A highly specific phage defense system is a conserved feature of the Vibrio cholerae mobilome.
Vibrio cholerae-specific bacteriophages are common features of the microbial community during cholera infection in humans. Phages impose strong selective pressure that favors the expansion of phage-resistant strains over their vulnerable counterparts. The mechanisms allowing virulent V. cholerae str...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-06-01
|
| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5481146?pdf=render |
| _version_ | 1819104730526253056 |
|---|---|
| author | Brendan J O'Hara Zachary K Barth Amelia C McKitterick Kimberley D Seed |
| author_facet | Brendan J O'Hara Zachary K Barth Amelia C McKitterick Kimberley D Seed |
| author_sort | Brendan J O'Hara |
| collection | DOAJ |
| description | Vibrio cholerae-specific bacteriophages are common features of the microbial community during cholera infection in humans. Phages impose strong selective pressure that favors the expansion of phage-resistant strains over their vulnerable counterparts. The mechanisms allowing virulent V. cholerae strains to defend against the ubiquitous threat of predatory phages have not been established. Here, we show that V. cholerae PLEs (phage-inducible chromosomal island-like elements) are widespread genomic islands dedicated to phage defense. Analysis of V. cholerae isolates spanning a 60-year collection period identified five unique PLEs. Remarkably, we found that all PLEs (regardless of geographic or temporal origin) respond to infection by a myovirus called ICP1, the most prominent V. cholerae phage found in cholera patient stool samples from Bangladesh. We found that PLE activity reduces phage genome replication and accelerates cell lysis following ICP1 infection, killing infected host cells and preventing the production of progeny phage. PLEs are mobilized by ICP1 infection and can spread to neighboring cells such that protection from phage predation can be horizontally acquired. Our results reveal that PLEs are a persistent feature of the V. cholerae mobilome that are adapted to providing protection from a single predatory phage and advance our understanding of how phages influence pathogen evolution. |
| first_indexed | 2024-12-22T02:11:00Z |
| format | Article |
| id | doaj.art-3bde1306dd004f6eae6315acfebabb4c |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-22T02:11:00Z |
| publishDate | 2017-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-3bde1306dd004f6eae6315acfebabb4c2022-12-21T18:42:24ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042017-06-01136e100683810.1371/journal.pgen.1006838A highly specific phage defense system is a conserved feature of the Vibrio cholerae mobilome.Brendan J O'HaraZachary K BarthAmelia C McKitterickKimberley D SeedVibrio cholerae-specific bacteriophages are common features of the microbial community during cholera infection in humans. Phages impose strong selective pressure that favors the expansion of phage-resistant strains over their vulnerable counterparts. The mechanisms allowing virulent V. cholerae strains to defend against the ubiquitous threat of predatory phages have not been established. Here, we show that V. cholerae PLEs (phage-inducible chromosomal island-like elements) are widespread genomic islands dedicated to phage defense. Analysis of V. cholerae isolates spanning a 60-year collection period identified five unique PLEs. Remarkably, we found that all PLEs (regardless of geographic or temporal origin) respond to infection by a myovirus called ICP1, the most prominent V. cholerae phage found in cholera patient stool samples from Bangladesh. We found that PLE activity reduces phage genome replication and accelerates cell lysis following ICP1 infection, killing infected host cells and preventing the production of progeny phage. PLEs are mobilized by ICP1 infection and can spread to neighboring cells such that protection from phage predation can be horizontally acquired. Our results reveal that PLEs are a persistent feature of the V. cholerae mobilome that are adapted to providing protection from a single predatory phage and advance our understanding of how phages influence pathogen evolution.http://europepmc.org/articles/PMC5481146?pdf=render |
| spellingShingle | Brendan J O'Hara Zachary K Barth Amelia C McKitterick Kimberley D Seed A highly specific phage defense system is a conserved feature of the Vibrio cholerae mobilome. PLoS Genetics |
| title | A highly specific phage defense system is a conserved feature of the Vibrio cholerae mobilome. |
| title_full | A highly specific phage defense system is a conserved feature of the Vibrio cholerae mobilome. |
| title_fullStr | A highly specific phage defense system is a conserved feature of the Vibrio cholerae mobilome. |
| title_full_unstemmed | A highly specific phage defense system is a conserved feature of the Vibrio cholerae mobilome. |
| title_short | A highly specific phage defense system is a conserved feature of the Vibrio cholerae mobilome. |
| title_sort | highly specific phage defense system is a conserved feature of the vibrio cholerae mobilome |
| url | http://europepmc.org/articles/PMC5481146?pdf=render |
| work_keys_str_mv | AT brendanjohara ahighlyspecificphagedefensesystemisaconservedfeatureofthevibriocholeraemobilome AT zacharykbarth ahighlyspecificphagedefensesystemisaconservedfeatureofthevibriocholeraemobilome AT ameliacmckitterick ahighlyspecificphagedefensesystemisaconservedfeatureofthevibriocholeraemobilome AT kimberleydseed ahighlyspecificphagedefensesystemisaconservedfeatureofthevibriocholeraemobilome AT brendanjohara highlyspecificphagedefensesystemisaconservedfeatureofthevibriocholeraemobilome AT zacharykbarth highlyspecificphagedefensesystemisaconservedfeatureofthevibriocholeraemobilome AT ameliacmckitterick highlyspecificphagedefensesystemisaconservedfeatureofthevibriocholeraemobilome AT kimberleydseed highlyspecificphagedefensesystemisaconservedfeatureofthevibriocholeraemobilome |