Renal macrophage infiltration is associated with a poor outcome in IgA nephropathy
OBJECTIVES: The objectives of our study were as follows: 1) to analyze the prognostic value of macrophage infiltration in primary IgA nephropathy (IgAN) and 2) to study the relationship between macrophages and other factors associated with the development of renal fibrosis, including mast cells, TGF...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier España
2012-07-01
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| Series: | Clinics |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012000700001 |
| _version_ | 1811330708309803008 |
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| author | Gyl Eanes Barros Silva Roberto Silva Costa Roberto Cuan Ravinal Leandra Naira Zambelli Ramalho Marlene Antonia dos Reis Miguel Moyses-Neto Elen Almeida Romao Terezila Machado Coimbra Márcio Dantas |
| author_facet | Gyl Eanes Barros Silva Roberto Silva Costa Roberto Cuan Ravinal Leandra Naira Zambelli Ramalho Marlene Antonia dos Reis Miguel Moyses-Neto Elen Almeida Romao Terezila Machado Coimbra Márcio Dantas |
| author_sort | Gyl Eanes Barros Silva |
| collection | DOAJ |
| description | OBJECTIVES: The objectives of our study were as follows: 1) to analyze the prognostic value of macrophage infiltration in primary IgA nephropathy (IgAN) and 2) to study the relationship between macrophages and other factors associated with the development of renal fibrosis, including mast cells, TGF-β1, α-SMA and NF-kB. METHODS: We analyzed 62 patients who had been diagnosed with IgAN between 1987 and 2003. Immunohistochemical staining was performed with monoclonal antibodies against CD68 and mast cell tryptase and polyclonal antibodies against TGF-β1, α-SMA and NF-kB p65. We also used Southwestern histochemistry for the in situ detection of activated NF-kB. RESULTS: The infiltration of macrophages into the tubulointerstitial compartment correlated with unfavorable clinical and histological parameters, and a worse clinical course of IgAN was significantly associated with the number of tubulointerstitial macrophages. Kaplan-Meier curves demonstrated that increased macrophage infiltration was associated with decreased renal survival. Moreover, the presence of macrophages was associated with mast cells, tubulointerstitial α-SMA expression and NF-kB activation (IH and Southwestern histochemistry). In the multivariate analysis, the two parameters that correlated with macrophage infiltration, proteinuria and tubulointerstitial injury, were independently associated with an unfavorable clinical course. CONCLUSION: An increased number of macrophages in the tubulointerstitial area may serve as a predictive factor for poor prognosis in patients with IgAN, and these cells were also associated with the expression of pro-fibrotic factors. |
| first_indexed | 2024-04-13T16:06:36Z |
| format | Article |
| id | doaj.art-3c0d7f553e3b44c49601fd39fb0ae883 |
| institution | Directory Open Access Journal |
| issn | 1807-5932 1980-5322 |
| language | English |
| last_indexed | 2024-04-13T16:06:36Z |
| publishDate | 2012-07-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-3c0d7f553e3b44c49601fd39fb0ae8832022-12-22T02:40:22ZengElsevier EspañaClinics1807-59321980-53222012-07-0167769770310.6061/clinics/2012(07)01Renal macrophage infiltration is associated with a poor outcome in IgA nephropathyGyl Eanes Barros SilvaRoberto Silva CostaRoberto Cuan RavinalLeandra Naira Zambelli RamalhoMarlene Antonia dos ReisMiguel Moyses-NetoElen Almeida RomaoTerezila Machado CoimbraMárcio DantasOBJECTIVES: The objectives of our study were as follows: 1) to analyze the prognostic value of macrophage infiltration in primary IgA nephropathy (IgAN) and 2) to study the relationship between macrophages and other factors associated with the development of renal fibrosis, including mast cells, TGF-β1, α-SMA and NF-kB. METHODS: We analyzed 62 patients who had been diagnosed with IgAN between 1987 and 2003. Immunohistochemical staining was performed with monoclonal antibodies against CD68 and mast cell tryptase and polyclonal antibodies against TGF-β1, α-SMA and NF-kB p65. We also used Southwestern histochemistry for the in situ detection of activated NF-kB. RESULTS: The infiltration of macrophages into the tubulointerstitial compartment correlated with unfavorable clinical and histological parameters, and a worse clinical course of IgAN was significantly associated with the number of tubulointerstitial macrophages. Kaplan-Meier curves demonstrated that increased macrophage infiltration was associated with decreased renal survival. Moreover, the presence of macrophages was associated with mast cells, tubulointerstitial α-SMA expression and NF-kB activation (IH and Southwestern histochemistry). In the multivariate analysis, the two parameters that correlated with macrophage infiltration, proteinuria and tubulointerstitial injury, were independently associated with an unfavorable clinical course. CONCLUSION: An increased number of macrophages in the tubulointerstitial area may serve as a predictive factor for poor prognosis in patients with IgAN, and these cells were also associated with the expression of pro-fibrotic factors.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012000700001IgA nephropathyMacrophageRenal fibrosisGlomerulonephritisSouthwestern immunohistochemistry |
| spellingShingle | Gyl Eanes Barros Silva Roberto Silva Costa Roberto Cuan Ravinal Leandra Naira Zambelli Ramalho Marlene Antonia dos Reis Miguel Moyses-Neto Elen Almeida Romao Terezila Machado Coimbra Márcio Dantas Renal macrophage infiltration is associated with a poor outcome in IgA nephropathy Clinics IgA nephropathy Macrophage Renal fibrosis Glomerulonephritis Southwestern immunohistochemistry |
| title | Renal macrophage infiltration is associated with a poor outcome in IgA nephropathy |
| title_full | Renal macrophage infiltration is associated with a poor outcome in IgA nephropathy |
| title_fullStr | Renal macrophage infiltration is associated with a poor outcome in IgA nephropathy |
| title_full_unstemmed | Renal macrophage infiltration is associated with a poor outcome in IgA nephropathy |
| title_short | Renal macrophage infiltration is associated with a poor outcome in IgA nephropathy |
| title_sort | renal macrophage infiltration is associated with a poor outcome in iga nephropathy |
| topic | IgA nephropathy Macrophage Renal fibrosis Glomerulonephritis Southwestern immunohistochemistry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322012000700001 |
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