Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18)
Microbial-assisted phytoremediation promotes the ecological restoration of high and steep rocky slopes. To determine the structure and function of microbial communities in the soil in response to changes in soil nutrient content, the bacterial communities of rhizospheric soil from three types of pla...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.926037/full |
| _version_ | 1828187848824586240 |
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| author | Chao Liu Jiayao Zhuang Jie Wang Guohua Fan Ming Feng Shutong Zhang |
| author_facet | Chao Liu Jiayao Zhuang Jie Wang Guohua Fan Ming Feng Shutong Zhang |
| author_sort | Chao Liu |
| collection | DOAJ |
| description | Microbial-assisted phytoremediation promotes the ecological restoration of high and steep rocky slopes. To determine the structure and function of microbial communities in the soil in response to changes in soil nutrient content, the bacterial communities of rhizospheric soil from three types of plants, i.e., Robinia pseudoacacia, Pinus massoniana, and Cynodon dactylon, were analyzed using Illumina sequencing technology. High-quality sequences were clustered at the 97% similarity level. The dominant genera were found to be RB41, Gemmatimonas, Sphingomonas, Bradyrhizobium, and Ellin6067. The Tukey HSD (honestly significant difference) test results showed that the abundance of RB41 and Gemmatimonas were significantly different among three types of plants (p < 0.01). The relative abundances of RB41 (13.32%) and Gemmatimonas (3.36%) in rhizospheric soil samples from R. pseudoacacia were significantly higher than that from P. massoniana (0.16 and 0.35%) and C. dactylon (0.40 and 0.82%), respectively. The soil chemical properties analyses suggested that significant differences in rhizospheric soil nutrient content among the three plant types. Especially the available phosphorus, the content of it in the rhizospheric soil of R. pseudoacacia was about 280% (P. massoniana) and 58% (C. dactylon) higher than that of the other two plants, respectively. The soil bacterial communities were further studied using the correlation analysis and the Tax4Fun analysis. A significant and positive correlation was observed between Gemmatimonas and soil nutrient components. Except total nitrogen, the positive correlation between Gemmatimonas and other soil nutrient components was above 0.9. The outcomes of these analyses suggested that Gemmatimonas could be the indicator genus in response to changes in the soil nutrient content. Besides, the genes involved in metabolism were the major contributor to soil nutrients. This study showed that soil nutrients affect the soil bacterial community structure and function. In addition, pot experiments showed that Microbacterium invictum X-18 isolated from the rhizospheric soil of R. pseudoacacia significantly improved soil nutrient content and increased R. pseudoacacia growth. A significant increase in the numbers of nodules of R. pseudoacacia and an increase of 28% in plant height, accompanied by an increase of 94% in available phosphorus was measured in the M. invictum X-18 treatment than the control treatment. |
| first_indexed | 2024-04-12T07:41:59Z |
| format | Article |
| id | doaj.art-3c09ab77b61345ea8c15f9073271f397 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T07:41:59Z |
| publishDate | 2022-08-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-3c09ab77b61345ea8c15f9073271f3972022-12-22T03:41:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-08-011310.3389/fmicb.2022.926037926037Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18)Chao Liu0Jiayao Zhuang1Jie Wang2Guohua Fan3Ming Feng4Shutong Zhang5Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, ChinaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, ChinaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, ChinaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, ChinaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing, ChinaChina National Chemical Construction Investment Group Co., Ltd, Beijing, ChinaMicrobial-assisted phytoremediation promotes the ecological restoration of high and steep rocky slopes. To determine the structure and function of microbial communities in the soil in response to changes in soil nutrient content, the bacterial communities of rhizospheric soil from three types of plants, i.e., Robinia pseudoacacia, Pinus massoniana, and Cynodon dactylon, were analyzed using Illumina sequencing technology. High-quality sequences were clustered at the 97% similarity level. The dominant genera were found to be RB41, Gemmatimonas, Sphingomonas, Bradyrhizobium, and Ellin6067. The Tukey HSD (honestly significant difference) test results showed that the abundance of RB41 and Gemmatimonas were significantly different among three types of plants (p < 0.01). The relative abundances of RB41 (13.32%) and Gemmatimonas (3.36%) in rhizospheric soil samples from R. pseudoacacia were significantly higher than that from P. massoniana (0.16 and 0.35%) and C. dactylon (0.40 and 0.82%), respectively. The soil chemical properties analyses suggested that significant differences in rhizospheric soil nutrient content among the three plant types. Especially the available phosphorus, the content of it in the rhizospheric soil of R. pseudoacacia was about 280% (P. massoniana) and 58% (C. dactylon) higher than that of the other two plants, respectively. The soil bacterial communities were further studied using the correlation analysis and the Tax4Fun analysis. A significant and positive correlation was observed between Gemmatimonas and soil nutrient components. Except total nitrogen, the positive correlation between Gemmatimonas and other soil nutrient components was above 0.9. The outcomes of these analyses suggested that Gemmatimonas could be the indicator genus in response to changes in the soil nutrient content. Besides, the genes involved in metabolism were the major contributor to soil nutrients. This study showed that soil nutrients affect the soil bacterial community structure and function. In addition, pot experiments showed that Microbacterium invictum X-18 isolated from the rhizospheric soil of R. pseudoacacia significantly improved soil nutrient content and increased R. pseudoacacia growth. A significant increase in the numbers of nodules of R. pseudoacacia and an increase of 28% in plant height, accompanied by an increase of 94% in available phosphorus was measured in the M. invictum X-18 treatment than the control treatment.https://www.frontiersin.org/articles/10.3389/fmicb.2022.926037/fullphytoremediationbacterial communitiessoil nutrientsequencing technologybioinoculantplant-growth promotion |
| spellingShingle | Chao Liu Jiayao Zhuang Jie Wang Guohua Fan Ming Feng Shutong Zhang Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18) Frontiers in Microbiology phytoremediation bacterial communities soil nutrient sequencing technology bioinoculant plant-growth promotion |
| title | Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18) |
| title_full | Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18) |
| title_fullStr | Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18) |
| title_full_unstemmed | Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18) |
| title_short | Soil bacterial communities of three types of plants from ecological restoration areas and plant-growth promotional benefits of Microbacterium invictum (strain X-18) |
| title_sort | soil bacterial communities of three types of plants from ecological restoration areas and plant growth promotional benefits of microbacterium invictum strain x 18 |
| topic | phytoremediation bacterial communities soil nutrient sequencing technology bioinoculant plant-growth promotion |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.926037/full |
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