FumDSB Can Reduce the Toxic Effects of Fumonisin B<sub>1</sub> by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs
Fumonisin B<sub>1</sub> (FB<sub>1</sub>) is the most common food-borne mycotoxin produced by the <i>Fusarium</i> species, posing a potential threat to human and animal health. Pigs are more sensitive to FB<sub>1</sub> ingested from feed compared to oth...
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MDPI AG
2021-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/12/874 |
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| author | Quancheng Liu Fuchang Li Libo Huang Wenjie Chen Zhongyuan Li Chunyang Wang |
| author_facet | Quancheng Liu Fuchang Li Libo Huang Wenjie Chen Zhongyuan Li Chunyang Wang |
| author_sort | Quancheng Liu |
| collection | DOAJ |
| description | Fumonisin B<sub>1</sub> (FB<sub>1</sub>) is the most common food-borne mycotoxin produced by the <i>Fusarium</i> species, posing a potential threat to human and animal health. Pigs are more sensitive to FB<sub>1</sub> ingested from feed compared to other farmed livestock. Enzymatic degradation is an ideal detoxification method that has attracted much attention. This study aimed to explore the functional characteristics of the carboxylesterase FumDSB in growing pigs from the perspective of brain–gut regulation. A total of 24 growing pigs were divided into three groups. The control group was fed a basal diet, the FB<sub>1</sub> group was supplemented with FB<sub>1</sub> at 5 mg/kg feed, and the FumDSB group received added FumDSB based on the diet of the FB<sub>1</sub> group. After 35 days of animal trials, samples from the hypothalamus and jejunum were analyzed through HE staining, qRT-PCR and immunohistochemistry. The results demonstrated that the ingestion of FB<sub>1</sub> can reduce the feed intake and weight gain of growing pigs, indicating that several appetite-related brain-gut peptides (including NPY, PYY, ghrelin and obestatin, etc.) play important roles in the anorexia response induced by FB<sub>1</sub>. After adding FumDSB as detoxifying enzymes, however, the anorexia effects of FB<sub>1</sub> were alleviated, and the expression and distribution of the corresponding brain-gut peptides exhibited a certain degree of regulation. In conclusion, the addition of FumDSB can reduce the anorexia effects of FB<sub>1</sub> by regulating several brain-gut peptides in both the hypothalamus and the jejunum of growing pigs. |
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| last_indexed | 2024-03-10T03:57:51Z |
| publishDate | 2021-12-01 |
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| series | Toxins |
| spelling | doaj.art-3be35a36df974c4f906f0f080d626c3d2023-11-23T10:50:46ZengMDPI AGToxins2072-66512021-12-01131287410.3390/toxins13120874FumDSB Can Reduce the Toxic Effects of Fumonisin B<sub>1</sub> by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing PigsQuancheng Liu0Fuchang Li1Libo Huang2Wenjie Chen3Zhongyuan Li4Chunyang Wang5Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaKey Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaFumonisin B<sub>1</sub> (FB<sub>1</sub>) is the most common food-borne mycotoxin produced by the <i>Fusarium</i> species, posing a potential threat to human and animal health. Pigs are more sensitive to FB<sub>1</sub> ingested from feed compared to other farmed livestock. Enzymatic degradation is an ideal detoxification method that has attracted much attention. This study aimed to explore the functional characteristics of the carboxylesterase FumDSB in growing pigs from the perspective of brain–gut regulation. A total of 24 growing pigs were divided into three groups. The control group was fed a basal diet, the FB<sub>1</sub> group was supplemented with FB<sub>1</sub> at 5 mg/kg feed, and the FumDSB group received added FumDSB based on the diet of the FB<sub>1</sub> group. After 35 days of animal trials, samples from the hypothalamus and jejunum were analyzed through HE staining, qRT-PCR and immunohistochemistry. The results demonstrated that the ingestion of FB<sub>1</sub> can reduce the feed intake and weight gain of growing pigs, indicating that several appetite-related brain-gut peptides (including NPY, PYY, ghrelin and obestatin, etc.) play important roles in the anorexia response induced by FB<sub>1</sub>. After adding FumDSB as detoxifying enzymes, however, the anorexia effects of FB<sub>1</sub> were alleviated, and the expression and distribution of the corresponding brain-gut peptides exhibited a certain degree of regulation. In conclusion, the addition of FumDSB can reduce the anorexia effects of FB<sub>1</sub> by regulating several brain-gut peptides in both the hypothalamus and the jejunum of growing pigs.https://www.mdpi.com/2072-6651/13/12/874fumonisin B<sub>1</sub>carboxylesterase FumDSBhypothalamusjejunumbrain-gut peptideghrelin |
| spellingShingle | Quancheng Liu Fuchang Li Libo Huang Wenjie Chen Zhongyuan Li Chunyang Wang FumDSB Can Reduce the Toxic Effects of Fumonisin B<sub>1</sub> by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs Toxins fumonisin B<sub>1</sub> carboxylesterase FumDSB hypothalamus jejunum brain-gut peptide ghrelin |
| title | FumDSB Can Reduce the Toxic Effects of Fumonisin B<sub>1</sub> by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs |
| title_full | FumDSB Can Reduce the Toxic Effects of Fumonisin B<sub>1</sub> by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs |
| title_fullStr | FumDSB Can Reduce the Toxic Effects of Fumonisin B<sub>1</sub> by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs |
| title_full_unstemmed | FumDSB Can Reduce the Toxic Effects of Fumonisin B<sub>1</sub> by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs |
| title_short | FumDSB Can Reduce the Toxic Effects of Fumonisin B<sub>1</sub> by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs |
| title_sort | fumdsb can reduce the toxic effects of fumonisin b sub 1 sub by regulating several brain gut peptides in both the hypothalamus and jejunum of growing pigs |
| topic | fumonisin B<sub>1</sub> carboxylesterase FumDSB hypothalamus jejunum brain-gut peptide ghrelin |
| url | https://www.mdpi.com/2072-6651/13/12/874 |
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