In vivo Efficacy of HIFU (High Intensity Focused Ultrasound) on Mice with Ehrlich Ascites Carcinoma
Objective:Cavitation and coagulation necrosis due to high temperature caused by application of the high intensity focused ultrasound (HIFU) to a focal area occurs. This feature makes HIFU possible to use in the destruction of cancerous tissue. In this study, it was aimed to determine the effect of H...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2017-10-01
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| Series: | Bezmiâlem Science |
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http://bezmialemscience.org/archives/archive-detail/article-preview/n-vivo-efficacy-of-hfu-high-ntensity-focused-ultra/20240
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| _version_ | 1797912980317274112 |
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| author | Mustafa HASBAHÇECİ Tuğba KİRİŞ Gökhan ÇİPE Rasul SHARIFOV Zuhal GÜCİN Sadık KARA Öykü ONARAN Mahmut MÜSLÜMANOĞLU |
| author_facet | Mustafa HASBAHÇECİ Tuğba KİRİŞ Gökhan ÇİPE Rasul SHARIFOV Zuhal GÜCİN Sadık KARA Öykü ONARAN Mahmut MÜSLÜMANOĞLU |
| author_sort | Mustafa HASBAHÇECİ |
| collection | DOAJ |
| description | Objective:Cavitation and coagulation necrosis due to high temperature caused by application of the high intensity focused ultrasound (HIFU) to a focal area occurs. This feature makes HIFU possible to use in the destruction of cancerous tissue. In this study, it was aimed to determine the effect of HIFU on Balb/c mice with Ehrlich ascites tumor model to reach effective usage of HIFU with its clinical and experimental applications in various types of cancer.Methods:Ehrlich ascites tumor cells were obtained from the intraperitoneal region of a donor mouse. Fresh ehrlich ascites tumor cells were injected into 24 mice that were randomly distributed after tumor formation as a control (Group 1), sham treatment (Group 2) and HIFU treatment (Group 3). HIFU application was performed under ultrasound image until conversion of the hypoechoic tumor tissues to hyperechoic appearances. Tumor size and volumes were measured.Results:Tumor sizes and volumes in all groups showed an increase after the treatment. Pathologically all tumors were solid carcinoma with an expansive growth pattern. Necrosis percentages in Group 1, Group 2 and Group 3 were 70±9.6, 70±9.4 and 75.6±4.17, respectively. With regard to Volume 2, there were significant differences between Group 2 and both Group 1 and 3 (p=0.004 and p=0.0001, respectively).Discussion:High intensity focused ultrasound is an effective method in Ehrlich ascites tumor model via prevention of tumor growth and production of necrosis. To achieve a reduction in tumor volume, increasing the duration of HIFU treatment and/or repeatedly application of the treatment is thought to be effective. |
| first_indexed | 2024-04-10T12:04:24Z |
| format | Article |
| id | doaj.art-3c07ffad700146b893f57f985eed5341 |
| institution | Directory Open Access Journal |
| issn | 2148-2373 2148-2373 |
| language | English |
| last_indexed | 2024-04-10T12:04:24Z |
| publishDate | 2017-10-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Bezmiâlem Science |
| spelling | doaj.art-3c07ffad700146b893f57f985eed53412023-02-15T16:16:20ZengGalenos Publishing HouseBezmiâlem Science2148-23732148-23732017-10-015416817410.14235/bs.2017.141713049054In vivo Efficacy of HIFU (High Intensity Focused Ultrasound) on Mice with Ehrlich Ascites CarcinomaMustafa HASBAHÇECİ0Tuğba KİRİŞ1Gökhan ÇİPE2Rasul SHARIFOV3Zuhal GÜCİN4Sadık KARA5Öykü ONARAN6Mahmut MÜSLÜMANOĞLU7 Genel Cerrahi Anabilim Dalı, Bezmialem Vakıf Üniversitesi Tıp Fakültesi, İstanbul, Türkiye İstanbul Üniversitesi Fen Bilimleri Enstitüsü, İstanbul, Türkiye Medicana International Hastanesi, İstanbul, Türkiye Radyoloji Anabilim Dalı, Bezmialem Vakıf Üniversitesi Tıp Fakültesi, İstanbul, Türkiye Patoloji Anabilim Dalı, Bezmialem Vakıf Üniversitesi Tıp Fakültesi, İstanbul, Türkiye Emekli Öğretim Üyesi, İstanbul, Türkiye Tıp Fakültesi Öğrencisi, İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, İstanbul, Türkiye Genel Cerrahi Anabilim Dalı, İstanbul Üniversitesi Tıp Fakültesi, İstanbul, Türkiye Objective:Cavitation and coagulation necrosis due to high temperature caused by application of the high intensity focused ultrasound (HIFU) to a focal area occurs. This feature makes HIFU possible to use in the destruction of cancerous tissue. In this study, it was aimed to determine the effect of HIFU on Balb/c mice with Ehrlich ascites tumor model to reach effective usage of HIFU with its clinical and experimental applications in various types of cancer.Methods:Ehrlich ascites tumor cells were obtained from the intraperitoneal region of a donor mouse. Fresh ehrlich ascites tumor cells were injected into 24 mice that were randomly distributed after tumor formation as a control (Group 1), sham treatment (Group 2) and HIFU treatment (Group 3). HIFU application was performed under ultrasound image until conversion of the hypoechoic tumor tissues to hyperechoic appearances. Tumor size and volumes were measured.Results:Tumor sizes and volumes in all groups showed an increase after the treatment. Pathologically all tumors were solid carcinoma with an expansive growth pattern. Necrosis percentages in Group 1, Group 2 and Group 3 were 70±9.6, 70±9.4 and 75.6±4.17, respectively. With regard to Volume 2, there were significant differences between Group 2 and both Group 1 and 3 (p=0.004 and p=0.0001, respectively).Discussion:High intensity focused ultrasound is an effective method in Ehrlich ascites tumor model via prevention of tumor growth and production of necrosis. To achieve a reduction in tumor volume, increasing the duration of HIFU treatment and/or repeatedly application of the treatment is thought to be effective. http://bezmialemscience.org/archives/archive-detail/article-preview/n-vivo-efficacy-of-hfu-high-ntensity-focused-ultra/20240 High intensity focused ultrasoundEhrlich ascites carcinomabalb/c micecancer |
| spellingShingle | Mustafa HASBAHÇECİ Tuğba KİRİŞ Gökhan ÇİPE Rasul SHARIFOV Zuhal GÜCİN Sadık KARA Öykü ONARAN Mahmut MÜSLÜMANOĞLU In vivo Efficacy of HIFU (High Intensity Focused Ultrasound) on Mice with Ehrlich Ascites Carcinoma Bezmiâlem Science High intensity focused ultrasound Ehrlich ascites carcinoma balb/c mice cancer |
| title | In vivo Efficacy of HIFU (High Intensity Focused Ultrasound) on Mice with Ehrlich Ascites Carcinoma |
| title_full | In vivo Efficacy of HIFU (High Intensity Focused Ultrasound) on Mice with Ehrlich Ascites Carcinoma |
| title_fullStr | In vivo Efficacy of HIFU (High Intensity Focused Ultrasound) on Mice with Ehrlich Ascites Carcinoma |
| title_full_unstemmed | In vivo Efficacy of HIFU (High Intensity Focused Ultrasound) on Mice with Ehrlich Ascites Carcinoma |
| title_short | In vivo Efficacy of HIFU (High Intensity Focused Ultrasound) on Mice with Ehrlich Ascites Carcinoma |
| title_sort | in vivo efficacy of hifu high intensity focused ultrasound on mice with ehrlich ascites carcinoma |
| topic | High intensity focused ultrasound Ehrlich ascites carcinoma balb/c mice cancer |
| url |
http://bezmialemscience.org/archives/archive-detail/article-preview/n-vivo-efficacy-of-hfu-high-ntensity-focused-ultra/20240
|
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