Role of p16 testing in cervical cancer screening among HIV-infected women.
p16 immunohistochemistry is used to evaluate for HPV-associated cervical intraepithelial neoplasia. The diagnostic performance of p16 in HIV infection is unclear.Between June-December 2009, HIV-infected women underwent Papanicolaou (Pap) smear, human papillomavirus (HPV) testing, visual inspection w...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5638250?pdf=render |
| _version_ | 1819264788932329472 |
|---|---|
| author | Christine J McGrath Rochelle Garcia Trong T Trinh Barbra A Richardson Grace C John-Stewart Evans Nyongesa-Malava Nelly R Mugo Emily H Glynn Samah R Sakr Hugo De Vuyst Michael H Chung |
| author_facet | Christine J McGrath Rochelle Garcia Trong T Trinh Barbra A Richardson Grace C John-Stewart Evans Nyongesa-Malava Nelly R Mugo Emily H Glynn Samah R Sakr Hugo De Vuyst Michael H Chung |
| author_sort | Christine J McGrath |
| collection | DOAJ |
| description | p16 immunohistochemistry is used to evaluate for HPV-associated cervical intraepithelial neoplasia. The diagnostic performance of p16 in HIV infection is unclear.Between June-December 2009, HIV-infected women underwent Papanicolaou (Pap) smear, human papillomavirus (HPV) testing, visual inspection with acetic acid (VIA), and colposcopy-directed biopsy as the disease gold standard at a HIV clinic in Kenya. Pap smears were evaluated for p16 expression. Sensitivity, specificity, positive predictive value (PPV), and area under the receiver operating characteristic curve (AUC) of p16 to detect CIN2/3 on histology and the impact of immunosuppression and ART was assessed.Of 331 cervical samples with p16 expression, p16 sensitivity and specificity to detect CIN2/3 was 54.1% and 72.4% respectively, which was lower than Pap and HPV in sensitivity, but higher in specificity than Pap, HPV, and VIA. Combining tests and p16 reduced sensitivity and increased specificity of Pap from 90.5% to 48.7% and 51.4% to 81.7%; of VIA from 59.5% to 37.8% and 67.6% to 89.9%; and of HPV from 82.4% to 50.0% and 55.3% to 84.8%. Combination p16 increased the PPV of Pap from 34.9% to 43.4%; of HPV from 34.7% to 48.7%; and VIA from 34.9% to 51.9%. Adjunctive p16 did not change AUC (P>0.05). P16 performance was not altered by immunosuppression or ART use. Combining p16 with HPV and VIA reduced the variation in HPV and VIA performance associated with CD4 and ART.As an adjunctive test in HIV-infected women, p16 immunohistochemistry increased specificity and PPV of HPV and VIA for CIN2/3, and was not altered in performance by immunosuppression, ART, or age. |
| first_indexed | 2024-12-23T20:35:03Z |
| format | Article |
| id | doaj.art-3bffad5860f347a48297cf596f35f882 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T20:35:03Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3bffad5860f347a48297cf596f35f8822022-12-21T17:32:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011210e018559710.1371/journal.pone.0185597Role of p16 testing in cervical cancer screening among HIV-infected women.Christine J McGrathRochelle GarciaTrong T TrinhBarbra A RichardsonGrace C John-StewartEvans Nyongesa-MalavaNelly R MugoEmily H GlynnSamah R SakrHugo De VuystMichael H Chungp16 immunohistochemistry is used to evaluate for HPV-associated cervical intraepithelial neoplasia. The diagnostic performance of p16 in HIV infection is unclear.Between June-December 2009, HIV-infected women underwent Papanicolaou (Pap) smear, human papillomavirus (HPV) testing, visual inspection with acetic acid (VIA), and colposcopy-directed biopsy as the disease gold standard at a HIV clinic in Kenya. Pap smears were evaluated for p16 expression. Sensitivity, specificity, positive predictive value (PPV), and area under the receiver operating characteristic curve (AUC) of p16 to detect CIN2/3 on histology and the impact of immunosuppression and ART was assessed.Of 331 cervical samples with p16 expression, p16 sensitivity and specificity to detect CIN2/3 was 54.1% and 72.4% respectively, which was lower than Pap and HPV in sensitivity, but higher in specificity than Pap, HPV, and VIA. Combining tests and p16 reduced sensitivity and increased specificity of Pap from 90.5% to 48.7% and 51.4% to 81.7%; of VIA from 59.5% to 37.8% and 67.6% to 89.9%; and of HPV from 82.4% to 50.0% and 55.3% to 84.8%. Combination p16 increased the PPV of Pap from 34.9% to 43.4%; of HPV from 34.7% to 48.7%; and VIA from 34.9% to 51.9%. Adjunctive p16 did not change AUC (P>0.05). P16 performance was not altered by immunosuppression or ART use. Combining p16 with HPV and VIA reduced the variation in HPV and VIA performance associated with CD4 and ART.As an adjunctive test in HIV-infected women, p16 immunohistochemistry increased specificity and PPV of HPV and VIA for CIN2/3, and was not altered in performance by immunosuppression, ART, or age.http://europepmc.org/articles/PMC5638250?pdf=render |
| spellingShingle | Christine J McGrath Rochelle Garcia Trong T Trinh Barbra A Richardson Grace C John-Stewart Evans Nyongesa-Malava Nelly R Mugo Emily H Glynn Samah R Sakr Hugo De Vuyst Michael H Chung Role of p16 testing in cervical cancer screening among HIV-infected women. PLoS ONE |
| title | Role of p16 testing in cervical cancer screening among HIV-infected women. |
| title_full | Role of p16 testing in cervical cancer screening among HIV-infected women. |
| title_fullStr | Role of p16 testing in cervical cancer screening among HIV-infected women. |
| title_full_unstemmed | Role of p16 testing in cervical cancer screening among HIV-infected women. |
| title_short | Role of p16 testing in cervical cancer screening among HIV-infected women. |
| title_sort | role of p16 testing in cervical cancer screening among hiv infected women |
| url | http://europepmc.org/articles/PMC5638250?pdf=render |
| work_keys_str_mv | AT christinejmcgrath roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT rochellegarcia roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT trongttrinh roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT barbraarichardson roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT gracecjohnstewart roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT evansnyongesamalava roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT nellyrmugo roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT emilyhglynn roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT samahrsakr roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT hugodevuyst roleofp16testingincervicalcancerscreeningamonghivinfectedwomen AT michaelhchung roleofp16testingincervicalcancerscreeningamonghivinfectedwomen |