Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis
Background: Activated inflammatory cells produce reactive oxygen species (ROS) to eliminate pathogens. Under normal conditions, the pathogens are taken care of, and tissues are repaired. However, in periodontal disease, persistent inflammation causes increased ROS release and impaired healing. Ther...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Journal of Indian Society of Periodontology |
| Subjects: | |
| Online Access: | http://www.jisponline.com/article.asp?issn=0972-124X;year=2023;volume=27;issue=3;spage=278;epage=282;aulast=Bhatt |
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| author | Akanksha Bhatt Aarati Nayak Kishore Bhat Chetana Bogar Ranganath Nayak Sachita Naik |
| author_facet | Akanksha Bhatt Aarati Nayak Kishore Bhat Chetana Bogar Ranganath Nayak Sachita Naik |
| author_sort | Akanksha Bhatt |
| collection | DOAJ |
| description | Background: Activated inflammatory cells produce reactive oxygen species (ROS) to eliminate pathogens. Under normal conditions, the pathogens are taken care of, and tissues are repaired. However, in periodontal disease, persistent inflammation causes increased ROS release and impaired healing. Therefore, removal of overproduced ROS using antioxidants is necessary. Hydrogen water has an antioxidative effect on cells and impedes oxidative stress-related disorders. Aim: To study the effect of hydrogen water on cell viability, migration, and its antioxidative potential in fibroblasts obtained from chronic periodontitis patients. Materials and Methods: The gingival tissue samples were obtained from 26 subjects (13 periodontally healthy individuals and 13 chronic periodontitis patients) and processed. The human gingival fibroblasts were cultured and the assays were commenced once adequate growth was detected. The effect of hydrogen water on cell viability was checked by neutral red assay, while the migration potential was assessed by transwell migration assay. The antioxidative potential of hydrogen water was evaluated by CUPRAC assay. Statistical Analysis: Intergroup comparison was done using Mann–Whitney U-test. Intragroup comparison was done using Wilcoxon signed-rank test. Results: Hydrogen water was nontoxic to the fibroblasts at 24 h and 48 h. The intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (P = 0.00) difference at 24 h and 48 h. Hydrogen water also positively influenced the migratory capacity. Hydrogen water-treated fibroblasts obtained from chronic periodontitis patients showed more migration in comparison to the healthy group (P = 0.00). Hydrogen water showed an antioxidative potential. The maximum potential was seen in relation to the fibroblasts obtained from chronic periodontitis patients at 48 h. Conclusion: Hydrogen water was nontoxic, increased the migratory capacity, and showed an antioxidative potential on human fibroblasts obtained from periodontally healthy individuals and patients with chronic periodontitis. |
| first_indexed | 2024-03-12T22:11:43Z |
| format | Article |
| id | doaj.art-3c2f55adbfea40019be10b43a9b762b9 |
| institution | Directory Open Access Journal |
| issn | 0972-124X |
| language | English |
| last_indexed | 2024-03-12T22:11:43Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Society of Periodontology |
| spelling | doaj.art-3c2f55adbfea40019be10b43a9b762b92023-07-23T11:33:52ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X2023-01-0127327828210.4103/jisp.jisp_546_21Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitisAkanksha BhattAarati NayakKishore BhatChetana BogarRanganath NayakSachita NaikBackground: Activated inflammatory cells produce reactive oxygen species (ROS) to eliminate pathogens. Under normal conditions, the pathogens are taken care of, and tissues are repaired. However, in periodontal disease, persistent inflammation causes increased ROS release and impaired healing. Therefore, removal of overproduced ROS using antioxidants is necessary. Hydrogen water has an antioxidative effect on cells and impedes oxidative stress-related disorders. Aim: To study the effect of hydrogen water on cell viability, migration, and its antioxidative potential in fibroblasts obtained from chronic periodontitis patients. Materials and Methods: The gingival tissue samples were obtained from 26 subjects (13 periodontally healthy individuals and 13 chronic periodontitis patients) and processed. The human gingival fibroblasts were cultured and the assays were commenced once adequate growth was detected. The effect of hydrogen water on cell viability was checked by neutral red assay, while the migration potential was assessed by transwell migration assay. The antioxidative potential of hydrogen water was evaluated by CUPRAC assay. Statistical Analysis: Intergroup comparison was done using Mann–Whitney U-test. Intragroup comparison was done using Wilcoxon signed-rank test. Results: Hydrogen water was nontoxic to the fibroblasts at 24 h and 48 h. The intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (P = 0.00) difference at 24 h and 48 h. Hydrogen water also positively influenced the migratory capacity. Hydrogen water-treated fibroblasts obtained from chronic periodontitis patients showed more migration in comparison to the healthy group (P = 0.00). Hydrogen water showed an antioxidative potential. The maximum potential was seen in relation to the fibroblasts obtained from chronic periodontitis patients at 48 h. Conclusion: Hydrogen water was nontoxic, increased the migratory capacity, and showed an antioxidative potential on human fibroblasts obtained from periodontally healthy individuals and patients with chronic periodontitis.http://www.jisponline.com/article.asp?issn=0972-124X;year=2023;volume=27;issue=3;spage=278;epage=282;aulast=Bhattantioxidantscell migration assayscell survivalchronic periodontitisfibroblasts |
| spellingShingle | Akanksha Bhatt Aarati Nayak Kishore Bhat Chetana Bogar Ranganath Nayak Sachita Naik Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis Journal of Indian Society of Periodontology antioxidants cell migration assays cell survival chronic periodontitis fibroblasts |
| title | Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis |
| title_full | Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis |
| title_fullStr | Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis |
| title_full_unstemmed | Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis |
| title_short | Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis |
| title_sort | assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis |
| topic | antioxidants cell migration assays cell survival chronic periodontitis fibroblasts |
| url | http://www.jisponline.com/article.asp?issn=0972-124X;year=2023;volume=27;issue=3;spage=278;epage=282;aulast=Bhatt |
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