> had the highest expression during the red ripening stage of tomato fruits. Yeast functional complementation, subcellular localization, and GUS activity assays showed that <i>Sl</i>SWEET12c is a plasma membrane-localized sugar transporter that accumulates in the vascular bundles, carpel, and sarcocarp. Silencing <i>SlSWEET12c</i> increased sucrose accumulation and reduced the number of hexoses in tomato fruits; the opposite effects were observed under <i>SISWEET12c</i> overexpression. Invertase activity was also decreased after silencing <i>SISWEET12c</i>. These results suggest that <i>SlSWEET12c</i> is a sugar transporter that promotes sucrose unloading and metabolism in ripening tomato fruits, offering a new target for improving tomato quality and production.https://www.mdpi.com/2311-7524/8/10/935SWEETtomatosugar transportred ripening fruitsource–sinkinvertase |
| spellingShingle | Jiaqi Sun Chaoyang Feng Xin Liu Jing Jiang The <i>Sl</i>SWEET12c Sugar Transporter Promotes Sucrose Unloading and Metabolism in Ripening Tomato Fruits Horticulturae SWEET tomato sugar transport red ripening fruit source–sink invertase |
| title | The <i>Sl</i>SWEET12c Sugar Transporter Promotes Sucrose Unloading and Metabolism in Ripening Tomato Fruits |
| title_full | The <i>Sl</i>SWEET12c Sugar Transporter Promotes Sucrose Unloading and Metabolism in Ripening Tomato Fruits |
| title_fullStr | The <i>Sl</i>SWEET12c Sugar Transporter Promotes Sucrose Unloading and Metabolism in Ripening Tomato Fruits |
| title_full_unstemmed | The <i>Sl</i>SWEET12c Sugar Transporter Promotes Sucrose Unloading and Metabolism in Ripening Tomato Fruits |
| title_short | The <i>Sl</i>SWEET12c Sugar Transporter Promotes Sucrose Unloading and Metabolism in Ripening Tomato Fruits |
| title_sort | i sl i sweet12c sugar transporter promotes sucrose unloading and metabolism in ripening tomato fruits |
| topic | SWEET tomato sugar transport red ripening fruit source–sink invertase |
| url | https://www.mdpi.com/2311-7524/8/10/935 |
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