Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis
Characterization of the pollen allergens and identification of the main T-cell and B-cell epitopes is of crucial importance, since the clarification of the cellular and molecular mechanisms will allow long-term control of this type allergic diseases and development of safe immunotherapeutic medicati...
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| Format: | Article |
| Language: | English |
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Plovdiv University Press
2015-12-01
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| Series: | Journal of BioScience and Biotechnology |
| Subjects: | |
| Online Access: | http://www.jbb.uni-plovdiv.bg/documents/27807/1014563/jbb_2015-4(3)-pages_271-283.pdf |
| _version_ | 1828737603862528000 |
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| author | ZHIVKA KOSTOVA TSVETELINA BATSALOVA DZHEMAL MOTEN IVANKA TENEVA BALIK DZHAMBAZOV |
| author_facet | ZHIVKA KOSTOVA TSVETELINA BATSALOVA DZHEMAL MOTEN IVANKA TENEVA BALIK DZHAMBAZOV |
| author_sort | ZHIVKA KOSTOVA |
| collection | DOAJ |
| description | Characterization of the pollen allergens and identification of the main T-cell and B-cell epitopes is of crucial importance, since the clarification of the cellular and molecular mechanisms will allow long-term control of this type allergic diseases and development of safe immunotherapeutic medication. The objective of this study was to identify which pollen allergens are responsible for the T-cell activation in atopic patients with pollinosis and to determine whether the same allergens are responsible for the IgE-mediated reactions. Thirty-seven patients with pollinosis and thirteen non-allergic subjects were recruited. Peripheral blood samples were collected out of the pollen season. T-cell responses (IFN-γ production) towards different pollen allergens and levels of pollen specific IgE and IgG in the sera were measured by ELISA. The T-cell reactivity in most patients was directed towards the grass pollen B1, tree pollen I and autumn pollen B5. 70% of the allergic individuals responded to allergens from Lolium perenne, 84% to Dactylis glomerata, 11% to Phleum pratense, 65% to Betula pendula and 70% to Taxus baccata. Elevated serum levels of specific IgE in the allergic patients (p<0.01) were measured against the tree pollen I and autumn pollen, which include widespread deciduous trees (birch, willow, poplar and yew) and Artemisia absinthium, respectively. Our results show that the T-cell reactivity and antibody responses may be directed towards different or the same allergens. In addition, we propose that short synthetic peptides, which contain overlapping T- and B-cell epitopes can be used for specific immunotherapy treatment if they lack antibody recognition domains. |
| first_indexed | 2024-04-12T23:46:48Z |
| format | Article |
| id | doaj.art-3bdde522b83347e7b420d3f1069dfa63 |
| institution | Directory Open Access Journal |
| issn | 1314-6238 1314-6246 |
| language | English |
| last_indexed | 2024-04-12T23:46:48Z |
| publishDate | 2015-12-01 |
| publisher | Plovdiv University Press |
| record_format | Article |
| series | Journal of BioScience and Biotechnology |
| spelling | doaj.art-3bdde522b83347e7b420d3f1069dfa632022-12-22T03:11:50ZengPlovdiv University PressJournal of BioScience and Biotechnology1314-62381314-62462015-12-0143271283Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosisZHIVKA KOSTOVA0TSVETELINA BATSALOVA1DZHEMAL MOTEN2IVANKA TENEVA3BALIK DZHAMBAZOV4Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria.Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria.Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria.Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria.Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria.Characterization of the pollen allergens and identification of the main T-cell and B-cell epitopes is of crucial importance, since the clarification of the cellular and molecular mechanisms will allow long-term control of this type allergic diseases and development of safe immunotherapeutic medication. The objective of this study was to identify which pollen allergens are responsible for the T-cell activation in atopic patients with pollinosis and to determine whether the same allergens are responsible for the IgE-mediated reactions. Thirty-seven patients with pollinosis and thirteen non-allergic subjects were recruited. Peripheral blood samples were collected out of the pollen season. T-cell responses (IFN-γ production) towards different pollen allergens and levels of pollen specific IgE and IgG in the sera were measured by ELISA. The T-cell reactivity in most patients was directed towards the grass pollen B1, tree pollen I and autumn pollen B5. 70% of the allergic individuals responded to allergens from Lolium perenne, 84% to Dactylis glomerata, 11% to Phleum pratense, 65% to Betula pendula and 70% to Taxus baccata. Elevated serum levels of specific IgE in the allergic patients (p<0.01) were measured against the tree pollen I and autumn pollen, which include widespread deciduous trees (birch, willow, poplar and yew) and Artemisia absinthium, respectively. Our results show that the T-cell reactivity and antibody responses may be directed towards different or the same allergens. In addition, we propose that short synthetic peptides, which contain overlapping T- and B-cell epitopes can be used for specific immunotherapy treatment if they lack antibody recognition domains.http://www.jbb.uni-plovdiv.bg/documents/27807/1014563/jbb_2015-4(3)-pages_271-283.pdfpollen allergyT-cell responsesB-cell responsesallergens |
| spellingShingle | ZHIVKA KOSTOVA TSVETELINA BATSALOVA DZHEMAL MOTEN IVANKA TENEVA BALIK DZHAMBAZOV Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis Journal of BioScience and Biotechnology pollen allergy T-cell responses B-cell responses allergens |
| title | Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis |
| title_full | Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis |
| title_fullStr | Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis |
| title_full_unstemmed | Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis |
| title_short | Characteristics of T-cell and B-cell immune respoonses to pollen allergens in Bulgarian patients with pollinosis |
| title_sort | characteristics of t cell and b cell immune respoonses to pollen allergens in bulgarian patients with pollinosis |
| topic | pollen allergy T-cell responses B-cell responses allergens |
| url | http://www.jbb.uni-plovdiv.bg/documents/27807/1014563/jbb_2015-4(3)-pages_271-283.pdf |
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