Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a n...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/19/1/52 |
| _version_ | 1818000357690703872 |
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| author | Hee-Jin Jeong Jinhua Dong Hiroshi Ueda |
| author_facet | Hee-Jin Jeong Jinhua Dong Hiroshi Ueda |
| author_sort | Hee-Jin Jeong |
| collection | DOAJ |
| description | We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus. |
| first_indexed | 2024-04-14T03:21:41Z |
| format | Article |
| id | doaj.art-3c37e5f3696946e59e6a7974d10a583f |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T03:21:41Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-3c37e5f3696946e59e6a7974d10a583f2022-12-22T02:15:18ZengMDPI AGSensors1424-82202018-12-011915210.3390/s19010052s19010052Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced QuenchbodiesHee-Jin Jeong0Jinhua Dong1Hiroshi Ueda2Department of Biological and Chemical Engineering, College of Science and Technology, Hongik University, Sejong-si 30016, KoreaKey Laboratory of Biological Medicine in Universities of Shandong Province, School of Bioscience and Technology, Weifang Medical University, Weifang 261042, ChinaLaboratory for Chemistry and Life Science, Institute for Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, JapanWe have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.http://www.mdpi.com/1424-8220/19/1/52influenza AhemagglutininH1N1Quenchbodyimmunosensor |
| spellingShingle | Hee-Jin Jeong Jinhua Dong Hiroshi Ueda Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies Sensors influenza A hemagglutinin H1N1 Quenchbody immunosensor |
| title | Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies |
| title_full | Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies |
| title_fullStr | Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies |
| title_full_unstemmed | Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies |
| title_short | Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies |
| title_sort | single step detection of the influenza virus hemagglutinin using bacterially produced quenchbodies |
| topic | influenza A hemagglutinin H1N1 Quenchbody immunosensor |
| url | http://www.mdpi.com/1424-8220/19/1/52 |
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