Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the str...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/8/1487 |
| _version_ | 1797521854627315712 |
|---|---|
| author | Vladimira Horova Barbora Landova Jan Hodek Karel Chalupsky Petra Krafcikova Dominika Chalupska Vojtech Duchoslav Jan Weber Evzen Boura Martin Klima |
| author_facet | Vladimira Horova Barbora Landova Jan Hodek Karel Chalupsky Petra Krafcikova Dominika Chalupska Vojtech Duchoslav Jan Weber Evzen Boura Martin Klima |
| author_sort | Vladimira Horova |
| collection | DOAJ |
| description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2’-<i>O</i> MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2’-<i>O</i> RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2. |
| first_indexed | 2024-03-10T08:18:25Z |
| format | Article |
| id | doaj.art-3beeeef5e93940659d181dfbb4333ecf |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T08:18:25Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3beeeef5e93940659d181dfbb4333ecf2023-11-22T10:10:04ZengMDPI AGViruses1999-49152021-07-01138148710.3390/v13081487Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 SubunitVladimira Horova0Barbora Landova1Jan Hodek2Karel Chalupsky3Petra Krafcikova4Dominika Chalupska5Vojtech Duchoslav6Jan Weber7Evzen Boura8Martin Klima9Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech RepublicSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2’-<i>O</i> MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2’-<i>O</i> RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.https://www.mdpi.com/1999-4915/13/8/1487coronavirusSARS-CoV-2methyltransferasensp10nsp16nsp14 |
| spellingShingle | Vladimira Horova Barbora Landova Jan Hodek Karel Chalupsky Petra Krafcikova Dominika Chalupska Vojtech Duchoslav Jan Weber Evzen Boura Martin Klima Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit Viruses coronavirus SARS-CoV-2 methyltransferase nsp10 nsp16 nsp14 |
| title | Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit |
| title_full | Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit |
| title_fullStr | Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit |
| title_full_unstemmed | Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit |
| title_short | Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit |
| title_sort | localization of sars cov 2 capping enzymes revealed by an antibody against the nsp10 subunit |
| topic | coronavirus SARS-CoV-2 methyltransferase nsp10 nsp16 nsp14 |
| url | https://www.mdpi.com/1999-4915/13/8/1487 |
| work_keys_str_mv | AT vladimirahorova localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit AT barboralandova localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit AT janhodek localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit AT karelchalupsky localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit AT petrakrafcikova localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit AT dominikachalupska localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit AT vojtechduchoslav localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit AT janweber localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit AT evzenboura localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit AT martinklima localizationofsarscov2cappingenzymesrevealedbyanantibodyagainstthensp10subunit |