Opportunity and challenges for nanotechnology application for genome editing in plants
abstract: CRISPR-Cas9 genome editing systems have enormous promise in the areas of biomedical research and agriculture. While the effectiveness and utility of CRISPR have already been well established, there are many challenges in the successful application of CRISPR to plant systems. In this regard...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Plant Nano Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773111122000018 |
| _version_ | 1827374861925744640 |
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| author | Sanskriti Vats Surbhi Kumawat Jashandeep Brar Sukhmandeep Kaur Karmveer Yadav Sayali G. Magar Pravin V. Jadhav Prafull Salvi Humira Sonah Sandhya Sharma Rupesh Deshmukh |
| author_facet | Sanskriti Vats Surbhi Kumawat Jashandeep Brar Sukhmandeep Kaur Karmveer Yadav Sayali G. Magar Pravin V. Jadhav Prafull Salvi Humira Sonah Sandhya Sharma Rupesh Deshmukh |
| author_sort | Sanskriti Vats |
| collection | DOAJ |
| description | abstract: CRISPR-Cas9 genome editing systems have enormous promise in the areas of biomedical research and agriculture. While the effectiveness and utility of CRISPR have already been well established, there are many challenges in the successful application of CRISPR to plant systems. In this regard, nanotechnology could play a critical role in addressing some of the decisive challenges preventing CRISPR-mediated genome engineering in plants especially in recalcitrant species. Nanoparticles can be used as an efficient delivery agent to the targeted cell and enhance genome editing efficiency. The Nanoparticles-based delivery system can deliver functional gene, RNP, or siRNA intact into the plant cell resulting in the generation of genome-edited plants without transgenes. In this review, we have discussed various nanoparticle technologies that can overcome barriers associated with the CRISPR-mediated genetic transformation in plants, holding it to reach its full potential. CRISPR can be used to develop tolerance against various biotic and abiotic stresses. As well as, it may also be employed in improving food quality and productivity thereby providing food security to the ever-growing human population. |
| first_indexed | 2024-03-08T11:41:10Z |
| format | Article |
| id | doaj.art-3c2830753fb241eeafad6acd6d6c5db2 |
| institution | Directory Open Access Journal |
| issn | 2773-1111 |
| language | English |
| last_indexed | 2024-03-08T11:41:10Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Nano Biology |
| spelling | doaj.art-3c2830753fb241eeafad6acd6d6c5db22024-01-25T05:24:40ZengElsevierPlant Nano Biology2773-11112022-06-011100001Opportunity and challenges for nanotechnology application for genome editing in plantsSanskriti Vats0Surbhi Kumawat1Jashandeep Brar2Sukhmandeep Kaur3Karmveer Yadav4Sayali G. Magar5Pravin V. Jadhav6Prafull Salvi7Humira Sonah8Sandhya Sharma9Rupesh Deshmukh10National Agri-Food Biotechnology Institute (NABI) Mohali, Punjab, India; Regional Centre for Biotechnology, Faridabad, Haryana (NCR Delhi), IndiaNational Agri-Food Biotechnology Institute (NABI) Mohali, Punjab, India; Department of Biotechnology, Panjab University, Chandigarh, IndiaNational Agri-Food Biotechnology Institute (NABI) Mohali, Punjab, IndiaNational Agri-Food Biotechnology Institute (NABI) Mohali, Punjab, India; Department of Biotechnology, Panjab University, Chandigarh, IndiaICAR-National Dairy Research Institute, Karnal, Haryana, IndiaBiotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra, IndiaBiotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra, IndiaNational Agri-Food Biotechnology Institute (NABI) Mohali, Punjab, IndiaNational Agri-Food Biotechnology Institute (NABI) Mohali, Punjab, IndiaICAR-National Institute for Plant Biotechnology, New Delhi, India; Correspondence to: ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110012 India.National Agri-Food Biotechnology Institute (NABI) Mohali, Punjab, India; Correspondence to: National Agri-Food Biotechnology Institute (NABI), Mohali, Punjab 140306, India.abstract: CRISPR-Cas9 genome editing systems have enormous promise in the areas of biomedical research and agriculture. While the effectiveness and utility of CRISPR have already been well established, there are many challenges in the successful application of CRISPR to plant systems. In this regard, nanotechnology could play a critical role in addressing some of the decisive challenges preventing CRISPR-mediated genome engineering in plants especially in recalcitrant species. Nanoparticles can be used as an efficient delivery agent to the targeted cell and enhance genome editing efficiency. The Nanoparticles-based delivery system can deliver functional gene, RNP, or siRNA intact into the plant cell resulting in the generation of genome-edited plants without transgenes. In this review, we have discussed various nanoparticle technologies that can overcome barriers associated with the CRISPR-mediated genetic transformation in plants, holding it to reach its full potential. CRISPR can be used to develop tolerance against various biotic and abiotic stresses. As well as, it may also be employed in improving food quality and productivity thereby providing food security to the ever-growing human population.http://www.sciencedirect.com/science/article/pii/S2773111122000018NanoparticleMagnetofectionDelivery methodGenome editingCRISPR |
| spellingShingle | Sanskriti Vats Surbhi Kumawat Jashandeep Brar Sukhmandeep Kaur Karmveer Yadav Sayali G. Magar Pravin V. Jadhav Prafull Salvi Humira Sonah Sandhya Sharma Rupesh Deshmukh Opportunity and challenges for nanotechnology application for genome editing in plants Plant Nano Biology Nanoparticle Magnetofection Delivery method Genome editing CRISPR |
| title | Opportunity and challenges for nanotechnology application for genome editing in plants |
| title_full | Opportunity and challenges for nanotechnology application for genome editing in plants |
| title_fullStr | Opportunity and challenges for nanotechnology application for genome editing in plants |
| title_full_unstemmed | Opportunity and challenges for nanotechnology application for genome editing in plants |
| title_short | Opportunity and challenges for nanotechnology application for genome editing in plants |
| title_sort | opportunity and challenges for nanotechnology application for genome editing in plants |
| topic | Nanoparticle Magnetofection Delivery method Genome editing CRISPR |
| url | http://www.sciencedirect.com/science/article/pii/S2773111122000018 |
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