Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors
Assessing levels of neuropeptide Y (NPY) in the human body has many medical uses. Accordingly, we report the quantitative detection of NPY biomarkers applying guided-mode resonance (GMR) biosensor methodology. The label-free sensor operates in the near-infrared spectral region exhibiting distinctive...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/1/126 |
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| author | Mohammad G. Abdallah Joseph A. Buchanan-Vega Kyu J. Lee Brett R. Wenner Jeffery W. Allen Monica S. Allen Susanne Gimlin Debra Wawro Weidanz Robert Magnusson |
| author_facet | Mohammad G. Abdallah Joseph A. Buchanan-Vega Kyu J. Lee Brett R. Wenner Jeffery W. Allen Monica S. Allen Susanne Gimlin Debra Wawro Weidanz Robert Magnusson |
| author_sort | Mohammad G. Abdallah |
| collection | DOAJ |
| description | Assessing levels of neuropeptide Y (NPY) in the human body has many medical uses. Accordingly, we report the quantitative detection of NPY biomarkers applying guided-mode resonance (GMR) biosensor methodology. The label-free sensor operates in the near-infrared spectral region exhibiting distinctive resonance signatures. The interaction of NPY with bioselective molecules on the sensor surface causes spectral shifts that directly identify the binding event without additional processing. In the experiments described here, NPY antibodies are attached to the sensor surface to impart specificity during operation. For the low concentrations of NPY of interest, we apply a sandwich NPY assay in which the sensor-linked anti-NPY molecule binds with NPY that subsequently binds with anti-NPY to close the sandwich. The sandwich assay achieves a detection limit of ~0.1 pM NPY. The photonic sensor methodology applied here enables expeditious high-throughput data acquisition with high sensitivity and specificity. The entire bioreaction is recorded as a function of time, in contrast to label-based methods with single-point detection. The convenient methodology and results reported are significant, as the NPY detection range of 0.1−10 pM demonstrated is useful in important medical circumstances. |
| first_indexed | 2024-04-12T19:45:10Z |
| format | Article |
| id | doaj.art-3c09357e5a4b466b8f9ade01b07150bf |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-12T19:45:10Z |
| publishDate | 2019-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-3c09357e5a4b466b8f9ade01b07150bf2022-12-22T03:18:59ZengMDPI AGSensors1424-82202019-12-0120112610.3390/s20010126s20010126Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance BiosensorsMohammad G. Abdallah0Joseph A. Buchanan-Vega1Kyu J. Lee2Brett R. Wenner3Jeffery W. Allen4Monica S. Allen5Susanne Gimlin6Debra Wawro Weidanz7Robert Magnusson8Department of Electrical Engineering, University of Texas Arlington, Arlington, TX 76019, USADepartment of Electrical Engineering, University of Texas Arlington, Arlington, TX 76019, USADepartment of Electrical Engineering, University of Texas Arlington, Arlington, TX 76019, USAAir Force Research Laboratory, Sensors Directorate, Wright-Patterson AFB, OH 45433, USAAir Force Research Laboratory, Munitions Directorate, Eglin AFB, FL 32542, USAAir Force Research Laboratory, Munitions Directorate, Eglin AFB, FL 32542, USAResonant Sensors Incorporated, Arlington, TX 76010, USAResonant Sensors Incorporated, Arlington, TX 76010, USADepartment of Electrical Engineering, University of Texas Arlington, Arlington, TX 76019, USAAssessing levels of neuropeptide Y (NPY) in the human body has many medical uses. Accordingly, we report the quantitative detection of NPY biomarkers applying guided-mode resonance (GMR) biosensor methodology. The label-free sensor operates in the near-infrared spectral region exhibiting distinctive resonance signatures. The interaction of NPY with bioselective molecules on the sensor surface causes spectral shifts that directly identify the binding event without additional processing. In the experiments described here, NPY antibodies are attached to the sensor surface to impart specificity during operation. For the low concentrations of NPY of interest, we apply a sandwich NPY assay in which the sensor-linked anti-NPY molecule binds with NPY that subsequently binds with anti-NPY to close the sandwich. The sandwich assay achieves a detection limit of ~0.1 pM NPY. The photonic sensor methodology applied here enables expeditious high-throughput data acquisition with high sensitivity and specificity. The entire bioreaction is recorded as a function of time, in contrast to label-based methods with single-point detection. The convenient methodology and results reported are significant, as the NPY detection range of 0.1−10 pM demonstrated is useful in important medical circumstances.https://www.mdpi.com/1424-8220/20/1/126biomarkersguided-mode resonance biosensorneuropeptide yoptical biosensorsandwich assay |
| spellingShingle | Mohammad G. Abdallah Joseph A. Buchanan-Vega Kyu J. Lee Brett R. Wenner Jeffery W. Allen Monica S. Allen Susanne Gimlin Debra Wawro Weidanz Robert Magnusson Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors Sensors biomarkers guided-mode resonance biosensor neuropeptide y optical biosensor sandwich assay |
| title | Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors |
| title_full | Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors |
| title_fullStr | Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors |
| title_full_unstemmed | Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors |
| title_short | Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors |
| title_sort | quantification of neuropeptide y with picomolar sensitivity enabled by guided mode resonance biosensors |
| topic | biomarkers guided-mode resonance biosensor neuropeptide y optical biosensor sandwich assay |
| url | https://www.mdpi.com/1424-8220/20/1/126 |
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