Laboratory detection and analysis of a suspected diarrhea outbreak caused by Clostridium perfringens
ObjectiveTo provide basic data for modification of laboratory testing for outbreaks caused by Clostridium perfringens （C. perfringens）， a diarrhea outbreak that may have been caused by polyclonal infection of C. perfringens was analyzed.MethodsAnal swab samples of 4 patients with diarrhea were colle...
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2023-07-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202307021 |
| _version_ | 1797563632141205504 |
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| author | ZHAO Fengling GAO Xiang ZHEN Bojun ZHANG Ping LUO Yuxin WANG Runping HUANG Zhen LI Ying ZOU Lin |
| author_facet | ZHAO Fengling GAO Xiang ZHEN Bojun ZHANG Ping LUO Yuxin WANG Runping HUANG Zhen LI Ying ZOU Lin |
| author_sort | ZHAO Fengling |
| collection | DOAJ |
| description | ObjectiveTo provide basic data for modification of laboratory testing for outbreaks caused by Clostridium perfringens （C. perfringens）， a diarrhea outbreak that may have been caused by polyclonal infection of C. perfringens was analyzed.MethodsAnal swab samples of 4 patients with diarrhea were collected. cpa and cpe genes and C. perfringens in original swab samples and enriched samples were detected via real time PCR. Toxin gene test and pulsed field gel electrophoresis （PFGE） were performed for some C. perfringens isolates.Resultscpa and cpe genes were positive in samples of 4 patients. One cpe+ isolate was obtained from 18 isolates of C. perfringens from patient 1， and the composition ratio was 5.56% （1/18）. One cpe+ isolate was obtained from 6 isolates of C. perfringens in patient 2， and the composition ratio was 16.7% （1/6）. No cpe+ isolate was obtained from patient 3， and C. perfringens was not isolated from patient 4. Two types （A type and C type） and 5 PFGE patterns were identified in 11 isolates of C. perfringens. Two cpe+ isolates from patient 1 and patient 2 had identical PFGE patterns.ConclusionThis outbreak might have been caused by C. perfringens. Considering the delays caused by the implementation of diagnostic standards， comprehensive use of laboratory detection methods can improve the response time of outbreak analysis. |
| first_indexed | 2024-03-10T18:46:15Z |
| format | Article |
| id | doaj.art-3bea093c648d4e7aa14020f4c4478922 |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-03-10T18:46:15Z |
| publishDate | 2023-07-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-3bea093c648d4e7aa14020f4c44789222023-11-20T05:28:59ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562023-07-013571109111310.13590/j.cjfh.2023.07.0211004-8456（2023）07-1109-05Laboratory detection and analysis of a suspected diarrhea outbreak caused by Clostridium perfringensZHAO Fengling0GAO Xiang1ZHEN Bojun2ZHANG Ping3LUO Yuxin4WANG Runping5HUANG Zhen6LI Ying7ZOU Lin8Tongzhou District Center for Disease Control and Prevention, Beijing 101100, ChinaTongzhou District Center for Disease Control and Prevention, Beijing 101100, ChinaTongzhou District Center for Disease Control and Prevention, Beijing 101100, ChinaTongzhou District Center for Disease Control and Prevention, Beijing 101100, ChinaTongzhou District Center for Disease Control and Prevention, Beijing 101100, ChinaTongzhou District Center for Disease Control and Prevention, Beijing 101100, ChinaTongzhou District Center for Disease Control and Prevention, Beijing 101100, ChinaShunyi District Center for Disease Control and Prevention, Beijing 101300, ChinaTongzhou District Center for Disease Control and Prevention, Beijing 101100, ChinaObjectiveTo provide basic data for modification of laboratory testing for outbreaks caused by Clostridium perfringens （C. perfringens）， a diarrhea outbreak that may have been caused by polyclonal infection of C. perfringens was analyzed.MethodsAnal swab samples of 4 patients with diarrhea were collected. cpa and cpe genes and C. perfringens in original swab samples and enriched samples were detected via real time PCR. Toxin gene test and pulsed field gel electrophoresis （PFGE） were performed for some C. perfringens isolates.Resultscpa and cpe genes were positive in samples of 4 patients. One cpe+ isolate was obtained from 18 isolates of C. perfringens from patient 1， and the composition ratio was 5.56% （1/18）. One cpe+ isolate was obtained from 6 isolates of C. perfringens in patient 2， and the composition ratio was 16.7% （1/6）. No cpe+ isolate was obtained from patient 3， and C. perfringens was not isolated from patient 4. Two types （A type and C type） and 5 PFGE patterns were identified in 11 isolates of C. perfringens. Two cpe+ isolates from patient 1 and patient 2 had identical PFGE patterns.ConclusionThis outbreak might have been caused by C. perfringens. Considering the delays caused by the implementation of diagnostic standards， comprehensive use of laboratory detection methods can improve the response time of outbreak analysis.http://www.zgspws.com/zgspwszz/article/abstract/202307021clostridium perfringensdiarrheaoutbreakpolyclonalpulsed field gel electrophoresistoxin gene |
| spellingShingle | ZHAO Fengling GAO Xiang ZHEN Bojun ZHANG Ping LUO Yuxin WANG Runping HUANG Zhen LI Ying ZOU Lin Laboratory detection and analysis of a suspected diarrhea outbreak caused by Clostridium perfringens Zhongguo shipin weisheng zazhi clostridium perfringens diarrhea outbreak polyclonal pulsed field gel electrophoresis toxin gene |
| title | Laboratory detection and analysis of a suspected diarrhea outbreak caused by Clostridium perfringens |
| title_full | Laboratory detection and analysis of a suspected diarrhea outbreak caused by Clostridium perfringens |
| title_fullStr | Laboratory detection and analysis of a suspected diarrhea outbreak caused by Clostridium perfringens |
| title_full_unstemmed | Laboratory detection and analysis of a suspected diarrhea outbreak caused by Clostridium perfringens |
| title_short | Laboratory detection and analysis of a suspected diarrhea outbreak caused by Clostridium perfringens |
| title_sort | laboratory detection and analysis of a suspected diarrhea outbreak caused by clostridium perfringens |
| topic | clostridium perfringens diarrhea outbreak polyclonal pulsed field gel electrophoresis toxin gene |
| url | http://www.zgspws.com/zgspwszz/article/abstract/202307021 |
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