Species Diversity of Pin Nematodes (<i>Paratylenchus</i> spp.) from Potato Growing Regions of Southern Alberta, Canada
Pin nematodes (<i>Paratylenchus</i> spp.) are polyphagous parasitic species with a wide host range and geographical distribution; their diversity is unknown in the potato growing region of Alberta, Canada. The present study aims to provide morphological and molecular characterization of...
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MDPI AG
2021-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/2/188 |
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| author | Maria Munawar Dmytro P. Yevtushenko Juan E. Palomares-Rius Pablo Castillo |
| author_facet | Maria Munawar Dmytro P. Yevtushenko Juan E. Palomares-Rius Pablo Castillo |
| author_sort | Maria Munawar |
| collection | DOAJ |
| description | Pin nematodes (<i>Paratylenchus</i> spp.) are polyphagous parasitic species with a wide host range and geographical distribution; their diversity is unknown in the potato growing region of Alberta, Canada. The present study aims to provide morphological and molecular characterization of three pin nematode species, namely <i>P. neoprojectus</i>, <i>P. tateae</i>, and a new species, <i>Paratylenchus enigmaticus</i> sp. nov. All of them were recovered from the potato growing region of southern Alberta. The nematodes were isolated using the sieving and flotation-centrifugation method, and their morphology was assessed by light microscopy. Molecular characterization was performed using partial 18S, D2–D3 expansion domains of the 28S and ITS ribosomal genes. This study is the first report of molecular characterization of <i>P. tateae</i> and <i>P. neoprojectus</i>, being new records from southern Alberta, and two Spanish populations of <i>P. tateae</i> comprising the first report of this species in Europe. The phylogenetic analysis of the 18S, D2–D3 expansion domains of the 28S and ITS ribosomal DNA regions underscores the importance of using molecular data for accurate species identification and clarifies the status of <i>P. nanus</i> type B and <i>P. sheri</i>. Moreover, our findings will be useful to determine the impact of pin nematodes on potato production in future field research. |
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| language | English |
| last_indexed | 2024-03-09T04:16:18Z |
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| spelling | doaj.art-3c00b3c57229423cb5c3fbe559f5a8fe2023-12-03T13:53:38ZengMDPI AGPlants2223-77472021-01-0110218810.3390/plants10020188Species Diversity of Pin Nematodes (<i>Paratylenchus</i> spp.) from Potato Growing Regions of Southern Alberta, CanadaMaria Munawar0Dmytro P. Yevtushenko1Juan E. Palomares-Rius2Pablo Castillo3Department of Biological Sciences, University of Lethbridge, 4401 University Drive W, Lethbridge, AB T1K 3M4, CanadaDepartment of Biological Sciences, University of Lethbridge, 4401 University Drive W, Lethbridge, AB T1K 3M4, CanadaInstitute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, ceiA3, Avenida Menéndez Pidal s/n, 14004 Córdoba, SpainInstitute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, ceiA3, Avenida Menéndez Pidal s/n, 14004 Córdoba, SpainPin nematodes (<i>Paratylenchus</i> spp.) are polyphagous parasitic species with a wide host range and geographical distribution; their diversity is unknown in the potato growing region of Alberta, Canada. The present study aims to provide morphological and molecular characterization of three pin nematode species, namely <i>P. neoprojectus</i>, <i>P. tateae</i>, and a new species, <i>Paratylenchus enigmaticus</i> sp. nov. All of them were recovered from the potato growing region of southern Alberta. The nematodes were isolated using the sieving and flotation-centrifugation method, and their morphology was assessed by light microscopy. Molecular characterization was performed using partial 18S, D2–D3 expansion domains of the 28S and ITS ribosomal genes. This study is the first report of molecular characterization of <i>P. tateae</i> and <i>P. neoprojectus</i>, being new records from southern Alberta, and two Spanish populations of <i>P. tateae</i> comprising the first report of this species in Europe. The phylogenetic analysis of the 18S, D2–D3 expansion domains of the 28S and ITS ribosomal DNA regions underscores the importance of using molecular data for accurate species identification and clarifies the status of <i>P. nanus</i> type B and <i>P. sheri</i>. Moreover, our findings will be useful to determine the impact of pin nematodes on potato production in future field research.https://www.mdpi.com/2223-7747/10/2/188<i>Paratylenchus tateae</i><i>Paratylenchus neoprojectus</i>plant-parasitic nematodeintegrative taxonomymorphologyDNA sequencing |
| spellingShingle | Maria Munawar Dmytro P. Yevtushenko Juan E. Palomares-Rius Pablo Castillo Species Diversity of Pin Nematodes (<i>Paratylenchus</i> spp.) from Potato Growing Regions of Southern Alberta, Canada Plants <i>Paratylenchus tateae</i> <i>Paratylenchus neoprojectus</i> plant-parasitic nematode integrative taxonomy morphology DNA sequencing |
| title | Species Diversity of Pin Nematodes (<i>Paratylenchus</i> spp.) from Potato Growing Regions of Southern Alberta, Canada |
| title_full | Species Diversity of Pin Nematodes (<i>Paratylenchus</i> spp.) from Potato Growing Regions of Southern Alberta, Canada |
| title_fullStr | Species Diversity of Pin Nematodes (<i>Paratylenchus</i> spp.) from Potato Growing Regions of Southern Alberta, Canada |
| title_full_unstemmed | Species Diversity of Pin Nematodes (<i>Paratylenchus</i> spp.) from Potato Growing Regions of Southern Alberta, Canada |
| title_short | Species Diversity of Pin Nematodes (<i>Paratylenchus</i> spp.) from Potato Growing Regions of Southern Alberta, Canada |
| title_sort | species diversity of pin nematodes i paratylenchus i spp from potato growing regions of southern alberta canada |
| topic | <i>Paratylenchus tateae</i> <i>Paratylenchus neoprojectus</i> plant-parasitic nematode integrative taxonomy morphology DNA sequencing |
| url | https://www.mdpi.com/2223-7747/10/2/188 |
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