An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035
We presented an approach to address cancer cell chemotaxis and response to tyrosine kinase inhibitor PD153035 at the single-cell level. We applied an optical tweezer system together with the platform at the single-cell level to manipulate an epidermal growth factor (EGF)-coated bead positioned close...
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MDPI AG
2021-11-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/8/12/533 |
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| author | Pei-Wen Peng Jen-Chang Yang Mamadi M.S Colley Tzu-Sen Yang |
| author_facet | Pei-Wen Peng Jen-Chang Yang Mamadi M.S Colley Tzu-Sen Yang |
| author_sort | Pei-Wen Peng |
| collection | DOAJ |
| description | We presented an approach to address cancer cell chemotaxis and response to tyrosine kinase inhibitor PD153035 at the single-cell level. We applied an optical tweezer system together with the platform at the single-cell level to manipulate an epidermal growth factor (EGF)-coated bead positioned close to the filopodia to locally stimulate HT29 cells, the human colon cancer cell line overexpressing the EGF receptor (EGFR). To address cancer cell chemotaxis, a single-cell movement model was also proposed to quantify the propagation speed at the leading and trailing edges of the cell along the chemosensing axis. This study focused on three perspectives: probing the chemosensing process mediated by EGF/EGFR signaling, investigating the mode of locomotion during the EGF-coated bead stimulation, and quantifying the effect of PD153035 on the EGF–EGFR transport pathway. The results showed that the filopodial actin filament is a sensory system for EGF detection. In addition, HT29 cells may use the filopodial actin filament to distinguish the presence or absence of the chemoattractant EGF. Furthermore, we demonstrated the high selectivity of PD153035 for EGFR and the reversibility of binding to EGFR. We anticipate that the proposed single-cell method could be applied to construct a rapid screening method for the detection and therapeutic evaluation of many types of cancer during chemotaxis. |
| first_indexed | 2024-03-10T03:18:16Z |
| format | Article |
| id | doaj.art-3c2f35913e2a4afcb5ae17921ae224f2 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-10T03:18:16Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-3c2f35913e2a4afcb5ae17921ae224f22023-11-23T10:08:30ZengMDPI AGPhotonics2304-67322021-11-0181253310.3390/photonics8120533An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035Pei-Wen Peng0Jen-Chang Yang1Mamadi M.S Colley2Tzu-Sen Yang3School of Dental Technology, Taipei Medical University, Taipei 110, TaiwanGraduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 11031, TaiwanGraduate Institute of Biomedical Optomechatronics, Taipei Medical University, Taipei 110, TaiwanSchool of Dental Technology, Taipei Medical University, Taipei 110, TaiwanWe presented an approach to address cancer cell chemotaxis and response to tyrosine kinase inhibitor PD153035 at the single-cell level. We applied an optical tweezer system together with the platform at the single-cell level to manipulate an epidermal growth factor (EGF)-coated bead positioned close to the filopodia to locally stimulate HT29 cells, the human colon cancer cell line overexpressing the EGF receptor (EGFR). To address cancer cell chemotaxis, a single-cell movement model was also proposed to quantify the propagation speed at the leading and trailing edges of the cell along the chemosensing axis. This study focused on three perspectives: probing the chemosensing process mediated by EGF/EGFR signaling, investigating the mode of locomotion during the EGF-coated bead stimulation, and quantifying the effect of PD153035 on the EGF–EGFR transport pathway. The results showed that the filopodial actin filament is a sensory system for EGF detection. In addition, HT29 cells may use the filopodial actin filament to distinguish the presence or absence of the chemoattractant EGF. Furthermore, we demonstrated the high selectivity of PD153035 for EGFR and the reversibility of binding to EGFR. We anticipate that the proposed single-cell method could be applied to construct a rapid screening method for the detection and therapeutic evaluation of many types of cancer during chemotaxis.https://www.mdpi.com/2304-6732/8/12/533chemotaxisepidermal growth factor (EGF)epidermal growth factor receptor (EGFR)tyrosine kinase inhibitorPD153035optical tweezers |
| spellingShingle | Pei-Wen Peng Jen-Chang Yang Mamadi M.S Colley Tzu-Sen Yang An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035 Photonics chemotaxis epidermal growth factor (EGF) epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor PD153035 optical tweezers |
| title | An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035 |
| title_full | An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035 |
| title_fullStr | An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035 |
| title_full_unstemmed | An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035 |
| title_short | An Optical Tweezers-Based Single-Cell Manipulation and Detection Platform for Probing Real-Time Cancer Cell Chemotaxis and Response to Tyrosine Kinase Inhibitor PD153035 |
| title_sort | optical tweezers based single cell manipulation and detection platform for probing real time cancer cell chemotaxis and response to tyrosine kinase inhibitor pd153035 |
| topic | chemotaxis epidermal growth factor (EGF) epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor PD153035 optical tweezers |
| url | https://www.mdpi.com/2304-6732/8/12/533 |
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