Chloroplast Hsp70 Isoform Is Required for Age-Dependent Tissue Preference of Bamboo mosaic virus in Mature Nicotiana benthamiana Leaves
Plant viruses may exhibit age-dependent tissue preference in their hosts but the underlying mechanisms are not well understood. In this study, we provide several lines of evidence to reveal the determining role of a protein of the Nicotiana benthamiana chloroplast Hsp70 (NbcpHsp70) family, NbcpHsp70...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2017-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-17-0012-R |
| _version_ | 1811291195892039680 |
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| author | Ying Wen Huang Chung Chi Hu Ching Hsiu Tsai Na Sheng Lin Yau Heiu Hsu |
| author_facet | Ying Wen Huang Chung Chi Hu Ching Hsiu Tsai Na Sheng Lin Yau Heiu Hsu |
| author_sort | Ying Wen Huang |
| collection | DOAJ |
| description | Plant viruses may exhibit age-dependent tissue preference in their hosts but the underlying mechanisms are not well understood. In this study, we provide several lines of evidence to reveal the determining role of a protein of the Nicotiana benthamiana chloroplast Hsp70 (NbcpHsp70) family, NbcpHsp70-2, involved in the preference of Bamboo mosaic virus (BaMV) to infect older tissues. NbcpHsp70 family proteins were identified in complexes pulled down with BaMV replicase as the bait. Among the isoforms of NbcpHsp70, only the specific silencing of NbcpHsp70-2 resulted in the significant decrease of BaMV RNA in N. benthamiana protopalsts, indicating that NbcpHsp70-2 is involved in the efficient replication of BaMV RNA. We further identified the age-dependent import regulation signal contained in the transit peptide of NbcpHsp70-2. Deletion, overexpression, and substitution experiments revealed that the signal in the transit peptide of NbcpHsp70-2 is crucial for both the import of NbcpHsp70-2 into older chloroplasts and the preference of BaMV for infecting older leaves of N. benthamiana. Together, these data demonstrated that BaMV may exploit a cellular age-dependent transportation mechanism to target a suitable environment for viral replication. |
| first_indexed | 2024-04-13T04:25:45Z |
| format | Article |
| id | doaj.art-3c2b137620074b1abd1d9b110bf523ab |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:25:45Z |
| publishDate | 2017-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-3c2b137620074b1abd1d9b110bf523ab2022-12-22T03:02:32ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062017-08-0130863164510.1094/MPMI-01-17-0012-RChloroplast Hsp70 Isoform Is Required for Age-Dependent Tissue Preference of Bamboo mosaic virus in Mature Nicotiana benthamiana LeavesYing Wen Huang0Chung Chi Hu1Ching Hsiu Tsai2Na Sheng Lin3Yau Heiu Hsu4Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan; andGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan; andGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan; andGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan; andGraduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan; andPlant viruses may exhibit age-dependent tissue preference in their hosts but the underlying mechanisms are not well understood. In this study, we provide several lines of evidence to reveal the determining role of a protein of the Nicotiana benthamiana chloroplast Hsp70 (NbcpHsp70) family, NbcpHsp70-2, involved in the preference of Bamboo mosaic virus (BaMV) to infect older tissues. NbcpHsp70 family proteins were identified in complexes pulled down with BaMV replicase as the bait. Among the isoforms of NbcpHsp70, only the specific silencing of NbcpHsp70-2 resulted in the significant decrease of BaMV RNA in N. benthamiana protopalsts, indicating that NbcpHsp70-2 is involved in the efficient replication of BaMV RNA. We further identified the age-dependent import regulation signal contained in the transit peptide of NbcpHsp70-2. Deletion, overexpression, and substitution experiments revealed that the signal in the transit peptide of NbcpHsp70-2 is crucial for both the import of NbcpHsp70-2 into older chloroplasts and the preference of BaMV for infecting older leaves of N. benthamiana. Together, these data demonstrated that BaMV may exploit a cellular age-dependent transportation mechanism to target a suitable environment for viral replication.https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-17-0012-R |
| spellingShingle | Ying Wen Huang Chung Chi Hu Ching Hsiu Tsai Na Sheng Lin Yau Heiu Hsu Chloroplast Hsp70 Isoform Is Required for Age-Dependent Tissue Preference of Bamboo mosaic virus in Mature Nicotiana benthamiana Leaves Molecular Plant-Microbe Interactions |
| title | Chloroplast Hsp70 Isoform Is Required for Age-Dependent Tissue Preference of Bamboo mosaic virus in Mature Nicotiana benthamiana Leaves |
| title_full | Chloroplast Hsp70 Isoform Is Required for Age-Dependent Tissue Preference of Bamboo mosaic virus in Mature Nicotiana benthamiana Leaves |
| title_fullStr | Chloroplast Hsp70 Isoform Is Required for Age-Dependent Tissue Preference of Bamboo mosaic virus in Mature Nicotiana benthamiana Leaves |
| title_full_unstemmed | Chloroplast Hsp70 Isoform Is Required for Age-Dependent Tissue Preference of Bamboo mosaic virus in Mature Nicotiana benthamiana Leaves |
| title_short | Chloroplast Hsp70 Isoform Is Required for Age-Dependent Tissue Preference of Bamboo mosaic virus in Mature Nicotiana benthamiana Leaves |
| title_sort | chloroplast hsp70 isoform is required for age dependent tissue preference of bamboo mosaic virus in mature nicotiana benthamiana leaves |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-01-17-0012-R |
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