Optimization of pre‐analytical and analytical steps for DNA and RNA analysis of fresh cytology samples

Optimization of pre‐analytical and analytical steps for DNA and RNA analysis of fresh cytology samples

Abstract Background Different cytology preparations can be used for molecular diagnostics, however the influence of pre‐analytical and analytical steps on the results are not yet well defined. We aimed to determine optimal steps for efficient extraction of DNA and RNA from fresh cells for molecular...

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Bibliographic Details
Main Authors: Ana Dolinar, Gašper Grubelnik, Irena Srebotnik‐Kirbiš, Margareta Strojan Fležar, Margareta Žlajpah
Format: Article
Language:English
Published: Wiley 2022-11-01
Series:Cancer Medicine
Subjects:
automated extraction
cytological samples
DNA
extraction methods
extraction with TRI reagent
RNA
Online Access:https://doi.org/10.1002/cam4.4728

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