| Summary: | With the increasing antibiotic resistance of bacterial strains, alternative methods for infection control are in high demand. <i>Quorum sensing</i> (QS) is the bacterial communication system based on small molecules. QS is enables bacterial biofilm formation and pathogenic development. The interruption of QS has become a target for drug discovery, but remains in the early experimental phase. In this study, we synthesized a set of six compounds based on a scaffold (alkyl-quinoxalin-2(1<i>H</i>)-one), new in the anti-QS of Gram-negative bacteria <i>Aeromonas caviae</i> Sch3. By quantifying biofilm formation, we were able to monitor the effect of these compounds from concentrations of 1 to 100 µM. Significant reduction in biofilm formation was achieved by 3-hexylylquinoxalin-2(1<i>H</i>)-one (<b>11</b>), 3-hexylylquinoxalin-2(1<i>H</i>)-one-6-carboxylic acid (<b>12</b>), and 3-heptylylquinoxalin-2(1<i>H</i>)-one-6-carboxylic acid (<b>14</b>), ranging from 11% to 59% inhibition of the biofilm. This pilot study contributes to the development of anti-QS compounds to overcome the clinical challenge of resistant bacteria strains.
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