Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads
The complex and lengthy protocol of current viral nucleic acid extraction processes limits their use outside laboratory settings. Here, we describe a rapid and reliable method for extracting nucleic acids from viral samples using a rotating blade and magnetic beads. The viral membrane can be instant...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/8/1995 |
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| author | Minju Bae Junsoo Park Hyeonah Seong Hansol Lee Wonsuk Choi Jiyun Noh Woojoo Kim Sehyun Shin |
| author_facet | Minju Bae Junsoo Park Hyeonah Seong Hansol Lee Wonsuk Choi Jiyun Noh Woojoo Kim Sehyun Shin |
| author_sort | Minju Bae |
| collection | DOAJ |
| description | The complex and lengthy protocol of current viral nucleic acid extraction processes limits their use outside laboratory settings. Here, we describe a rapid and reliable method for extracting nucleic acids from viral samples using a rotating blade and magnetic beads. The viral membrane can be instantly lysed using a high-speed rotating blade, and nucleic acids can be immediately isolated using a silica magnetic surface. The process was completed within 60 s by this method. Routine washing and eluting processes were subsequently conducted within 5 min. The results achieved by this method were comparable to those of a commercially available method. When the blade-based lysis and magnetic bead adsorption processes were performed separately, the RNA recovery rate was very low, and the Ct value was delayed compared to simultaneous lysis and RNA adsorption. Overall, this method not only dramatically shortens the conventional extraction time but also allows for its convenient use outside the laboratory, such as at remote field sites and for point-of-care testing. |
| first_indexed | 2024-03-09T09:58:10Z |
| format | Article |
| id | doaj.art-3bfce7c87aa6477181d3a320924dd87d |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T09:58:10Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-3bfce7c87aa6477181d3a320924dd87d2023-12-01T23:36:55ZengMDPI AGDiagnostics2075-44182022-08-01128199510.3390/diagnostics12081995Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic BeadsMinju Bae0Junsoo Park1Hyeonah Seong2Hansol Lee3Wonsuk Choi4Jiyun Noh5Woojoo Kim6Sehyun Shin7School of Mechanical Engineering, Korea University, Seoul 02841, KoreaDepartment of Micro-Nano Systems, Korea University, Seoul 02841, KoreaSchool of Mechanical Engineering, Korea University, Seoul 02841, KoreaAsia Pacific Influenza Institute, Korea University College of Medicine, Seoul 02841, KoreaDivision of Infectious Diseases, Department of Internal Medicine, Korea University College of Medicine, Seoul 02841, KoreaDivision of Infectious Diseases, Department of Internal Medicine, Korea University College of Medicine, Seoul 02841, KoreaAsia Pacific Influenza Institute, Korea University College of Medicine, Seoul 02841, KoreaSchool of Mechanical Engineering, Korea University, Seoul 02841, KoreaThe complex and lengthy protocol of current viral nucleic acid extraction processes limits their use outside laboratory settings. Here, we describe a rapid and reliable method for extracting nucleic acids from viral samples using a rotating blade and magnetic beads. The viral membrane can be instantly lysed using a high-speed rotating blade, and nucleic acids can be immediately isolated using a silica magnetic surface. The process was completed within 60 s by this method. Routine washing and eluting processes were subsequently conducted within 5 min. The results achieved by this method were comparable to those of a commercially available method. When the blade-based lysis and magnetic bead adsorption processes were performed separately, the RNA recovery rate was very low, and the Ct value was delayed compared to simultaneous lysis and RNA adsorption. Overall, this method not only dramatically shortens the conventional extraction time but also allows for its convenient use outside the laboratory, such as at remote field sites and for point-of-care testing.https://www.mdpi.com/2075-4418/12/8/1995virusnucleic acidextractionbladelysismagnetic beads |
| spellingShingle | Minju Bae Junsoo Park Hyeonah Seong Hansol Lee Wonsuk Choi Jiyun Noh Woojoo Kim Sehyun Shin Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads Diagnostics virus nucleic acid extraction blade lysis magnetic beads |
| title | Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads |
| title_full | Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads |
| title_fullStr | Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads |
| title_full_unstemmed | Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads |
| title_short | Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads |
| title_sort | rapid extraction of viral nucleic acids using rotating blade lysis and magnetic beads |
| topic | virus nucleic acid extraction blade lysis magnetic beads |
| url | https://www.mdpi.com/2075-4418/12/8/1995 |
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