New Variants of Pseudomonas <i>aeruginosa</i> High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital
Recent multidrug resistance in <i>Pseudomonas aeruginosa</i> has favoured the adaptation and dissemination of worldwide high-risk strains. In June 2018, 15 <i>P. aeruginosa</i> strains isolated from patients and a contaminated multi-dose meropenem vial were characterized to a...
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MDPI AG
2022-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/8/1533 |
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| author | Pamela Aguilar-Rodea Elia L. Estrada-Javier Verónica Jiménez-Rojas Uriel Gomez-Ramirez Carolina G. Nolasco-Romero Gerardo E. Rodea Benjamín Antonio Rodríguez-Espino Sandra Mendoza-Elizalde Cesar Arellano Beatriz López-Marcelino Daniela de la Rosa Zamboni Ana Estela Gamiño-Arroyo Rosalia Mora-Suárez Margarita Torres García Isabel Franco Hernández Israel Parra-Ortega Guillermina Campos-Valdez Norma Velázquez-Guadarrama Irma Rosas-Pérez |
| author_facet | Pamela Aguilar-Rodea Elia L. Estrada-Javier Verónica Jiménez-Rojas Uriel Gomez-Ramirez Carolina G. Nolasco-Romero Gerardo E. Rodea Benjamín Antonio Rodríguez-Espino Sandra Mendoza-Elizalde Cesar Arellano Beatriz López-Marcelino Daniela de la Rosa Zamboni Ana Estela Gamiño-Arroyo Rosalia Mora-Suárez Margarita Torres García Isabel Franco Hernández Israel Parra-Ortega Guillermina Campos-Valdez Norma Velázquez-Guadarrama Irma Rosas-Pérez |
| author_sort | Pamela Aguilar-Rodea |
| collection | DOAJ |
| description | Recent multidrug resistance in <i>Pseudomonas aeruginosa</i> has favoured the adaptation and dissemination of worldwide high-risk strains. In June 2018, 15 <i>P. aeruginosa</i> strains isolated from patients and a contaminated multi-dose meropenem vial were characterized to assess their association to an outbreak in a Mexican paediatric hospital. The strains were characterized by antibiotic susceptibility profiling, virulence factors’ production, and biofilm formation. The clonal relationship among isolates was determined with pulse-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) sequencing. Repressor genes for the MexAB-OprM efflux pump were sequenced for haplotype identification. Of the strains, 60% were profiled as extensively drug-resistant (XDR), 33% as multidrug-resistant (MDR), and 6.6% were classified as sensitive (S). All strains presented intermediate resistance to colistin, and 80% were sensitive to aztreonam. Pyoverdine was the most produced virulence factor. The PFGE technique was performed for the identification of the outbreak, revealing eight strains with the same electrophoretic pattern. ST235 and ten new sequence types (STs) were identified, all closely related to ST233. ST3241 predominated in 26.66% of the strains. Twenty-five synonymous and seventeen nonsynonymous substitutions were identified in the regulatory genes of the MexAB-OprM efflux pump, and <i>nalC</i> was the most variable gene. Six different haplotypes were identified. Strains from the outbreak were metallo-β-lactamases and phylogenetically related to the high-risk clone ST233. |
| first_indexed | 2024-03-09T12:54:43Z |
| format | Article |
| id | doaj.art-3be733a319f94ea092f921033990f27b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T12:54:43Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3be733a319f94ea092f921033990f27b2023-11-30T22:01:31ZengMDPI AGMicroorganisms2076-26072022-07-01108153310.3390/microorganisms10081533New Variants of Pseudomonas <i>aeruginosa</i> High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric HospitalPamela Aguilar-Rodea0Elia L. Estrada-Javier1Verónica Jiménez-Rojas2Uriel Gomez-Ramirez3Carolina G. Nolasco-Romero4Gerardo E. Rodea5Benjamín Antonio Rodríguez-Espino6Sandra Mendoza-Elizalde7Cesar Arellano8Beatriz López-Marcelino9Daniela de la Rosa Zamboni10Ana Estela Gamiño-Arroyo11Rosalia Mora-Suárez12Margarita Torres García13Isabel Franco Hernández14Israel Parra-Ortega15Guillermina Campos-Valdez16Norma Velázquez-Guadarrama17Irma Rosas-Pérez18Área de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoLaboratorio de Investigación y Diagnóstico en Nefrología y Metabolismo Mineral Óseo, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoSubdirección de Atención Integral al Paciente, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoDepartamento de Epidemiología, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoDepartamento de Epidemiología, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoDepartamento de Epidemiología, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoDepartamento de Laboratorio Clínico, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoDepartamento de Laboratorio Clínico, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoÁrea de Genética Bacteriana, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Mexico City 06720, MexicoLaboratorio de Aerobiología, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, Mexico City 04510, MexicoRecent multidrug resistance in <i>Pseudomonas aeruginosa</i> has favoured the adaptation and dissemination of worldwide high-risk strains. In June 2018, 15 <i>P. aeruginosa</i> strains isolated from patients and a contaminated multi-dose meropenem vial were characterized to assess their association to an outbreak in a Mexican paediatric hospital. The strains were characterized by antibiotic susceptibility profiling, virulence factors’ production, and biofilm formation. The clonal relationship among isolates was determined with pulse-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) sequencing. Repressor genes for the MexAB-OprM efflux pump were sequenced for haplotype identification. Of the strains, 60% were profiled as extensively drug-resistant (XDR), 33% as multidrug-resistant (MDR), and 6.6% were classified as sensitive (S). All strains presented intermediate resistance to colistin, and 80% were sensitive to aztreonam. Pyoverdine was the most produced virulence factor. The PFGE technique was performed for the identification of the outbreak, revealing eight strains with the same electrophoretic pattern. ST235 and ten new sequence types (STs) were identified, all closely related to ST233. ST3241 predominated in 26.66% of the strains. Twenty-five synonymous and seventeen nonsynonymous substitutions were identified in the regulatory genes of the MexAB-OprM efflux pump, and <i>nalC</i> was the most variable gene. Six different haplotypes were identified. Strains from the outbreak were metallo-β-lactamases and phylogenetically related to the high-risk clone ST233.https://www.mdpi.com/2076-2607/10/8/1533<i>Pseudomonas aeruginosa</i>multidrug resistancehigh-risk clonesST233ST235outbreak |
| spellingShingle | Pamela Aguilar-Rodea Elia L. Estrada-Javier Verónica Jiménez-Rojas Uriel Gomez-Ramirez Carolina G. Nolasco-Romero Gerardo E. Rodea Benjamín Antonio Rodríguez-Espino Sandra Mendoza-Elizalde Cesar Arellano Beatriz López-Marcelino Daniela de la Rosa Zamboni Ana Estela Gamiño-Arroyo Rosalia Mora-Suárez Margarita Torres García Isabel Franco Hernández Israel Parra-Ortega Guillermina Campos-Valdez Norma Velázquez-Guadarrama Irma Rosas-Pérez New Variants of Pseudomonas <i>aeruginosa</i> High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital Microorganisms <i>Pseudomonas aeruginosa</i> multidrug resistance high-risk clones ST233 ST235 outbreak |
| title | New Variants of Pseudomonas <i>aeruginosa</i> High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital |
| title_full | New Variants of Pseudomonas <i>aeruginosa</i> High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital |
| title_fullStr | New Variants of Pseudomonas <i>aeruginosa</i> High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital |
| title_full_unstemmed | New Variants of Pseudomonas <i>aeruginosa</i> High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital |
| title_short | New Variants of Pseudomonas <i>aeruginosa</i> High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital |
| title_sort | new variants of pseudomonas i aeruginosa i high risk clone st233 associated with an outbreak in a mexican paediatric hospital |
| topic | <i>Pseudomonas aeruginosa</i> multidrug resistance high-risk clones ST233 ST235 outbreak |
| url | https://www.mdpi.com/2076-2607/10/8/1533 |
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