Evaluation of the Method of Binding Tamoxifen to DNA Experimentally and Computationally
Background and Aim: Tamoxifen is a group of drugs of selective estrogen receptor modulators, and is one of the drugs effective in the prevention and treatment of some cancers (such as breast cancer). In this study, the interaction of tamoxifen with DNA is investigated experimentally. Also, the elect...
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| Format: | Article |
| Language: | fas |
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Arak Medical University
2021-09-01
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| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://jams.arakmu.ac.ir/article-1-6419-en.html |
| _version_ | 1811334369254572032 |
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| author | Fatemeh Sharafi Bajgan Reza Safari Maryam Nejat Dehkordi |
| author_facet | Fatemeh Sharafi Bajgan Reza Safari Maryam Nejat Dehkordi |
| author_sort | Fatemeh Sharafi Bajgan |
| collection | DOAJ |
| description | Background and Aim: Tamoxifen is a group of drugs of selective estrogen receptor modulators, and is one of the drugs effective in the prevention and treatment of some cancers (such as breast cancer). In this study, the interaction of tamoxifen with DNA is investigated experimentally. Also, the electronic structure (at atomic scale) of the molecular system of tamoxifen was theoretically investigated, using atom in molecule (AIM) theory.
Methods & Materials: First, in the experimental section of this study, the interaction of Tamoxifen with DNA were investigated by UV-ViS technique and hydrodynamic method (Viscometry). In addition, the analysis of the experimental results shows the obvious effect of concentration on the mechanism of how the tamoxifen molecule binds to DNA. Then, in the theoretical part of this research, using computational biophysical chemistry methods, some properties of tamoxifen molecular system, such as electronic Density of States (DOS), boundary orbital’s energy (HOMO/LUMO), Electrostatic Potential Energy (EPS) and electronic contour maps of the electron density and its Laplacian, will be calculated.
Ethical Considerations: This article is a meta-analysis with animal sample.
Results: Result of the UV-ViS spectroscopy technique and viscometry indicated hyperchromism and hypochromism effect. In addition, the result were depend on the concentration of the drug and affected the kind of binding of Tamoxifen to DNA. the analysis of computational studies on the drug tamoxifen suggests that the mechanism of the local charge/energy distribution in the molecular system of tamoxifen plays an important role in how this drug binds to DNA.
Conclusion: Based on the experimental results of UV-ViS technique and viscometry, as well as the electronic/vibrational properties of the tamoxifen molecular system, it was defined that the Tamoxifen interacts significantly with all the binding sites of DNA. |
| first_indexed | 2024-04-13T17:07:49Z |
| format | Article |
| id | doaj.art-3c0a482fcb254c86b41283a48d77766f |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-04-13T17:07:49Z |
| publishDate | 2021-09-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-3c0a482fcb254c86b41283a48d77766f2022-12-22T02:38:24ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2021-09-01244538553Evaluation of the Method of Binding Tamoxifen to DNA Experimentally and ComputationallyFatemeh Sharafi Bajgan0Reza Safari1Maryam Nejat Dehkordi2 Department of Chemistry, Faculty of Science, University of Qom, Qom, Iran. Department of Chemistry, Faculty of Science, University of Qom, Qom, Iran. Department of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. Background and Aim: Tamoxifen is a group of drugs of selective estrogen receptor modulators, and is one of the drugs effective in the prevention and treatment of some cancers (such as breast cancer). In this study, the interaction of tamoxifen with DNA is investigated experimentally. Also, the electronic structure (at atomic scale) of the molecular system of tamoxifen was theoretically investigated, using atom in molecule (AIM) theory. Methods & Materials: First, in the experimental section of this study, the interaction of Tamoxifen with DNA were investigated by UV-ViS technique and hydrodynamic method (Viscometry). In addition, the analysis of the experimental results shows the obvious effect of concentration on the mechanism of how the tamoxifen molecule binds to DNA. Then, in the theoretical part of this research, using computational biophysical chemistry methods, some properties of tamoxifen molecular system, such as electronic Density of States (DOS), boundary orbital’s energy (HOMO/LUMO), Electrostatic Potential Energy (EPS) and electronic contour maps of the electron density and its Laplacian, will be calculated. Ethical Considerations: This article is a meta-analysis with animal sample. Results: Result of the UV-ViS spectroscopy technique and viscometry indicated hyperchromism and hypochromism effect. In addition, the result were depend on the concentration of the drug and affected the kind of binding of Tamoxifen to DNA. the analysis of computational studies on the drug tamoxifen suggests that the mechanism of the local charge/energy distribution in the molecular system of tamoxifen plays an important role in how this drug binds to DNA. Conclusion: Based on the experimental results of UV-ViS technique and viscometry, as well as the electronic/vibrational properties of the tamoxifen molecular system, it was defined that the Tamoxifen interacts significantly with all the binding sites of DNA.http://jams.arakmu.ac.ir/article-1-6419-en.htmltamoxifendnabindingspectroscopycomputational chemistry / biology |
| spellingShingle | Fatemeh Sharafi Bajgan Reza Safari Maryam Nejat Dehkordi Evaluation of the Method of Binding Tamoxifen to DNA Experimentally and Computationally Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk tamoxifen dna binding spectroscopy computational chemistry / biology |
| title | Evaluation of the Method of Binding Tamoxifen to DNA Experimentally and Computationally |
| title_full | Evaluation of the Method of Binding Tamoxifen to DNA Experimentally and Computationally |
| title_fullStr | Evaluation of the Method of Binding Tamoxifen to DNA Experimentally and Computationally |
| title_full_unstemmed | Evaluation of the Method of Binding Tamoxifen to DNA Experimentally and Computationally |
| title_short | Evaluation of the Method of Binding Tamoxifen to DNA Experimentally and Computationally |
| title_sort | evaluation of the method of binding tamoxifen to dna experimentally and computationally |
| topic | tamoxifen dna binding spectroscopy computational chemistry / biology |
| url | http://jams.arakmu.ac.ir/article-1-6419-en.html |
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