Engineering the protein dynamics of an ancestral luciferase
Directed evolution commonly relies on point mutations but InDels frequently occur in evolution. Here the authors report a protein-engineering framework based on InDel mutagenesis and fragment transplantation resulting in greater catalysis and longer glow-type bioluminescence of the ancestral lucifer...
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Bibliographic Details
| Main Authors: |
Andrea Schenkmayerova,
Gaspar P. Pinto,
Martin Toul,
Martin Marek,
Lenka Hernychova,
Joan Planas-Iglesias,
Veronika Daniel Liskova,
Daniel Pluskal,
Michal Vasina,
Stephane Emond,
Mark Dörr,
Radka Chaloupkova,
David Bednar,
Zbynek Prokop,
Florian Hollfelder,
Uwe T. Bornscheuer,
Jiri Damborsky |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Portfolio
2021-06-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-021-23450-z
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