Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpa</em> Fruit Extract
An earlier anti-hyperglycemic study with serial crude extracts of <em>Phaleria macrocarpa</em> (PM) fruit indicated methanol extract (ME) as the most effective. In the present investigation, the methanol extract was further fractionated to obtain chloroform (CF), ethyl ac...
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| Format: | Article |
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MDPI AG
2012-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/5/4986 |
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| author | Mohd Z. Asmawi Item J. Atangwho Rabyah B. Ali Roziahanim Mahmud Mariam Ahmad Omar Saad Abraika Navneet Kaur |
| author_facet | Mohd Z. Asmawi Item J. Atangwho Rabyah B. Ali Roziahanim Mahmud Mariam Ahmad Omar Saad Abraika Navneet Kaur |
| author_sort | Mohd Z. Asmawi |
| collection | DOAJ |
| description | An earlier anti-hyperglycemic study with serial crude extracts of <em>Phaleria macrocarpa</em> (PM) fruit indicated methanol extract (ME) as the most effective. In the present investigation, the methanol extract was further fractionated to obtain chloroform (CF), ethyl acetate (EAF), <em>n</em>-butanol (NBF) and aqueous (AF) fractions, which were tested for antidiabetic activity. The NBF reduced blood glucose (<em>p</em> < 0.05) 15 min after administration, in an intraperitoneal glucose tolerance test (IPGTT) similar to metformin. Moreover, it lowered blood glucose in diabetic rats by 66.67% (<em>p</em><em> </em>< 0.05), similar to metformin (51.11%), glibenclamide (66.67%) and insulin (71.43%) after a 12-day treatment, hence considered to be the most active fraction. Further fractionation of NBF yielded sub-fractions I (SFI) and II (SFII), and only SFI lowered blood glucose (<em>p </em>< 0.05), in IPGTT similar to glibenclamide. The ME, NBF, and SFI correspondingly lowered plasma insulin (<em>p </em>< 0.05) and dose-dependently inhibited glucose transport across isolated rat jejunum implying an extra-pancreatic mechanism. Phytochemical screening showed the presence of flavonoids, terpenes and tannins, in ME, NBF and SFI, and LC-MS analyses revealed 9.52%, 33.30% and 22.50% mangiferin respectively. PM fruit possesses anti-hyperglycemic effect, exerted probably through extra-pancreatic action. Magniferin, contained therein may be responsible for this reported activity. |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T01:33:58Z |
| publishDate | 2012-04-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3c36f08eb66744938090702330a9e7392022-12-22T00:42:53ZengMDPI AGMolecules1420-30492012-04-011754986500210.3390/molecules17054986Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpa</em> Fruit ExtractMohd Z. AsmawiItem J. AtangwhoRabyah B. AliRoziahanim MahmudMariam AhmadOmar Saad AbraikaNavneet KaurAn earlier anti-hyperglycemic study with serial crude extracts of <em>Phaleria macrocarpa</em> (PM) fruit indicated methanol extract (ME) as the most effective. In the present investigation, the methanol extract was further fractionated to obtain chloroform (CF), ethyl acetate (EAF), <em>n</em>-butanol (NBF) and aqueous (AF) fractions, which were tested for antidiabetic activity. The NBF reduced blood glucose (<em>p</em> < 0.05) 15 min after administration, in an intraperitoneal glucose tolerance test (IPGTT) similar to metformin. Moreover, it lowered blood glucose in diabetic rats by 66.67% (<em>p</em><em> </em>< 0.05), similar to metformin (51.11%), glibenclamide (66.67%) and insulin (71.43%) after a 12-day treatment, hence considered to be the most active fraction. Further fractionation of NBF yielded sub-fractions I (SFI) and II (SFII), and only SFI lowered blood glucose (<em>p </em>< 0.05), in IPGTT similar to glibenclamide. The ME, NBF, and SFI correspondingly lowered plasma insulin (<em>p </em>< 0.05) and dose-dependently inhibited glucose transport across isolated rat jejunum implying an extra-pancreatic mechanism. Phytochemical screening showed the presence of flavonoids, terpenes and tannins, in ME, NBF and SFI, and LC-MS analyses revealed 9.52%, 33.30% and 22.50% mangiferin respectively. PM fruit possesses anti-hyperglycemic effect, exerted probably through extra-pancreatic action. Magniferin, contained therein may be responsible for this reported activity.http://www.mdpi.com/1420-3049/17/5/4986<em>Phaleria macrocarpa</em>antidiabeticplasma insulinmedicinal plantmangiferin |
| spellingShingle | Mohd Z. Asmawi Item J. Atangwho Rabyah B. Ali Roziahanim Mahmud Mariam Ahmad Omar Saad Abraika Navneet Kaur Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpa</em> Fruit Extract Molecules <em>Phaleria macrocarpa</em> antidiabetic plasma insulin medicinal plant mangiferin |
| title | Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpa</em> Fruit Extract |
| title_full | Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpa</em> Fruit Extract |
| title_fullStr | Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpa</em> Fruit Extract |
| title_full_unstemmed | Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpa</em> Fruit Extract |
| title_short | Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpa</em> Fruit Extract |
| title_sort | bioassay guided antidiabetic study of lt em gt phaleria macrocarpa lt em gt fruit extract |
| topic | <em>Phaleria macrocarpa</em> antidiabetic plasma insulin medicinal plant mangiferin |
| url | http://www.mdpi.com/1420-3049/17/5/4986 |
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