Preparation of apoE-free rat low density lipoprotein for catabolic studies.
The use of serum from rat fetuses instead of serum from adult rats for preparation of LDL by preparative ultracentrifugation leads to an LDL fraction containing apoB-100 and apoB-95 as the only protein moieties without need for any further purification. The yield of LDL is five times greater compare...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1987-12-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520385850 |
| _version_ | 1818862833767546880 |
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| author | B Schlag L Winkler D Plonné U Dürer R Dargel |
| author_facet | B Schlag L Winkler D Plonné U Dürer R Dargel |
| author_sort | B Schlag |
| collection | DOAJ |
| description | The use of serum from rat fetuses instead of serum from adult rats for preparation of LDL by preparative ultracentrifugation leads to an LDL fraction containing apoB-100 and apoB-95 as the only protein moieties without need for any further purification. The yield of LDL is five times greater compared to the use of adult rat serum. Lipid composition and particle size of LDL from fetal and adult rats are quite similar. The method described allows a simple way for preparation of sufficient amounts of apoE-free LDL for use in metabolic studies. |
| first_indexed | 2024-12-19T10:06:09Z |
| format | Article |
| id | doaj.art-3c01dc3e380c4e33b530267763eeed4a |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-19T10:06:09Z |
| publishDate | 1987-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-3c01dc3e380c4e33b530267763eeed4a2022-12-21T20:26:31ZengElsevierJournal of Lipid Research0022-22751987-12-01281215211524Preparation of apoE-free rat low density lipoprotein for catabolic studies.B Schlag0L Winkler1D Plonné2U Dürer3R Dargel4Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, GDR.The use of serum from rat fetuses instead of serum from adult rats for preparation of LDL by preparative ultracentrifugation leads to an LDL fraction containing apoB-100 and apoB-95 as the only protein moieties without need for any further purification. The yield of LDL is five times greater compared to the use of adult rat serum. Lipid composition and particle size of LDL from fetal and adult rats are quite similar. The method described allows a simple way for preparation of sufficient amounts of apoE-free LDL for use in metabolic studies.http://www.sciencedirect.com/science/article/pii/S0022227520385850 |
| spellingShingle | B Schlag L Winkler D Plonné U Dürer R Dargel Preparation of apoE-free rat low density lipoprotein for catabolic studies. Journal of Lipid Research |
| title | Preparation of apoE-free rat low density lipoprotein for catabolic studies. |
| title_full | Preparation of apoE-free rat low density lipoprotein for catabolic studies. |
| title_fullStr | Preparation of apoE-free rat low density lipoprotein for catabolic studies. |
| title_full_unstemmed | Preparation of apoE-free rat low density lipoprotein for catabolic studies. |
| title_short | Preparation of apoE-free rat low density lipoprotein for catabolic studies. |
| title_sort | preparation of apoe free rat low density lipoprotein for catabolic studies |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520385850 |
| work_keys_str_mv | AT bschlag preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies AT lwinkler preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies AT dplonne preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies AT udurer preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies AT rdargel preparationofapoefreeratlowdensitylipoproteinforcatabolicstudies |