Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800019&lng=en&tlng=en |
| _version_ | 1797712663948558336 |
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| author | Maria Cecília Fernandes Leonardo Rodrigues de Andrade Norma Windsor Andrews Renato Arruda Mortara |
| author_facet | Maria Cecília Fernandes Leonardo Rodrigues de Andrade Norma Windsor Andrews Renato Arruda Mortara |
| author_sort | Maria Cecília Fernandes |
| collection | DOAJ |
| description | It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures. |
| first_indexed | 2024-03-12T07:25:04Z |
| format | Article |
| id | doaj.art-3c3030612dad44b8b739dffabffe5593 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:25:04Z |
| publishDate | 2011-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-3c3030612dad44b8b739dffabffe55932023-09-02T22:11:04ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-12-0110681014101610.1590/S0074-02762011000800019S0074-02762011000800019Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasionMaria Cecília Fernandes0Leonardo Rodrigues de Andrade1Norma Windsor Andrews2Renato Arruda Mortara3University of MarylandUniversity of MarylandUniversity of MarylandUniversidade Federal de São PauloIt has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800019&lng=en&tlng=enTrypanosoma cruzicell invasioncytoskeletonmembrane protrusion |
| spellingShingle | Maria Cecília Fernandes Leonardo Rodrigues de Andrade Norma Windsor Andrews Renato Arruda Mortara Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion Memorias do Instituto Oswaldo Cruz Trypanosoma cruzi cell invasion cytoskeleton membrane protrusion |
| title | Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion |
| title_full | Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion |
| title_fullStr | Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion |
| title_full_unstemmed | Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion |
| title_short | Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion |
| title_sort | trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during hela cell invasion |
| topic | Trypanosoma cruzi cell invasion cytoskeleton membrane protrusion |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000800019&lng=en&tlng=en |
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