The RagA GTPase protects young egg chambers in Drosophila
Summary: The preservation of female fertility under unfavorable conditions is essential for animal reproduction. Inhibition of the target of rapamycin complex 1 (TORC1) is indispensable for Drosophila young egg chamber maintenance under nutrient starvation. Here, we show that knockdown of RagA resul...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723006423 |
| _version_ | 1797807036569747456 |
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| author | Ying Zhou Jianwen Guan Guoqiang Meng Weikang Fan Churui Ge Chunmei Niu Yang Cheng Yuanyuan Fu Yingying Lu Youheng Wei |
| author_facet | Ying Zhou Jianwen Guan Guoqiang Meng Weikang Fan Churui Ge Chunmei Niu Yang Cheng Yuanyuan Fu Yingying Lu Youheng Wei |
| author_sort | Ying Zhou |
| collection | DOAJ |
| description | Summary: The preservation of female fertility under unfavorable conditions is essential for animal reproduction. Inhibition of the target of rapamycin complex 1 (TORC1) is indispensable for Drosophila young egg chamber maintenance under nutrient starvation. Here, we show that knockdown of RagA results in young egg chamber death independent of TORC1 hyperactivity. RagA RNAi ovaries have autolysosomal acidification and degradation defects, which make the young egg chambers sensitive to autophagosome augmentation. Meanwhile, RagA RNAi ovaries have nuclear-localized Mitf, which promotes autophagic degradation and protects young egg chambers under stress. Interestingly, GDP-bound RagA rescues autolysosome defects, while GTP-bound RagA rescues Mitf nuclear localization in RagA RNAi young egg chambers. Moreover, Rag GTPase activity, rather than TORC1 activity, controls Mitf cellular localization in the Drosophila germ line. Our work suggests that RagA separately controls autolysosomal acidification and Mitf activity in the Drosophila young egg chambers. |
| first_indexed | 2024-03-13T06:16:29Z |
| format | Article |
| id | doaj.art-3c0927b96fea47c790fb0ab8b1905438 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-13T06:16:29Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-3c0927b96fea47c790fb0ab8b19054382023-06-11T04:14:28ZengElsevierCell Reports2211-12472023-06-01426112631The RagA GTPase protects young egg chambers in DrosophilaYing Zhou0Jianwen Guan1Guoqiang Meng2Weikang Fan3Churui Ge4Chunmei Niu5Yang Cheng6Yuanyuan Fu7Yingying Lu8Youheng Wei9Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaCollege of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; Institute of Reproduction and Metabolism, Yangzhou University, Yangzhou 225009, China; Corresponding authorSummary: The preservation of female fertility under unfavorable conditions is essential for animal reproduction. Inhibition of the target of rapamycin complex 1 (TORC1) is indispensable for Drosophila young egg chamber maintenance under nutrient starvation. Here, we show that knockdown of RagA results in young egg chamber death independent of TORC1 hyperactivity. RagA RNAi ovaries have autolysosomal acidification and degradation defects, which make the young egg chambers sensitive to autophagosome augmentation. Meanwhile, RagA RNAi ovaries have nuclear-localized Mitf, which promotes autophagic degradation and protects young egg chambers under stress. Interestingly, GDP-bound RagA rescues autolysosome defects, while GTP-bound RagA rescues Mitf nuclear localization in RagA RNAi young egg chambers. Moreover, Rag GTPase activity, rather than TORC1 activity, controls Mitf cellular localization in the Drosophila germ line. Our work suggests that RagA separately controls autolysosomal acidification and Mitf activity in the Drosophila young egg chambers.http://www.sciencedirect.com/science/article/pii/S2211124723006423CP: Developmental biology |
| spellingShingle | Ying Zhou Jianwen Guan Guoqiang Meng Weikang Fan Churui Ge Chunmei Niu Yang Cheng Yuanyuan Fu Yingying Lu Youheng Wei The RagA GTPase protects young egg chambers in Drosophila Cell Reports CP: Developmental biology |
| title | The RagA GTPase protects young egg chambers in Drosophila |
| title_full | The RagA GTPase protects young egg chambers in Drosophila |
| title_fullStr | The RagA GTPase protects young egg chambers in Drosophila |
| title_full_unstemmed | The RagA GTPase protects young egg chambers in Drosophila |
| title_short | The RagA GTPase protects young egg chambers in Drosophila |
| title_sort | raga gtpase protects young egg chambers in drosophila |
| topic | CP: Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723006423 |
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