Effect of Ultrasonication Process on Microbial Content and Antioxidant Activities of Black Mulberry-Pineapple-Mango Functional Drink

Fruit juice may function as functional drink to improve human immune system. Fresh fruit-based functional drink contains high number of bioactive compounds, but it also may contain harmful microorganisms. The first objective of this work was to study the effect of ultrasonic amplitude and processing...

Full description

Bibliographic Details
Main Authors: Hui Qin Tan, Yanti Maslina Mohd Jusoh, Dayang Norulfairuz Abang Zaidel, Mazura Jusoh, Harisun Ya'akob, Daniel Joe Dailin
Format: Article
Language:English
Published: AIDIC Servizi S.r.l. 2021-02-01
Series:Chemical Engineering Transactions
Online Access:https://www.cetjournal.it/index.php/cet/article/view/11326
_version_ 1823928132728520704
author Hui Qin Tan
Yanti Maslina Mohd Jusoh
Dayang Norulfairuz Abang Zaidel
Mazura Jusoh
Harisun Ya'akob
Daniel Joe Dailin
author_facet Hui Qin Tan
Yanti Maslina Mohd Jusoh
Dayang Norulfairuz Abang Zaidel
Mazura Jusoh
Harisun Ya'akob
Daniel Joe Dailin
author_sort Hui Qin Tan
collection DOAJ
description Fruit juice may function as functional drink to improve human immune system. Fresh fruit-based functional drink contains high number of bioactive compounds, but it also may contain harmful microorganisms. The first objective of this work was to study the effect of ultrasonic amplitude and processing time on the microbial content and antioxidant activities of novel black mulberry-pineapple-mango functional drink. The second objective was to optimise the ultrasonic processing condition of the juice. Ultrasonic method was chosen in this study because it is a non-thermal processing technique which requires less energy consumption with shorter processing time. The study was conducted using Response Surface Methodology with ultrasonic amplitude (50 to 100 %) and processing time (4 to 12 min) as the investigated factors. The results show that ultrasonic treatment was able to reduce the microbial content yet maintained the antioxidant properties of the juice. The microbial content was significantly (p < 0.05) decreased, total phenolic content (TPC) and antioxidant activities (DPPH scavenging and FRAP) were significantly increased (p < 0.05) when high amplitude was applied. Increasing the processing time beyond 8.00 min was shown to cause negative impact towards total phenolic content and antioxidant activities of the drink. From optimisation, the best sonication condition for obtaining black mulberry-pineapple-mango functional drink with the lowest microbial content of 0.002 CFU/ml and highest TPC of 632.54 mg GAE/100 g, DPPH scavenging activity of 92.81 % and FRAP of 0.5127 mmol Fe2+/100 g is at amplitude of 100 % and processing time of 8.00 min. From the study, it can be concluded that ultrasonic processing is a potential alternative for fruit-based functional drink pasteurization.
first_indexed 2024-12-16T20:49:40Z
format Article
id doaj.art-3bffe3ea33cf483c9dd37b49d8061385
institution Directory Open Access Journal
issn 2283-9216
language English
last_indexed 2024-12-16T20:49:40Z
publishDate 2021-02-01
publisher AIDIC Servizi S.r.l.
record_format Article
series Chemical Engineering Transactions
spelling doaj.art-3bffe3ea33cf483c9dd37b49d80613852022-12-21T22:16:50ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162021-02-018310.3303/CET2183072Effect of Ultrasonication Process on Microbial Content and Antioxidant Activities of Black Mulberry-Pineapple-Mango Functional DrinkHui Qin TanYanti Maslina Mohd JusohDayang Norulfairuz Abang ZaidelMazura JusohHarisun Ya'akobDaniel Joe DailinFruit juice may function as functional drink to improve human immune system. Fresh fruit-based functional drink contains high number of bioactive compounds, but it also may contain harmful microorganisms. The first objective of this work was to study the effect of ultrasonic amplitude and processing time on the microbial content and antioxidant activities of novel black mulberry-pineapple-mango functional drink. The second objective was to optimise the ultrasonic processing condition of the juice. Ultrasonic method was chosen in this study because it is a non-thermal processing technique which requires less energy consumption with shorter processing time. The study was conducted using Response Surface Methodology with ultrasonic amplitude (50 to 100 %) and processing time (4 to 12 min) as the investigated factors. The results show that ultrasonic treatment was able to reduce the microbial content yet maintained the antioxidant properties of the juice. The microbial content was significantly (p < 0.05) decreased, total phenolic content (TPC) and antioxidant activities (DPPH scavenging and FRAP) were significantly increased (p < 0.05) when high amplitude was applied. Increasing the processing time beyond 8.00 min was shown to cause negative impact towards total phenolic content and antioxidant activities of the drink. From optimisation, the best sonication condition for obtaining black mulberry-pineapple-mango functional drink with the lowest microbial content of 0.002 CFU/ml and highest TPC of 632.54 mg GAE/100 g, DPPH scavenging activity of 92.81 % and FRAP of 0.5127 mmol Fe2+/100 g is at amplitude of 100 % and processing time of 8.00 min. From the study, it can be concluded that ultrasonic processing is a potential alternative for fruit-based functional drink pasteurization.https://www.cetjournal.it/index.php/cet/article/view/11326
spellingShingle Hui Qin Tan
Yanti Maslina Mohd Jusoh
Dayang Norulfairuz Abang Zaidel
Mazura Jusoh
Harisun Ya'akob
Daniel Joe Dailin
Effect of Ultrasonication Process on Microbial Content and Antioxidant Activities of Black Mulberry-Pineapple-Mango Functional Drink
Chemical Engineering Transactions
title Effect of Ultrasonication Process on Microbial Content and Antioxidant Activities of Black Mulberry-Pineapple-Mango Functional Drink
title_full Effect of Ultrasonication Process on Microbial Content and Antioxidant Activities of Black Mulberry-Pineapple-Mango Functional Drink
title_fullStr Effect of Ultrasonication Process on Microbial Content and Antioxidant Activities of Black Mulberry-Pineapple-Mango Functional Drink
title_full_unstemmed Effect of Ultrasonication Process on Microbial Content and Antioxidant Activities of Black Mulberry-Pineapple-Mango Functional Drink
title_short Effect of Ultrasonication Process on Microbial Content and Antioxidant Activities of Black Mulberry-Pineapple-Mango Functional Drink
title_sort effect of ultrasonication process on microbial content and antioxidant activities of black mulberry pineapple mango functional drink
url https://www.cetjournal.it/index.php/cet/article/view/11326
work_keys_str_mv AT huiqintan effectofultrasonicationprocessonmicrobialcontentandantioxidantactivitiesofblackmulberrypineapplemangofunctionaldrink
AT yantimaslinamohdjusoh effectofultrasonicationprocessonmicrobialcontentandantioxidantactivitiesofblackmulberrypineapplemangofunctionaldrink
AT dayangnorulfairuzabangzaidel effectofultrasonicationprocessonmicrobialcontentandantioxidantactivitiesofblackmulberrypineapplemangofunctionaldrink
AT mazurajusoh effectofultrasonicationprocessonmicrobialcontentandantioxidantactivitiesofblackmulberrypineapplemangofunctionaldrink
AT harisunyaakob effectofultrasonicationprocessonmicrobialcontentandantioxidantactivitiesofblackmulberrypineapplemangofunctionaldrink
AT danieljoedailin effectofultrasonicationprocessonmicrobialcontentandantioxidantactivitiesofblackmulberrypineapplemangofunctionaldrink