The mechanisms of Huangqi Guizhi Wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verification
AbstractContext Huangqi Guizhi Wuwu Decoction (HGWD) is effective in treating ischaemic stroke (IS). However, its mechanism of action is still unclear.Objective Network pharmacology integrated with in vivo experiments were used to clarify the underlying mechanisms of HGWD for treating IS.Materials a...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2023.2230477 |
| _version_ | 1797234823293566976 |
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| author | Weiguo Liao Minchun Wang Ying Wu Jinyan Du Yaxin Li Anyu Su Lanying Zhong Zi Xie Mingyu Gong Junhui Liang Pengcheng Wang Zai Liu Lisheng Wang |
| author_facet | Weiguo Liao Minchun Wang Ying Wu Jinyan Du Yaxin Li Anyu Su Lanying Zhong Zi Xie Mingyu Gong Junhui Liang Pengcheng Wang Zai Liu Lisheng Wang |
| author_sort | Weiguo Liao |
| collection | DOAJ |
| description | AbstractContext Huangqi Guizhi Wuwu Decoction (HGWD) is effective in treating ischaemic stroke (IS). However, its mechanism of action is still unclear.Objective Network pharmacology integrated with in vivo experiments were used to clarify the underlying mechanisms of HGWD for treating IS.Materials and methods TCMSP, GeneCards, OMIM and STRING were used to retrieve and construct visual protein interaction networks for the key targets. The AutoDock tool was used for molecular docking between key targets and active compounds. The neuroprotective effect of HGWD were verified in a middle cerebral artery occlusion (MCAO) model rat. The Sprague-Dawley (SD) rats were divided into sham, model, low-dose (5 g/kg, i.g.), high-dose (20 g/kg, i.g.), and nimodipine (20 mg/kg, i.g.) groups once daily for 7 days. The neurological scores, brain infarct volumes, lipid peroxidation, inflammatory cytokines, Nissl bodies, apoptotic neurons, and signalling pathways were all investigated and evaluated in vivo.Results Network pharmacology identified 117 HGWD targets related to IS and 36 candidate compounds. GO and KEGG analyses showed that HGWD anti-IS effects were mainly associated with PI3K-Akt and HIF-1 signalling pathways. HGWD effectively reduced the cerebral infarct volumes (19.19%), the number of apoptotic neurons (16.78%), and the release of inflammatory cytokines, etc. in MCAO rats. Furthermore, HGWD decreased the levels of HIF-1A, VEGFA, Bax, cleaved caspase-3, p-MAPK1, and p-c-Jun while increasing the expression of p-PI3K, p-AKT1, and Bcl-2.Discussion and conclusion This study initially elucidated the mechanism of HGWD anti-IS, which contributed to the further promotion and secondary development of HGWD in clinical practice. |
| first_indexed | 2024-03-09T00:08:58Z |
| format | Article |
| id | doaj.art-3bfad3e47a01423d965d1fc7a643f48f |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-24T16:38:11Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-3bfad3e47a01423d965d1fc7a643f48f2024-03-29T11:10:26ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162023-12-016111014102910.1080/13880209.2023.2230477The mechanisms of Huangqi Guizhi Wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verificationWeiguo Liao0Minchun Wang1Ying Wu2Jinyan Du3Yaxin Li4Anyu Su5Lanying Zhong6Zi Xie7Mingyu Gong8Junhui Liang9Pengcheng Wang10Zai Liu11Lisheng Wang12College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaPharmacy Department, Dongguan Hospital of Traditional Chinese Medicine, Dongguan, Guangdong, ChinaCollege of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, ChinaAbstractContext Huangqi Guizhi Wuwu Decoction (HGWD) is effective in treating ischaemic stroke (IS). However, its mechanism of action is still unclear.Objective Network pharmacology integrated with in vivo experiments were used to clarify the underlying mechanisms of HGWD for treating IS.Materials and methods TCMSP, GeneCards, OMIM and STRING were used to retrieve and construct visual protein interaction networks for the key targets. The AutoDock tool was used for molecular docking between key targets and active compounds. The neuroprotective effect of HGWD were verified in a middle cerebral artery occlusion (MCAO) model rat. The Sprague-Dawley (SD) rats were divided into sham, model, low-dose (5 g/kg, i.g.), high-dose (20 g/kg, i.g.), and nimodipine (20 mg/kg, i.g.) groups once daily for 7 days. The neurological scores, brain infarct volumes, lipid peroxidation, inflammatory cytokines, Nissl bodies, apoptotic neurons, and signalling pathways were all investigated and evaluated in vivo.Results Network pharmacology identified 117 HGWD targets related to IS and 36 candidate compounds. GO and KEGG analyses showed that HGWD anti-IS effects were mainly associated with PI3K-Akt and HIF-1 signalling pathways. HGWD effectively reduced the cerebral infarct volumes (19.19%), the number of apoptotic neurons (16.78%), and the release of inflammatory cytokines, etc. in MCAO rats. Furthermore, HGWD decreased the levels of HIF-1A, VEGFA, Bax, cleaved caspase-3, p-MAPK1, and p-c-Jun while increasing the expression of p-PI3K, p-AKT1, and Bcl-2.Discussion and conclusion This study initially elucidated the mechanism of HGWD anti-IS, which contributed to the further promotion and secondary development of HGWD in clinical practice.https://www.tandfonline.com/doi/10.1080/13880209.2023.2230477Chinese medicine formulamolecular dockingPI3K-Akt pathwayHIF-1 pathwaysinflammationapoptosis |
| spellingShingle | Weiguo Liao Minchun Wang Ying Wu Jinyan Du Yaxin Li Anyu Su Lanying Zhong Zi Xie Mingyu Gong Junhui Liang Pengcheng Wang Zai Liu Lisheng Wang The mechanisms of Huangqi Guizhi Wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verification Pharmaceutical Biology Chinese medicine formula molecular docking PI3K-Akt pathway HIF-1 pathways inflammation apoptosis |
| title | The mechanisms of Huangqi Guizhi Wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verification |
| title_full | The mechanisms of Huangqi Guizhi Wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verification |
| title_fullStr | The mechanisms of Huangqi Guizhi Wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verification |
| title_full_unstemmed | The mechanisms of Huangqi Guizhi Wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verification |
| title_short | The mechanisms of Huangqi Guizhi Wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verification |
| title_sort | mechanisms of huangqi guizhi wuwu decoction in treating ischaemic stroke based on network pharmacology and experiment verification |
| topic | Chinese medicine formula molecular docking PI3K-Akt pathway HIF-1 pathways inflammation apoptosis |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2023.2230477 |
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