24 hours in the life of HIV-1 in a T cell line.
HIV-1 infects CD4+ T cells and completes its replication cycle in approximately 24 hours. We employed repeated measurements in a standardized cell system and rigorous mathematical modeling to characterize the emergence of the viral replication intermediates and their impact on the cellular transcrip...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC3561177?pdf=render |
| _version_ | 1818096710649380864 |
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| author | Pejman Mohammadi Sébastien Desfarges István Bartha Beda Joos Nadine Zangger Miguel Muñoz Huldrych F Günthard Niko Beerenwinkel Amalio Telenti Angela Ciuffi |
| author_facet | Pejman Mohammadi Sébastien Desfarges István Bartha Beda Joos Nadine Zangger Miguel Muñoz Huldrych F Günthard Niko Beerenwinkel Amalio Telenti Angela Ciuffi |
| author_sort | Pejman Mohammadi |
| collection | DOAJ |
| description | HIV-1 infects CD4+ T cells and completes its replication cycle in approximately 24 hours. We employed repeated measurements in a standardized cell system and rigorous mathematical modeling to characterize the emergence of the viral replication intermediates and their impact on the cellular transcriptional response with high temporal resolution. We observed 7,991 (73%) of the 10,958 expressed genes to be modulated in concordance with key steps of viral replication. Fifty-two percent of the overall variability in the host transcriptome was explained by linear regression on the viral life cycle. This profound perturbation of cellular physiology was investigated in the light of several regulatory mechanisms, including transcription factors, miRNAs, host-pathogen interaction, and proviral integration. Key features were validated in primary CD4+ T cells, and with viral constructs using alternative entry strategies. We propose a model of early massive cellular shutdown and progressive upregulation of the cellular machinery to complete the viral life cycle. |
| first_indexed | 2024-12-10T23:08:57Z |
| format | Article |
| id | doaj.art-3c2a7340926c4c58824cb6570af54114 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-10T23:08:57Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-3c2a7340926c4c58824cb6570af541142022-12-22T01:30:00ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742013-01-0191e100316110.1371/journal.ppat.100316124 hours in the life of HIV-1 in a T cell line.Pejman MohammadiSébastien DesfargesIstván BarthaBeda JoosNadine ZanggerMiguel MuñozHuldrych F GünthardNiko BeerenwinkelAmalio TelentiAngela CiuffiHIV-1 infects CD4+ T cells and completes its replication cycle in approximately 24 hours. We employed repeated measurements in a standardized cell system and rigorous mathematical modeling to characterize the emergence of the viral replication intermediates and their impact on the cellular transcriptional response with high temporal resolution. We observed 7,991 (73%) of the 10,958 expressed genes to be modulated in concordance with key steps of viral replication. Fifty-two percent of the overall variability in the host transcriptome was explained by linear regression on the viral life cycle. This profound perturbation of cellular physiology was investigated in the light of several regulatory mechanisms, including transcription factors, miRNAs, host-pathogen interaction, and proviral integration. Key features were validated in primary CD4+ T cells, and with viral constructs using alternative entry strategies. We propose a model of early massive cellular shutdown and progressive upregulation of the cellular machinery to complete the viral life cycle.http://europepmc.org/articles/PMC3561177?pdf=render |
| spellingShingle | Pejman Mohammadi Sébastien Desfarges István Bartha Beda Joos Nadine Zangger Miguel Muñoz Huldrych F Günthard Niko Beerenwinkel Amalio Telenti Angela Ciuffi 24 hours in the life of HIV-1 in a T cell line. PLoS Pathogens |
| title | 24 hours in the life of HIV-1 in a T cell line. |
| title_full | 24 hours in the life of HIV-1 in a T cell line. |
| title_fullStr | 24 hours in the life of HIV-1 in a T cell line. |
| title_full_unstemmed | 24 hours in the life of HIV-1 in a T cell line. |
| title_short | 24 hours in the life of HIV-1 in a T cell line. |
| title_sort | 24 hours in the life of hiv 1 in a t cell line |
| url | http://europepmc.org/articles/PMC3561177?pdf=render |
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