SRAP analysis of the genetic diversity of wild castor (Ricinus communis L.) in South China.
Castor bean is an important seed oil crop. Castor oil is a highly demanded oil for several industrial uses. Currently, castor bean varieties suffer from low productivity and high risk of insect pests and diseases. It is in urgent need to mine elite genes from wild materials for castor breeding. 29 p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0219667 |
| _version_ | 1797660702656167936 |
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| author | Kwadwo Gyapong Agyenim-Boateng Jiannong Lu Yuzhen Shi Dan Zhang Xuegui Yin |
| author_facet | Kwadwo Gyapong Agyenim-Boateng Jiannong Lu Yuzhen Shi Dan Zhang Xuegui Yin |
| author_sort | Kwadwo Gyapong Agyenim-Boateng |
| collection | DOAJ |
| description | Castor bean is an important seed oil crop. Castor oil is a highly demanded oil for several industrial uses. Currently, castor bean varieties suffer from low productivity and high risk of insect pests and diseases. It is in urgent need to mine elite genes from wild materials for castor breeding. 29 pairs of polymorphic SRAP primers out of 361 pairs were used to analyse the genetic diversity of 473 wild castor materials from South China. 203 bands were amplified by the 29 pairs of primers, of which 169 bands were polymorphic, with a polymorphic percentage of 83.25%. With an average number of alleles per locus (Ap) of 1.801, average number of effective alleles per locus (Ae) of 1.713 and average percentage of polymorphic loci (P) of 90.04%, these primers were proven to be useful and effective. Nei' genetic distance between the materials ranged from 1.04 to 25.02, with an average of 13.03. At the genetic distance of 25.02, the materials clustered into two major groups, consistent with the result of population structure analysis. However, more subgroups existed between 5.21 and 13.32. Although not all the materials from the same region were clustered in the same group, an obvious trend existed where the groups were related to regions to a great extent. Based on multiple indices, the genetic diversity of materials from Hainan was the lowest. However, there was not much difference between West Guangdong and Guangxi, although the former was slightly higher. Moderate genetic differentiation was observed in wild materials in South China. The genetic differentiation mainly occurred within population, with maximum differentiation in Guangxi, followed by West Guangdong and the minimum in Hainan. Nonetheless, there was an extensive geneflow between populations. The above results provided a direction for the conservation and breeding application of these materials. |
| first_indexed | 2024-03-11T18:34:48Z |
| format | Article |
| id | doaj.art-3c2c4148622f486facc11576589cae65 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-11T18:34:48Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3c2c4148622f486facc11576589cae652023-10-13T05:31:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01147e021966710.1371/journal.pone.0219667SRAP analysis of the genetic diversity of wild castor (Ricinus communis L.) in South China.Kwadwo Gyapong Agyenim-BoatengJiannong LuYuzhen ShiDan ZhangXuegui YinCastor bean is an important seed oil crop. Castor oil is a highly demanded oil for several industrial uses. Currently, castor bean varieties suffer from low productivity and high risk of insect pests and diseases. It is in urgent need to mine elite genes from wild materials for castor breeding. 29 pairs of polymorphic SRAP primers out of 361 pairs were used to analyse the genetic diversity of 473 wild castor materials from South China. 203 bands were amplified by the 29 pairs of primers, of which 169 bands were polymorphic, with a polymorphic percentage of 83.25%. With an average number of alleles per locus (Ap) of 1.801, average number of effective alleles per locus (Ae) of 1.713 and average percentage of polymorphic loci (P) of 90.04%, these primers were proven to be useful and effective. Nei' genetic distance between the materials ranged from 1.04 to 25.02, with an average of 13.03. At the genetic distance of 25.02, the materials clustered into two major groups, consistent with the result of population structure analysis. However, more subgroups existed between 5.21 and 13.32. Although not all the materials from the same region were clustered in the same group, an obvious trend existed where the groups were related to regions to a great extent. Based on multiple indices, the genetic diversity of materials from Hainan was the lowest. However, there was not much difference between West Guangdong and Guangxi, although the former was slightly higher. Moderate genetic differentiation was observed in wild materials in South China. The genetic differentiation mainly occurred within population, with maximum differentiation in Guangxi, followed by West Guangdong and the minimum in Hainan. Nonetheless, there was an extensive geneflow between populations. The above results provided a direction for the conservation and breeding application of these materials.https://doi.org/10.1371/journal.pone.0219667 |
| spellingShingle | Kwadwo Gyapong Agyenim-Boateng Jiannong Lu Yuzhen Shi Dan Zhang Xuegui Yin SRAP analysis of the genetic diversity of wild castor (Ricinus communis L.) in South China. PLoS ONE |
| title | SRAP analysis of the genetic diversity of wild castor (Ricinus communis L.) in South China. |
| title_full | SRAP analysis of the genetic diversity of wild castor (Ricinus communis L.) in South China. |
| title_fullStr | SRAP analysis of the genetic diversity of wild castor (Ricinus communis L.) in South China. |
| title_full_unstemmed | SRAP analysis of the genetic diversity of wild castor (Ricinus communis L.) in South China. |
| title_short | SRAP analysis of the genetic diversity of wild castor (Ricinus communis L.) in South China. |
| title_sort | srap analysis of the genetic diversity of wild castor ricinus communis l in south china |
| url | https://doi.org/10.1371/journal.pone.0219667 |
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