Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation.
In response to the signaling polyketide DIF-1 DimB directly activates transcription of the ecmB gene in pstB cells; a subset of the prestalk cells that are the precursors of the basal disc. We show that the promoter of pspA, a prespore-specific gene, also contains a DimB binding site. Mutation of th...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3253789?pdf=render |
| _version_ | 1811209133147291648 |
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| author | Beatriz Nuñez-Corcuera Joanna L Birch Yoko Yamada Jeffrey G Williams |
| author_facet | Beatriz Nuñez-Corcuera Joanna L Birch Yoko Yamada Jeffrey G Williams |
| author_sort | Beatriz Nuñez-Corcuera |
| collection | DOAJ |
| description | In response to the signaling polyketide DIF-1 DimB directly activates transcription of the ecmB gene in pstB cells; a subset of the prestalk cells that are the precursors of the basal disc. We show that the promoter of pspA, a prespore-specific gene, also contains a DimB binding site. Mutation of this site causes ectopic expression in the prestalk region and ChIP analysis shows that DIF-1 induces binding of DimB to the pspA promoter. DIF-1 represses pspA gene expression in a suspension cell assay but this repression is abrogated in a dimB null strain. These results suggest a coupled control mechanism, whereby the same DIF-DimB signaling pathway that directly activates ecmB gene expression directly represses pspA gene expression. |
| first_indexed | 2024-04-12T04:34:23Z |
| format | Article |
| id | doaj.art-3c0061c159494c76ad2ae7a288ee52ba |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:34:23Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3c0061c159494c76ad2ae7a288ee52ba2022-12-22T03:47:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0171e2989510.1371/journal.pone.0029895Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation.Beatriz Nuñez-CorcueraJoanna L BirchYoko YamadaJeffrey G WilliamsIn response to the signaling polyketide DIF-1 DimB directly activates transcription of the ecmB gene in pstB cells; a subset of the prestalk cells that are the precursors of the basal disc. We show that the promoter of pspA, a prespore-specific gene, also contains a DimB binding site. Mutation of this site causes ectopic expression in the prestalk region and ChIP analysis shows that DIF-1 induces binding of DimB to the pspA promoter. DIF-1 represses pspA gene expression in a suspension cell assay but this repression is abrogated in a dimB null strain. These results suggest a coupled control mechanism, whereby the same DIF-DimB signaling pathway that directly activates ecmB gene expression directly represses pspA gene expression.http://europepmc.org/articles/PMC3253789?pdf=render |
| spellingShingle | Beatriz Nuñez-Corcuera Joanna L Birch Yoko Yamada Jeffrey G Williams Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation. PLoS ONE |
| title | Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation. |
| title_full | Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation. |
| title_fullStr | Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation. |
| title_full_unstemmed | Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation. |
| title_short | Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation. |
| title_sort | transcriptional repression by a bzip protein regulates dictyostelium prespore differentiation |
| url | http://europepmc.org/articles/PMC3253789?pdf=render |
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