Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes <i>In Vitro</i>
(1) Background: Exosomes (EXOs) have been considered a new target thought to be involved in and treat wound healing. More research is needed to fully understand EXO characteristics and the mechanisms of EXO-mediated wound healing, especially wound healing after burn injury. (2) Methods: All EXOs wer...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/12/13/1738 |
| _version_ | 1797591957871001600 |
|---|---|
| author | Jie Ding Yingying Pan Shammy Raj Lindy Schaffrick Jolene Wong Antoinette Nguyen Sharada Manchikanti Larry Unsworth Peter Kwan Edward Tredget |
| author_facet | Jie Ding Yingying Pan Shammy Raj Lindy Schaffrick Jolene Wong Antoinette Nguyen Sharada Manchikanti Larry Unsworth Peter Kwan Edward Tredget |
| author_sort | Jie Ding |
| collection | DOAJ |
| description | (1) Background: Exosomes (EXOs) have been considered a new target thought to be involved in and treat wound healing. More research is needed to fully understand EXO characteristics and the mechanisms of EXO-mediated wound healing, especially wound healing after burn injury. (2) Methods: All EXOs were isolated from 85 serum samples of 29 burn patients and 13 healthy individuals. We characterized the EXOs for morphology and density, serum concentration, protein level, marker expression, size distribution, and cytokine content. After a confirmation of EXO uptake by dermal fibroblasts, we also explored the functional regulation of primary human normal skin and hypertrophic scar fibroblast cell lines by the EXOs <i>in vitro</i>, including cell proliferation and apoptosis. (3) Results: EXOs dynamically changed their morphology, density, size, and cytokine level during wound healing in burn patients, which were correlated with burn severity and the stages of wound healing. EXOs both from burn patients and healthy individuals stimulated dermal fibroblast proliferation and apoptosis. (4) Conclusions: EXO features may be important signals that influence wound healing after burn injury; however, to understand the mechanisms by which EXOs regulates the fibroblasts in healing wounds, further studies will be required. |
| first_indexed | 2024-03-11T01:45:00Z |
| format | Article |
| id | doaj.art-3c1ed18a5485431782bb2fee7746f936 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T01:45:00Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3c1ed18a5485431782bb2fee7746f9362023-11-18T16:19:20ZengMDPI AGCells2073-44092023-06-011213173810.3390/cells12131738Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes <i>In Vitro</i>Jie Ding0Yingying Pan1Shammy Raj2Lindy Schaffrick3Jolene Wong4Antoinette Nguyen5Sharada Manchikanti6Larry Unsworth7Peter Kwan8Edward Tredget9Wound Healing Research Group, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2S2, CanadaWound Healing Research Group, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2S2, CanadaDepartment of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB T6G 2S2, CanadaWound Healing Research Group, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2S2, CanadaWound Healing Research Group, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2S2, CanadaWound Healing Research Group, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2S2, CanadaWound Healing Research Group, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2S2, CanadaDepartment of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB T6G 2S2, CanadaWound Healing Research Group, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2S2, CanadaWound Healing Research Group, Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2S2, Canada(1) Background: Exosomes (EXOs) have been considered a new target thought to be involved in and treat wound healing. More research is needed to fully understand EXO characteristics and the mechanisms of EXO-mediated wound healing, especially wound healing after burn injury. (2) Methods: All EXOs were isolated from 85 serum samples of 29 burn patients and 13 healthy individuals. We characterized the EXOs for morphology and density, serum concentration, protein level, marker expression, size distribution, and cytokine content. After a confirmation of EXO uptake by dermal fibroblasts, we also explored the functional regulation of primary human normal skin and hypertrophic scar fibroblast cell lines by the EXOs <i>in vitro</i>, including cell proliferation and apoptosis. (3) Results: EXOs dynamically changed their morphology, density, size, and cytokine level during wound healing in burn patients, which were correlated with burn severity and the stages of wound healing. EXOs both from burn patients and healthy individuals stimulated dermal fibroblast proliferation and apoptosis. (4) Conclusions: EXO features may be important signals that influence wound healing after burn injury; however, to understand the mechanisms by which EXOs regulates the fibroblasts in healing wounds, further studies will be required.https://www.mdpi.com/2073-4409/12/13/1738exosomes (EXOs)burnswound healinghypertrophic scarringcytokines |
| spellingShingle | Jie Ding Yingying Pan Shammy Raj Lindy Schaffrick Jolene Wong Antoinette Nguyen Sharada Manchikanti Larry Unsworth Peter Kwan Edward Tredget Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes <i>In Vitro</i> Cells exosomes (EXOs) burns wound healing hypertrophic scarring cytokines |
| title | Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes <i>In Vitro</i> |
| title_full | Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes <i>In Vitro</i> |
| title_fullStr | Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes <i>In Vitro</i> |
| title_full_unstemmed | Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes <i>In Vitro</i> |
| title_short | Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes <i>In Vitro</i> |
| title_sort | characteristics of serum exosomes after burn injury and dermal fibroblast regulation by exosomes i in vitro i |
| topic | exosomes (EXOs) burns wound healing hypertrophic scarring cytokines |
| url | https://www.mdpi.com/2073-4409/12/13/1738 |
| work_keys_str_mv | AT jieding characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi AT yingyingpan characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi AT shammyraj characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi AT lindyschaffrick characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi AT jolenewong characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi AT antoinettenguyen characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi AT sharadamanchikanti characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi AT larryunsworth characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi AT peterkwan characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi AT edwardtredget characteristicsofserumexosomesafterburninjuryanddermalfibroblastregulationbyexosomesiinvitroi |