Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice
Objective. To improve stool sample culture and isolation of anaerobic flora, including Clostridioides difficile in the routine microbiological laboratory practice at the children’s oncology hospital. Materials and Methods. A total of 517 stool samples collected from patients in children’s oncolo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy
2021-06-01
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| Series: | Клиническая микробиология и антимикробная химиотерапия |
| Subjects: | |
| Online Access: | https://cmac-journal.ru/publication/2021/2/cmac-2021-t23-n2-p212/cmac-2021-t23-n2-p212.pdf |
| _version_ | 1818894150861324288 |
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| author | Shvydkaya M.G. Zatevalov A.M. Mitrokhin S.D. Dzhandarova D.T. |
| author_facet | Shvydkaya M.G. Zatevalov A.M. Mitrokhin S.D. Dzhandarova D.T. |
| author_sort | Shvydkaya M.G. |
| collection | DOAJ |
| description | Objective.
To improve stool sample culture and isolation of anaerobic flora, including Clostridioides difficile in the routine microbiological laboratory practice at the children’s oncology hospital.
Materials and Methods.
A total of 517 stool samples collected from patients in children’s oncology hospital from 2013 to 2015 were studied. All samples were analyzed by ELISA for C. difficile toxins and by culture according to dedicated 5 schemes for isolation of anaerobic bacteria, including C. difficile. Statistical significance of differences in isolation rates between the studied groups (culture schemes) was assessed by Pearson test.
Results.
Culture in liver broth and covering with technical agar followed by culture on anaerobic agar yielded 100% isolation rate of toxigenic C. difficile strains. This culture scheme is also suitable for isolating concomitant anaerobic flora: non-toxigenic C. difficile strains, Clostridium perfringens, other Clostridia spp. and Bacteroides spp.
Conclusions.
Use of the liquid accumulation medium and covering with technical agar make it possible to isolate anaerobic flora from stool samples and increase an isolation rate of toxigenic C. difficile strains to 100% of ELISA-positive samples. |
| first_indexed | 2024-12-19T18:23:55Z |
| format | Article |
| id | doaj.art-3c080a2f0f4b495797bb0dabe05b45af |
| institution | Directory Open Access Journal |
| issn | 1684-4386 2686-9586 |
| language | Russian |
| last_indexed | 2024-12-19T18:23:55Z |
| publishDate | 2021-06-01 |
| publisher | Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy |
| record_format | Article |
| series | Клиническая микробиология и антимикробная химиотерапия |
| spelling | doaj.art-3c080a2f0f4b495797bb0dabe05b45af2022-12-21T20:10:54ZrusInterregional Association for Clinical Microbiology and Antimicrobial ChemotherapyКлиническая микробиология и антимикробная химиотерапия1684-43862686-95862021-06-0123221221610.36488/cmac.2021.2.212-216cmac-2021-t23-n2-p212Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practiceShvydkaya M.G.0Zatevalov A.M.1Mitrokhin S.D.2Dzhandarova D.T.3G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, RussiaG.N. Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, RussiaCity Clinical HospitalDiagnostic Clinical CenterObjective. To improve stool sample culture and isolation of anaerobic flora, including Clostridioides difficile in the routine microbiological laboratory practice at the children’s oncology hospital. Materials and Methods. A total of 517 stool samples collected from patients in children’s oncology hospital from 2013 to 2015 were studied. All samples were analyzed by ELISA for C. difficile toxins and by culture according to dedicated 5 schemes for isolation of anaerobic bacteria, including C. difficile. Statistical significance of differences in isolation rates between the studied groups (culture schemes) was assessed by Pearson test. Results. Culture in liver broth and covering with technical agar followed by culture on anaerobic agar yielded 100% isolation rate of toxigenic C. difficile strains. This culture scheme is also suitable for isolating concomitant anaerobic flora: non-toxigenic C. difficile strains, Clostridium perfringens, other Clostridia spp. and Bacteroides spp. Conclusions. Use of the liquid accumulation medium and covering with technical agar make it possible to isolate anaerobic flora from stool samples and increase an isolation rate of toxigenic C. difficile strains to 100% of ELISA-positive samples.https://cmac-journal.ru/publication/2021/2/cmac-2021-t23-n2-p212/cmac-2021-t23-n2-p212.pdfcultureliquid mediumstool samplesclostridium difficileanaerobes |
| spellingShingle | Shvydkaya M.G. Zatevalov A.M. Mitrokhin S.D. Dzhandarova D.T. Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice Клиническая микробиология и антимикробная химиотерапия culture liquid medium stool samples clostridium difficile anaerobes |
| title | Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice |
| title_full | Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice |
| title_fullStr | Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice |
| title_full_unstemmed | Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice |
| title_short | Comparison of culture and isolation methods for Clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice |
| title_sort | comparison of culture and isolation methods for clostridioides difficile and other anaerobes from stool samples in a routine microbiological laboratory practice |
| topic | culture liquid medium stool samples clostridium difficile anaerobes |
| url | https://cmac-journal.ru/publication/2021/2/cmac-2021-t23-n2-p212/cmac-2021-t23-n2-p212.pdf |
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