A Polymer Bio–Photoelectrolytic Platform for Electrical Signal Measurement and for Light Modulation of Ion Fluxes and Proliferation in a Neuroblastoma Cell Line
Light control of living systems is an emerging field in bioelectronics, in regenerative medicine and cell‐based therapy. Herein, the design of a semitransparent bio–photoelectrolytic platform for control of a neuroblastoma cell line via light pulses is laid out. The platform is based on conjugated p...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-05-01
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| Series: | Advanced NanoBiomed Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/anbr.202200127 |
| _version_ | 1797831045781913600 |
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| author | Manuela Ciocca Serena Marcozzi Paolo Mariani Valentina Lacconi Aldo Di Carlo Lucio Cinà Marcelo D. Rosato-Siri Alessandra Zanon Giada Cattelan Enrico Avancini Paolo Lugli Shashank Priya Antonella Camaioni Thomas M. Brown |
| author_facet | Manuela Ciocca Serena Marcozzi Paolo Mariani Valentina Lacconi Aldo Di Carlo Lucio Cinà Marcelo D. Rosato-Siri Alessandra Zanon Giada Cattelan Enrico Avancini Paolo Lugli Shashank Priya Antonella Camaioni Thomas M. Brown |
| author_sort | Manuela Ciocca |
| collection | DOAJ |
| description | Light control of living systems is an emerging field in bioelectronics, in regenerative medicine and cell‐based therapy. Herein, the design of a semitransparent bio–photoelectrolytic platform for control of a neuroblastoma cell line via light pulses is laid out. The platform is based on conjugated polymer films interfaced with a biological electrolyte solution confined in a compact chamber. Human SH‐SY5Y neuroblastoma cells are cultured for 3 days on the organic semiconductor and subjected to a pulsed light protocol. At the end of the culture time, proliferative activity of cells on the polymer film subjected to light pulses is reduced by 50% compared to the cultures kept in dark. An increase in intracellular Ca2+ level is observed, indicating a significant perturbation of the equilibrium potential of the cells. It is shown that the platform, in a sandwich‐type closed architecture with two transparent electrodes, can provide a tool for the initial recording of bioelectrical photovoltage signals (mV) that can complement analysis with more sophisticated electrophysiological tools. Obtained results can pave the way to new noninvasive photomanipulation techniques to stimulate/control living cells and their proliferation through both optical and electrical stimulation and probes, for application in the fields of biosensing and biomedicine. |
| first_indexed | 2024-04-09T13:45:46Z |
| format | Article |
| id | doaj.art-3bdf8600b8ac4968a3e4997da11afe08 |
| institution | Directory Open Access Journal |
| issn | 2699-9307 |
| language | English |
| last_indexed | 2024-04-09T13:45:46Z |
| publishDate | 2023-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced NanoBiomed Research |
| spelling | doaj.art-3bdf8600b8ac4968a3e4997da11afe082023-05-09T03:31:31ZengWiley-VCHAdvanced NanoBiomed Research2699-93072023-05-0135n/an/a10.1002/anbr.202200127A Polymer Bio–Photoelectrolytic Platform for Electrical Signal Measurement and for Light Modulation of Ion Fluxes and Proliferation in a Neuroblastoma Cell LineManuela Ciocca0Serena Marcozzi1Paolo Mariani2Valentina Lacconi3Aldo Di Carlo4Lucio Cinà5Marcelo D. Rosato-Siri6Alessandra Zanon7Giada Cattelan8Enrico Avancini9Paolo Lugli10Shashank Priya11Antonella Camaioni12Thomas M. Brown13Department of Electronic Engineering University of Rome Tor Vergata Via del Politecnico 1 00133 Rome ItalyDepartment of Biomedicine and Prevention University of Rome Tor Vergata Via Montpellier 1 00133 Rome ItalyDepartment of Electronic Engineering University of Rome Tor Vergata Via del Politecnico 1 00133 Rome ItalyDepartment of Biomedicine and Prevention University of Rome Tor Vergata Via Montpellier 1 00133 Rome ItalyIstituto di Struttura della Materia CNR-ISM via Fosso del Cavaliere 100 00133 Rome ItalyCicci Research srl. Via Giordania 227 58100 Grosseto ItalyInstitute for Biomedicine, Eurac Research Affiliated Institute of the University of Lübeck 39100 Bolzano ItalyInstitute for Biomedicine, Eurac Research Affiliated Institute of the University of Lübeck 39100 Bolzano ItalyInstitute for Biomedicine, Eurac Research Affiliated Institute of the University of Lübeck 39100 Bolzano ItalyFaculty of Science and Technology Free University of Bozen-Bolzano Piazza Università 1 39100 Bolzano ItalyFaculty of Science and Technology Free University of Bozen-Bolzano Piazza Università 1 39100 Bolzano ItalyDepartment of Materials Science and Engineering Pennsylvania State University University Park PA 16802 USADepartment of Biomedicine and Prevention University of Rome Tor Vergata Via Montpellier 1 00133 Rome ItalyDepartment of Electronic Engineering University of Rome Tor Vergata Via del Politecnico 1 00133 Rome ItalyLight control of living systems is an emerging field in bioelectronics, in regenerative medicine and cell‐based therapy. Herein, the design of a semitransparent bio–photoelectrolytic platform for control of a neuroblastoma cell line via light pulses is laid out. The platform is based on conjugated polymer films interfaced with a biological electrolyte solution confined in a compact chamber. Human SH‐SY5Y neuroblastoma cells are cultured for 3 days on the organic semiconductor and subjected to a pulsed light protocol. At the end of the culture time, proliferative activity of cells on the polymer film subjected to light pulses is reduced by 50% compared to the cultures kept in dark. An increase in intracellular Ca2+ level is observed, indicating a significant perturbation of the equilibrium potential of the cells. It is shown that the platform, in a sandwich‐type closed architecture with two transparent electrodes, can provide a tool for the initial recording of bioelectrical photovoltage signals (mV) that can complement analysis with more sophisticated electrophysiological tools. Obtained results can pave the way to new noninvasive photomanipulation techniques to stimulate/control living cells and their proliferation through both optical and electrical stimulation and probes, for application in the fields of biosensing and biomedicine.https://doi.org/10.1002/anbr.202200127bioelectronicscalcium signalingcell growth inhibitioncellular light stimulationneuroblastoma cell line SH-SY5Yorganic optoelectronics |
| spellingShingle | Manuela Ciocca Serena Marcozzi Paolo Mariani Valentina Lacconi Aldo Di Carlo Lucio Cinà Marcelo D. Rosato-Siri Alessandra Zanon Giada Cattelan Enrico Avancini Paolo Lugli Shashank Priya Antonella Camaioni Thomas M. Brown A Polymer Bio–Photoelectrolytic Platform for Electrical Signal Measurement and for Light Modulation of Ion Fluxes and Proliferation in a Neuroblastoma Cell Line Advanced NanoBiomed Research bioelectronics calcium signaling cell growth inhibition cellular light stimulation neuroblastoma cell line SH-SY5Y organic optoelectronics |
| title | A Polymer Bio–Photoelectrolytic Platform for Electrical Signal Measurement and for Light Modulation of Ion Fluxes and Proliferation in a Neuroblastoma Cell Line |
| title_full | A Polymer Bio–Photoelectrolytic Platform for Electrical Signal Measurement and for Light Modulation of Ion Fluxes and Proliferation in a Neuroblastoma Cell Line |
| title_fullStr | A Polymer Bio–Photoelectrolytic Platform for Electrical Signal Measurement and for Light Modulation of Ion Fluxes and Proliferation in a Neuroblastoma Cell Line |
| title_full_unstemmed | A Polymer Bio–Photoelectrolytic Platform for Electrical Signal Measurement and for Light Modulation of Ion Fluxes and Proliferation in a Neuroblastoma Cell Line |
| title_short | A Polymer Bio–Photoelectrolytic Platform for Electrical Signal Measurement and for Light Modulation of Ion Fluxes and Proliferation in a Neuroblastoma Cell Line |
| title_sort | polymer bio photoelectrolytic platform for electrical signal measurement and for light modulation of ion fluxes and proliferation in a neuroblastoma cell line |
| topic | bioelectronics calcium signaling cell growth inhibition cellular light stimulation neuroblastoma cell line SH-SY5Y organic optoelectronics |
| url | https://doi.org/10.1002/anbr.202200127 |
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