Immobilization of laccase on hybrid layered double hydroxide
Crystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2009-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000600025 |
| _version_ | 1811226190909800448 |
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| author | David Isidoro Camacho Córdova Rogelio Morales Borges Gregorio Guadalupe Carbajal Arizaga Fernando Wypych Nádia Krieger |
| author_facet | David Isidoro Camacho Córdova Rogelio Morales Borges Gregorio Guadalupe Carbajal Arizaga Fernando Wypych Nádia Krieger |
| author_sort | David Isidoro Camacho Córdova |
| collection | DOAJ |
| description | Crystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme laccase (Myceliophthora thermophila). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized laccase was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies. |
| first_indexed | 2024-04-12T09:20:12Z |
| format | Article |
| id | doaj.art-3c3b1039aab74abf8f322b9f0ceaf0a7 |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-04-12T09:20:12Z |
| publishDate | 2009-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-3c3b1039aab74abf8f322b9f0ceaf0a72022-12-22T03:38:39ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642009-01-013261495149910.1590/S0100-40422009000600025Immobilization of laccase on hybrid layered double hydroxideDavid Isidoro Camacho CórdovaRogelio Morales BorgesGregorio Guadalupe Carbajal ArizagaFernando WypychNádia KriegerCrystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme laccase (Myceliophthora thermophila). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized laccase was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000600025laccaselayered double hydroxideimmobilization |
| spellingShingle | David Isidoro Camacho Córdova Rogelio Morales Borges Gregorio Guadalupe Carbajal Arizaga Fernando Wypych Nádia Krieger Immobilization of laccase on hybrid layered double hydroxide Química Nova laccase layered double hydroxide immobilization |
| title | Immobilization of laccase on hybrid layered double hydroxide |
| title_full | Immobilization of laccase on hybrid layered double hydroxide |
| title_fullStr | Immobilization of laccase on hybrid layered double hydroxide |
| title_full_unstemmed | Immobilization of laccase on hybrid layered double hydroxide |
| title_short | Immobilization of laccase on hybrid layered double hydroxide |
| title_sort | immobilization of laccase on hybrid layered double hydroxide |
| topic | laccase layered double hydroxide immobilization |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000600025 |
| work_keys_str_mv | AT davidisidorocamachocordova immobilizationoflaccaseonhybridlayereddoublehydroxide AT rogeliomoralesborges immobilizationoflaccaseonhybridlayereddoublehydroxide AT gregorioguadalupecarbajalarizaga immobilizationoflaccaseonhybridlayereddoublehydroxide AT fernandowypych immobilizationoflaccaseonhybridlayereddoublehydroxide AT nadiakrieger immobilizationoflaccaseonhybridlayereddoublehydroxide |