A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents
An animal model, suitable for resembling Parkinson's disease (PD) progress, should show both, motor and non-motor alterations. However, these features have been scarcely evaluated or developed in parkinsonian models induced by neurotoxins. This protocol provides modifications to original method...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016120300418 |
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| author | Luis O. Soto-Rojas Cecilia Bañuelos Linda Garces-Ramirez Claudia Luna-Herrera Yazmin M. Flores-Martínez Guadalupe Soto-Rodríguez Bismark Gatica-García Francisco E. López-Salas José Ayala-Dávila María E. Gutiérrez-Castillo América Padilla-Viveros Fidel de la Cruz-López Irma A. Martínez-Davila Daniel Martinez-Fong |
| author_facet | Luis O. Soto-Rojas Cecilia Bañuelos Linda Garces-Ramirez Claudia Luna-Herrera Yazmin M. Flores-Martínez Guadalupe Soto-Rodríguez Bismark Gatica-García Francisco E. López-Salas José Ayala-Dávila María E. Gutiérrez-Castillo América Padilla-Viveros Fidel de la Cruz-López Irma A. Martínez-Davila Daniel Martinez-Fong |
| author_sort | Luis O. Soto-Rojas |
| collection | DOAJ |
| description | An animal model, suitable for resembling Parkinson's disease (PD) progress, should show both, motor and non-motor alterations. However, these features have been scarcely evaluated or developed in parkinsonian models induced by neurotoxins. This protocol provides modifications to original methods, allowing six different motor and non-motor behavior tests, which adequately and timely emulate the main parkinsonian sensorimotor alterations in the rat or mouse: (1) bilateral sensorimotor alterations, examined by the vibrissae test; (2) balance and motor coordination, evaluated by the uncoordinated gait test; (3) locomotor asymmetry, analyzed by the cylinder test; (4) bradykinesia, as a locomotor alteration evidenced by the open field test; (5) depressive-like behavior, judged by the forced swimming test; and (6) hyposmia, assessed by the olfactory asymmetry test. Some advantages of using these behavioral tests over others include: • No sophisticated materials or equipment are required for their application and evaluation. • They are used in rodent models for parkinsonian research, but they can also be helpful for studying other movement disorders. • These tests can accurately discriminate the affected side from the healthy one, after unilateral injury of one hemisphere, resulting in sensorimotor, olfactory or locomotor asymmetry. |
| first_indexed | 2024-12-14T21:21:40Z |
| format | Article |
| id | doaj.art-3c146b565ba14aeab528179f90c90fa3 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-12-14T21:21:40Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-3c146b565ba14aeab528179f90c90fa32022-12-21T22:46:56ZengElsevierMethodsX2215-01612020-01-017100821A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodentsLuis O. Soto-Rojas0Cecilia Bañuelos1Linda Garces-Ramirez2Claudia Luna-Herrera3Yazmin M. Flores-Martínez4Guadalupe Soto-Rodríguez5Bismark Gatica-García6Francisco E. López-Salas7José Ayala-Dávila8María E. Gutiérrez-Castillo9América Padilla-Viveros10Fidel de la Cruz-López11Irma A. Martínez-Davila12Daniel Martinez-Fong13Departamento de Fisiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Unidad Profesional “Adolfo López Mateos”, Ciudad de México 07738, México; Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No. 1, Los Reyes Iztacala, Tlalnepantla, Edo. de México 54090, MéxicoCoordinación General de Programas Multidisciplinarios. Programa Transdisciplinario en Desarrollo Científico y Tecnológico para la Sociedad, Av. Instituto Politécnico Nacional No. 2508, Centro de Investigación y de Estudios Avanzados, Ciudad de México 07360, MéxicoDepartamento de Fisiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Unidad Profesional “Adolfo López Mateos”, Ciudad de México 07738, MéxicoDepartamento de Fisiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Unidad Profesional “Adolfo López Mateos”, Ciudad de México 07738, MéxicoDepartamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional No. 2508, Ciudad de México 07360, MéxicoFacultad de Medicina, Benemérita Universidad Autónoma de Puebla, 13 Sur 2702, Puebla 72420, MéxicoDepartamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional No. 2508, Ciudad de México 07360, MéxicoPrograma de Doctorado en Nanociencias y Nanotecnología, Av. Instituto Politécnico Nacional No. 2508, Centro de Investigación y de Estudios Avanzados, Ciudad de México 07360, MéxicoDepartamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional No. 2508, Ciudad de México 07360, MéxicoDepartamento de Biociencias e Ingeniería, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Instituto Politécnico Nacional, 30 de junio de 1520s/n, Ciudad de México 07340, MéxicoCoordinación General de Programas Multidisciplinarios. Programa Transdisciplinario en Desarrollo Científico y Tecnológico para la Sociedad, Av. Instituto Politécnico Nacional No. 2508, Centro de Investigación y de Estudios Avanzados, Ciudad de México 07360, MéxicoDepartamento de Fisiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n, Unidad Profesional “Adolfo López Mateos”, Ciudad de México 07738, MéxicoDepartamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional No. 2508, Ciudad de México 07360, MéxicoDepartamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional No. 2508, Ciudad de México 07360, México; Programa de Doctorado en Nanociencias y Nanotecnología, Av. Instituto Politécnico Nacional No. 2508, Centro de Investigación y de Estudios Avanzados, Ciudad de México 07360, México; Corresponding author at: Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional No. 2508, Ciudad de México 07360, México.An animal model, suitable for resembling Parkinson's disease (PD) progress, should show both, motor and non-motor alterations. However, these features have been scarcely evaluated or developed in parkinsonian models induced by neurotoxins. This protocol provides modifications to original methods, allowing six different motor and non-motor behavior tests, which adequately and timely emulate the main parkinsonian sensorimotor alterations in the rat or mouse: (1) bilateral sensorimotor alterations, examined by the vibrissae test; (2) balance and motor coordination, evaluated by the uncoordinated gait test; (3) locomotor asymmetry, analyzed by the cylinder test; (4) bradykinesia, as a locomotor alteration evidenced by the open field test; (5) depressive-like behavior, judged by the forced swimming test; and (6) hyposmia, assessed by the olfactory asymmetry test. Some advantages of using these behavioral tests over others include: • No sophisticated materials or equipment are required for their application and evaluation. • They are used in rodent models for parkinsonian research, but they can also be helpful for studying other movement disorders. • These tests can accurately discriminate the affected side from the healthy one, after unilateral injury of one hemisphere, resulting in sensorimotor, olfactory or locomotor asymmetry.http://www.sciencedirect.com/science/article/pii/S2215016120300418Depressive like-behaviorLocomotor asymmetryBradykinesiaSensorimotor alterationUncoordinated gaitParkinsonism |
| spellingShingle | Luis O. Soto-Rojas Cecilia Bañuelos Linda Garces-Ramirez Claudia Luna-Herrera Yazmin M. Flores-Martínez Guadalupe Soto-Rodríguez Bismark Gatica-García Francisco E. López-Salas José Ayala-Dávila María E. Gutiérrez-Castillo América Padilla-Viveros Fidel de la Cruz-López Irma A. Martínez-Davila Daniel Martinez-Fong A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents MethodsX Depressive like-behavior Locomotor asymmetry Bradykinesia Sensorimotor alteration Uncoordinated gait Parkinsonism |
| title | A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents |
| title_full | A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents |
| title_fullStr | A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents |
| title_full_unstemmed | A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents |
| title_short | A sequential methodology for integral evaluation of motor and non-motor behaviors in parkinsonian rodents |
| title_sort | sequential methodology for integral evaluation of motor and non motor behaviors in parkinsonian rodents |
| topic | Depressive like-behavior Locomotor asymmetry Bradykinesia Sensorimotor alteration Uncoordinated gait Parkinsonism |
| url | http://www.sciencedirect.com/science/article/pii/S2215016120300418 |
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