Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis
Abstract Rheumatoid arthritis (RA) is an autoimmune disorder that affects the joint synovium. Anserine is a functional dipeptide containing methylhistidine and β-alanine, and is present in the brain and skeletal muscle of birds and mammals. Glucosamine is an amino sugar used in the synthesis of glyc...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-03-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-020-00987-8 |
| _version_ | 1811339813107793920 |
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| author | Jiang Minghao Zhao Xiang Chen Ke Cheng Qingming Shi Kun Peng |
| author_facet | Jiang Minghao Zhao Xiang Chen Ke Cheng Qingming Shi Kun Peng |
| author_sort | Jiang Minghao Zhao |
| collection | DOAJ |
| description | Abstract Rheumatoid arthritis (RA) is an autoimmune disorder that affects the joint synovium. Anserine is a functional dipeptide containing methylhistidine and β-alanine, and is present in the brain and skeletal muscle of birds and mammals. Glucosamine is an amino sugar used in the synthesis of glycosylated proteins and lipids. We evaluated the effects of anserine and glucosamine on RA. Rats were assigned into the control group, RA group, anserine group (1 mg/kg), glucosamine group (200 mg/kg), or anserine plus glucosamine group (anserine, 1 mg/kg + glucosamine, 200 mg/kg). Treatment was continued for 45 consecutive days and was administered orally. The serum levels of catalase, glutathione peroxidase (Gpx), superoxide dismutase (SOD), reduced glutathione (GSH), lipid peroxidation, uric acid, nitric oxide, ceruloplasmin, zinc, copper, prostaglandin E2 (PGE2), matrix metalloproteinase (MMP)-3, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were assayed. The mRNA and protein levels of nuclear factor (NF)-κB and inducible nitric oxide synthase (iNOS) in synovial tissue were also determined. Anserine plus glucosamine significantly increased the catalase, SOD, Gpx, GSH, and zinc levels compared to the control, anserine, and glucosamine groups. Also, anserine plus glucosamine significantly reduced the PGE2, MMP-3, TNF-α, IL-1β, and IL-6 levels compared to the control, anserine, and glucosamine groups. Furthermore, anserine plus glucosamine significantly reduced the mRNA and protein levels of NF-κB and iNOS compared to the control, anserine, and glucosamine groups. Therefore, supplementation of anserine plus glucosamine shows therapeutic potential for RA. |
| first_indexed | 2024-04-13T18:32:06Z |
| format | Article |
| id | doaj.art-3be0b49a73824d0cbffadfa3f5402487 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-04-13T18:32:06Z |
| publishDate | 2020-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-3be0b49a73824d0cbffadfa3f54024872022-12-22T02:35:01ZengSpringerOpenAMB Express2191-08552020-03-011011710.1186/s13568-020-00987-8Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritisJiang Minghao Zhao0Xiang Chen1Ke Cheng2Qingming Shi3Kun Peng4Department of Orthopaedics, The Second Affiliated Hospital of Nanchang UniversityDepartment of Orthopaedics, The Second Affiliated Hospital of Nanchang UniversityDepartment of Orthopaedics, The Second Affiliated Hospital of Nanchang UniversityDepartment of Orthopaedics, The Second Affiliated Hospital of Nanchang UniversityDepartment of Orthopaedics, The Second Affiliated Hospital of Nanchang UniversityAbstract Rheumatoid arthritis (RA) is an autoimmune disorder that affects the joint synovium. Anserine is a functional dipeptide containing methylhistidine and β-alanine, and is present in the brain and skeletal muscle of birds and mammals. Glucosamine is an amino sugar used in the synthesis of glycosylated proteins and lipids. We evaluated the effects of anserine and glucosamine on RA. Rats were assigned into the control group, RA group, anserine group (1 mg/kg), glucosamine group (200 mg/kg), or anserine plus glucosamine group (anserine, 1 mg/kg + glucosamine, 200 mg/kg). Treatment was continued for 45 consecutive days and was administered orally. The serum levels of catalase, glutathione peroxidase (Gpx), superoxide dismutase (SOD), reduced glutathione (GSH), lipid peroxidation, uric acid, nitric oxide, ceruloplasmin, zinc, copper, prostaglandin E2 (PGE2), matrix metalloproteinase (MMP)-3, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were assayed. The mRNA and protein levels of nuclear factor (NF)-κB and inducible nitric oxide synthase (iNOS) in synovial tissue were also determined. Anserine plus glucosamine significantly increased the catalase, SOD, Gpx, GSH, and zinc levels compared to the control, anserine, and glucosamine groups. Also, anserine plus glucosamine significantly reduced the PGE2, MMP-3, TNF-α, IL-1β, and IL-6 levels compared to the control, anserine, and glucosamine groups. Furthermore, anserine plus glucosamine significantly reduced the mRNA and protein levels of NF-κB and iNOS compared to the control, anserine, and glucosamine groups. Therefore, supplementation of anserine plus glucosamine shows therapeutic potential for RA.http://link.springer.com/article/10.1186/s13568-020-00987-8Rheumatoid arthritisAnserineGlucosamineAntioxidantRats |
| spellingShingle | Jiang Minghao Zhao Xiang Chen Ke Cheng Qingming Shi Kun Peng Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis AMB Express Rheumatoid arthritis Anserine Glucosamine Antioxidant Rats |
| title | Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis |
| title_full | Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis |
| title_fullStr | Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis |
| title_full_unstemmed | Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis |
| title_short | Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis |
| title_sort | anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis |
| topic | Rheumatoid arthritis Anserine Glucosamine Antioxidant Rats |
| url | http://link.springer.com/article/10.1186/s13568-020-00987-8 |
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