Establishment of an enzymatic hydrolysis evaluation index for dairy cows’ placental hydrolysates
This study aimed to investigate the bioactive polypeptides of dairy cows' placenta and establish an effective enzymatic hydrolysis evaluation index for preparing bioactive hydrolysates. Hydrolysed placental homogenates by trypsin and papain and evaluated hydrolysis time, substrate concentration...
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/09712119.2022.2132950 |
| _version_ | 1828142288421781504 |
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| author | Liu-hong Shen Ying-kun Zhu Liu-chao You Yue Zhang Bo-lin Qian Jin-bang Xiao Li-kou Zou Sui-zhong Cao Guang-neng Peng Shu-min Yu Zhi-cai Zuo Xiao-ping Ma Zhi-jun Zhong Zhi-hua Ren Ya Wang Hai-feng Liu Zi-yao Zhou Dong-jie Cai Xiao-lan Zong Jun-liang Deng |
| author_facet | Liu-hong Shen Ying-kun Zhu Liu-chao You Yue Zhang Bo-lin Qian Jin-bang Xiao Li-kou Zou Sui-zhong Cao Guang-neng Peng Shu-min Yu Zhi-cai Zuo Xiao-ping Ma Zhi-jun Zhong Zhi-hua Ren Ya Wang Hai-feng Liu Zi-yao Zhou Dong-jie Cai Xiao-lan Zong Jun-liang Deng |
| author_sort | Liu-hong Shen |
| collection | DOAJ |
| description | This study aimed to investigate the bioactive polypeptides of dairy cows' placenta and establish an effective enzymatic hydrolysis evaluation index for preparing bioactive hydrolysates. Hydrolysed placental homogenates by trypsin and papain and evaluated hydrolysis time, substrate concentrations and Enzyme/Substrate ratio to hydrolysates extraction rate and also their reducibility by Box-Behnken central response model. Optimal hydrolysis conditions of trypsin and papain for the highest extraction rate and reducibility were as follows: hydrolysis time 5.78, 5.13 h and 6.18, 3.95h; substrate concentrations 30.21, 35.59 %, and 34.78, 22.04 %, E/S 3.24, 4.19% and 5.00, 3.64%, respectively. The corresponding extraction rates and Vit.C equivalent were 5.55% and 39.32 μg/mg for trypsin, 6.97% and 70.54 μg/mg for papain. Correlations between the extraction rate and reducibility were significant in trypsin but not in papain. Optimized hydrolysis conditions with the highest integrated extraction rate (I) of trypsin and papain were as follows; hydrolysis time 5.8 and 4.7h; substrate concentrations 34.98 and 34.03 %; E/S 3.33 and 3.66%, respectively and corresponding integrated extraction rates were 2037.92 and 4414.09 μg·g−1, respectively. These results indicated that papain has higher hydrolysis efficiency than trypsin and I value is applicable for evaluating bioactive enzymatic hydrolysis of the placenta of dairy cows. |
| first_indexed | 2024-04-11T19:38:20Z |
| format | Article |
| id | doaj.art-3c3bad87914343898e94cded919ae303 |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-04-11T19:38:20Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-3c3bad87914343898e94cded919ae3032022-12-22T04:06:46ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442022-12-0150166667710.1080/09712119.2022.2132950Establishment of an enzymatic hydrolysis evaluation index for dairy cows’ placental hydrolysatesLiu-hong Shen0Ying-kun Zhu1Liu-chao You2Yue Zhang3Bo-lin Qian4Jin-bang Xiao5Li-kou Zou6Sui-zhong Cao7Guang-neng Peng8Shu-min Yu9Zhi-cai Zuo10Xiao-ping Ma11Zhi-jun Zhong12Zhi-hua Ren13Ya Wang14Hai-feng Liu15Zi-yao Zhou16Dong-jie Cai17Xiao-lan Zong18Jun-liang Deng19The Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThe Medical Research Center for Cow Disease, The Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaThis study aimed to investigate the bioactive polypeptides of dairy cows' placenta and establish an effective enzymatic hydrolysis evaluation index for preparing bioactive hydrolysates. Hydrolysed placental homogenates by trypsin and papain and evaluated hydrolysis time, substrate concentrations and Enzyme/Substrate ratio to hydrolysates extraction rate and also their reducibility by Box-Behnken central response model. Optimal hydrolysis conditions of trypsin and papain for the highest extraction rate and reducibility were as follows: hydrolysis time 5.78, 5.13 h and 6.18, 3.95h; substrate concentrations 30.21, 35.59 %, and 34.78, 22.04 %, E/S 3.24, 4.19% and 5.00, 3.64%, respectively. The corresponding extraction rates and Vit.C equivalent were 5.55% and 39.32 μg/mg for trypsin, 6.97% and 70.54 μg/mg for papain. Correlations between the extraction rate and reducibility were significant in trypsin but not in papain. Optimized hydrolysis conditions with the highest integrated extraction rate (I) of trypsin and papain were as follows; hydrolysis time 5.8 and 4.7h; substrate concentrations 34.98 and 34.03 %; E/S 3.33 and 3.66%, respectively and corresponding integrated extraction rates were 2037.92 and 4414.09 μg·g−1, respectively. These results indicated that papain has higher hydrolysis efficiency than trypsin and I value is applicable for evaluating bioactive enzymatic hydrolysis of the placenta of dairy cows.https://www.tandfonline.com/doi/10.1080/09712119.2022.2132950Dairy cowenzymatic hydrolysisreducibilityextraction rateplacenta |
| spellingShingle | Liu-hong Shen Ying-kun Zhu Liu-chao You Yue Zhang Bo-lin Qian Jin-bang Xiao Li-kou Zou Sui-zhong Cao Guang-neng Peng Shu-min Yu Zhi-cai Zuo Xiao-ping Ma Zhi-jun Zhong Zhi-hua Ren Ya Wang Hai-feng Liu Zi-yao Zhou Dong-jie Cai Xiao-lan Zong Jun-liang Deng Establishment of an enzymatic hydrolysis evaluation index for dairy cows’ placental hydrolysates Journal of Applied Animal Research Dairy cow enzymatic hydrolysis reducibility extraction rate placenta |
| title | Establishment of an enzymatic hydrolysis evaluation index for dairy cows’ placental hydrolysates |
| title_full | Establishment of an enzymatic hydrolysis evaluation index for dairy cows’ placental hydrolysates |
| title_fullStr | Establishment of an enzymatic hydrolysis evaluation index for dairy cows’ placental hydrolysates |
| title_full_unstemmed | Establishment of an enzymatic hydrolysis evaluation index for dairy cows’ placental hydrolysates |
| title_short | Establishment of an enzymatic hydrolysis evaluation index for dairy cows’ placental hydrolysates |
| title_sort | establishment of an enzymatic hydrolysis evaluation index for dairy cows placental hydrolysates |
| topic | Dairy cow enzymatic hydrolysis reducibility extraction rate placenta |
| url | https://www.tandfonline.com/doi/10.1080/09712119.2022.2132950 |
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