Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine
Abstract Background Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom ag...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SciELO
2017-05-01
|
| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40409-017-0118-7 |
| _version_ | 1819203291198783488 |
|---|---|
| author | Ricardo Teixeira-Araújo Patrícia Castanheira Leonora Brazil-Más Francisco Pontes Moema Leitão de Araújo Maria Lucia Machado Alves Russolina Benedeta Zingali Carlos Correa-Netto |
| author_facet | Ricardo Teixeira-Araújo Patrícia Castanheira Leonora Brazil-Más Francisco Pontes Moema Leitão de Araújo Maria Lucia Machado Alves Russolina Benedeta Zingali Carlos Correa-Netto |
| author_sort | Ricardo Teixeira-Araújo |
| collection | DOAJ |
| description | Abstract Background Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom against South American rattlesnake (Crotalus durissus terrificus) bites is produced by public institutions based on the guidelines defined by the regulatory agency of the Brazilian Ministry of Health, ANVISA. However, each institution uses its own mixture of rattlesnake venom antigens. Previous works have shown that crotamine, a toxin found in Crolatus durissus venom, shows marked individual and populational variation. In addition, serum produced from crotamine-negative venoms fails to recognize this molecule. Methods In this work, we used an antivenomics approach to assess the cross-reactivity of crotalic antivenom manufactured by IVB towards crotamine-negative venom and a mixture of crotamine-negative/crotamine-positive venoms. Results We show that the venom mixture containing 20% crotamine and 57% crotoxin produced a strong immunogenic response in horses. Antivenom raised against this venom mixture reacted with most venom components including crotamine and crotoxin, in contrast to the antivenom raised against crotamine-negative venom. Conclusions These results indicate that venomic databases and antivenomics analysis provide a useful approach for choosing the better venom mixture for antibody production and for the subsequent screening of antivenom cross-reactivity with relevant snake venom components. |
| first_indexed | 2024-12-23T04:17:35Z |
| format | Article |
| id | doaj.art-3c21cf9986944e58888cfe311972b3a8 |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-23T04:17:35Z |
| publishDate | 2017-05-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-3c21cf9986944e58888cfe311972b3a82022-12-21T18:00:21ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992017-05-012311810.1186/s40409-017-0118-7Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamineRicardo Teixeira-Araújo0Patrícia Castanheira1Leonora Brazil-Más2Francisco Pontes3Moema Leitão de Araújo4Maria Lucia Machado Alves5Russolina Benedeta Zingali6Carlos Correa-Netto7Laboratório de Hemostase e Venenos, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro (UFRJ)Laboratório de Hemostase e Venenos, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro (UFRJ)Departamento de Antígenos e Cultivo Celular, Instituto Vital BrazilLaboratório de Hemostase e Venenos, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro (UFRJ)Núcleo Regional de Ofiologia de Porto Alegre (NOPA), Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do SulNúcleo Regional de Ofiologia de Porto Alegre (NOPA), Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do SulLaboratório de Hemostase e Venenos, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro (UFRJ)Laboratório de Hemostase e Venenos, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro (UFRJ)Abstract Background Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom against South American rattlesnake (Crotalus durissus terrificus) bites is produced by public institutions based on the guidelines defined by the regulatory agency of the Brazilian Ministry of Health, ANVISA. However, each institution uses its own mixture of rattlesnake venom antigens. Previous works have shown that crotamine, a toxin found in Crolatus durissus venom, shows marked individual and populational variation. In addition, serum produced from crotamine-negative venoms fails to recognize this molecule. Methods In this work, we used an antivenomics approach to assess the cross-reactivity of crotalic antivenom manufactured by IVB towards crotamine-negative venom and a mixture of crotamine-negative/crotamine-positive venoms. Results We show that the venom mixture containing 20% crotamine and 57% crotoxin produced a strong immunogenic response in horses. Antivenom raised against this venom mixture reacted with most venom components including crotamine and crotoxin, in contrast to the antivenom raised against crotamine-negative venom. Conclusions These results indicate that venomic databases and antivenomics analysis provide a useful approach for choosing the better venom mixture for antibody production and for the subsequent screening of antivenom cross-reactivity with relevant snake venom components.http://link.springer.com/article/10.1186/s40409-017-0118-7Antivenom productionAntivenomicsCrotalus durissusCrotamineCrotoxinGeographic venom variation |
| spellingShingle | Ricardo Teixeira-Araújo Patrícia Castanheira Leonora Brazil-Más Francisco Pontes Moema Leitão de Araújo Maria Lucia Machado Alves Russolina Benedeta Zingali Carlos Correa-Netto Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine Journal of Venomous Animals and Toxins including Tropical Diseases Antivenom production Antivenomics Crotalus durissus Crotamine Crotoxin Geographic venom variation |
| title | Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
| title_full | Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
| title_fullStr | Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
| title_full_unstemmed | Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
| title_short | Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
| title_sort | antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom a study with crotamine |
| topic | Antivenom production Antivenomics Crotalus durissus Crotamine Crotoxin Geographic venom variation |
| url | http://link.springer.com/article/10.1186/s40409-017-0118-7 |
| work_keys_str_mv | AT ricardoteixeiraaraujo antivenomicsasatooltoimprovetheneutralizingcapacityofthecrotalicantivenomastudywithcrotamine AT patriciacastanheira antivenomicsasatooltoimprovetheneutralizingcapacityofthecrotalicantivenomastudywithcrotamine AT leonorabrazilmas antivenomicsasatooltoimprovetheneutralizingcapacityofthecrotalicantivenomastudywithcrotamine AT franciscopontes antivenomicsasatooltoimprovetheneutralizingcapacityofthecrotalicantivenomastudywithcrotamine AT moemaleitaodearaujo antivenomicsasatooltoimprovetheneutralizingcapacityofthecrotalicantivenomastudywithcrotamine AT marialuciamachadoalves antivenomicsasatooltoimprovetheneutralizingcapacityofthecrotalicantivenomastudywithcrotamine AT russolinabenedetazingali antivenomicsasatooltoimprovetheneutralizingcapacityofthecrotalicantivenomastudywithcrotamine AT carloscorreanetto antivenomicsasatooltoimprovetheneutralizingcapacityofthecrotalicantivenomastudywithcrotamine |