<b>Effect of pain related of sex and estrous cycle on blood plasma glucose, free fatty acids and corticosterone in rats</b> - doi: 10.4025/actascibiolsci.v34i4.10568
Sex differences related with pain have been studied and evidences suggesting influence of sex steroid hormones on the thresholds of pain. Experimental nociception has been test using formalin as a model of nociceptive stimulus. Association of stress, pain and metabolic and hormonal changes has not b...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2012-03-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/10568 |
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| author | Elzira Diniz de Moraes Cássia Thaís Bussamra Vieira Zaia Dimas Augusto Morozin Zaia Angelo Alexander Torres dos Santos Rachel Cezar de Andrade Ribeiro Andreia Lopes da Silva |
| author_facet | Elzira Diniz de Moraes Cássia Thaís Bussamra Vieira Zaia Dimas Augusto Morozin Zaia Angelo Alexander Torres dos Santos Rachel Cezar de Andrade Ribeiro Andreia Lopes da Silva |
| author_sort | Elzira Diniz de Moraes |
| collection | DOAJ |
| description | Sex differences related with pain have been studied and evidences suggesting influence of sex steroid hormones on the thresholds of pain. Experimental nociception has been test using formalin as a model of nociceptive stimulus. Association of stress, pain and metabolic and hormonal changes has not been explored. The aim of this study was to compare metabolic and hormonal changes between male rats and female rats in proestrus and estrus cycle after painful stimulus by formalin into the masseter muscle. Male and female Wistar rats (200-250 g b.w.) were submitted to an injection of formalin (F, 1.5%) or saline (S, 9.9%) into the masseter muscle and after 0 (N, control group without injection), 5, 15, 30 or 60 minutes they were decapitate and blood was collected to measure biochemical parameters. Plasma estradiol concentration (pg dL-1) was significantly higher in proestrus (106.3 ± 4.3, n = 45, p < 0.05) group compared to the estrous group (89.4 ± 3.5, n = 43). Blood plasma concentration of glucose (mg dL-1) was increased after 5 and 15 minutes of injection of formalin or saline in the animals, but in the estrus group the increase was bigger than in the others. Free fatty acids levels increased in the estrous group after 5, 15 and 30 minutes and also the corticosterone levels and these concentrations wer significantly different (p < 0.05) from either male or female animals in proestrus state. The results obtained suggesting that estradiol is related to a sensibility to pain and the estrus stage is related to stress and the estrous cycle has a modulator effect on pain and nociceptive sensibility.
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| first_indexed | 2024-04-13T14:47:52Z |
| format | Article |
| id | doaj.art-3c22911658224025b4fa1569834eaae4 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-13T14:47:52Z |
| publishDate | 2012-03-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-3c22911658224025b4fa1569834eaae42022-12-22T02:42:41ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2012-03-0134410.4025/actascibiolsci.v34i4.10568<b>Effect of pain related of sex and estrous cycle on blood plasma glucose, free fatty acids and corticosterone in rats</b> - doi: 10.4025/actascibiolsci.v34i4.10568Elzira Diniz de Moraes0Cássia Thaís Bussamra Vieira Zaia1Dimas Augusto Morozin Zaia2Angelo Alexander Torres dos Santos3Rachel Cezar de Andrade Ribeiro4Andreia Lopes da Silva5Instituto de Ensino Superior de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaSex differences related with pain have been studied and evidences suggesting influence of sex steroid hormones on the thresholds of pain. Experimental nociception has been test using formalin as a model of nociceptive stimulus. Association of stress, pain and metabolic and hormonal changes has not been explored. The aim of this study was to compare metabolic and hormonal changes between male rats and female rats in proestrus and estrus cycle after painful stimulus by formalin into the masseter muscle. Male and female Wistar rats (200-250 g b.w.) were submitted to an injection of formalin (F, 1.5%) or saline (S, 9.9%) into the masseter muscle and after 0 (N, control group without injection), 5, 15, 30 or 60 minutes they were decapitate and blood was collected to measure biochemical parameters. Plasma estradiol concentration (pg dL-1) was significantly higher in proestrus (106.3 ± 4.3, n = 45, p < 0.05) group compared to the estrous group (89.4 ± 3.5, n = 43). Blood plasma concentration of glucose (mg dL-1) was increased after 5 and 15 minutes of injection of formalin or saline in the animals, but in the estrus group the increase was bigger than in the others. Free fatty acids levels increased in the estrous group after 5, 15 and 30 minutes and also the corticosterone levels and these concentrations wer significantly different (p < 0.05) from either male or female animals in proestrus state. The results obtained suggesting that estradiol is related to a sensibility to pain and the estrus stage is related to stress and the estrous cycle has a modulator effect on pain and nociceptive sensibility. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/10568sex differencesnociceptionstresshormonesrats |
| spellingShingle | Elzira Diniz de Moraes Cássia Thaís Bussamra Vieira Zaia Dimas Augusto Morozin Zaia Angelo Alexander Torres dos Santos Rachel Cezar de Andrade Ribeiro Andreia Lopes da Silva <b>Effect of pain related of sex and estrous cycle on blood plasma glucose, free fatty acids and corticosterone in rats</b> - doi: 10.4025/actascibiolsci.v34i4.10568 Acta Scientiarum: Biological Sciences sex differences nociception stress hormones rats |
| title | <b>Effect of pain related of sex and estrous cycle on blood plasma glucose, free fatty acids and corticosterone in rats</b> - doi: 10.4025/actascibiolsci.v34i4.10568 |
| title_full | <b>Effect of pain related of sex and estrous cycle on blood plasma glucose, free fatty acids and corticosterone in rats</b> - doi: 10.4025/actascibiolsci.v34i4.10568 |
| title_fullStr | <b>Effect of pain related of sex and estrous cycle on blood plasma glucose, free fatty acids and corticosterone in rats</b> - doi: 10.4025/actascibiolsci.v34i4.10568 |
| title_full_unstemmed | <b>Effect of pain related of sex and estrous cycle on blood plasma glucose, free fatty acids and corticosterone in rats</b> - doi: 10.4025/actascibiolsci.v34i4.10568 |
| title_short | <b>Effect of pain related of sex and estrous cycle on blood plasma glucose, free fatty acids and corticosterone in rats</b> - doi: 10.4025/actascibiolsci.v34i4.10568 |
| title_sort | b effect of pain related of sex and estrous cycle on blood plasma glucose free fatty acids and corticosterone in rats b doi 10 4025 actascibiolsci v34i4 10568 |
| topic | sex differences nociception stress hormones rats |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/10568 |
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