Comparison of different methods for exogenous DNA uptake by bovine spermatozoa

Comparison of different methods for exogenous DNA uptake by bovine spermatozoa

Although genetic manipulation of farm animals is of great interest for animal production and the pharmaceutical industry, its efficiency remains far from satisfactory. Pronuclear injection, which is the most widely used technique for such modification, mainly in mice, remains limited for this specie...

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Bibliographic Details
Main Authors: Renata Simões, Weber Beringui Feitosa, Marcella Pecora Milazzotto, Alessandra Corallo Nicacio, Flavia Regina Oliveira de Barros, José Sergio de Arruda Gonçalves, Mariana Groke Marques, José Antônio Visintin, Mayra Elena Ortiz D'Ávila Assumpção
Format: Article
Language:English
Published: Universidade de São Paulo 2015-04-01
Series:Brazilian Journal of Veterinary Research and Animal Science
Subjects:
Spermatozoa
Cattle
Animal transgenesis
In vitro fertilization
Embryos
Online Access:http://www.revistas.usp.br/bjvras/article/view/82459

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